Thr69
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Home > Phosphorylation Site Page: > Thr69  -  Bcl-2 (human)

Site Information
SRDPVARtsPLQtPA   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448398

In vivo Characterization
Methods used to characterize site in vivo:
2D analysis ( 7 ) , [32P] bio-synthetic labeling ( 6 , 7 ) , electrophoretic mobility shift ( 7 ) , mutation of modification site ( 1 , 2 , 3 , 4 , 5 , 6 , 7 ) , peptide sequencing ( 7 ) , phospho-antibody ( 2 ) , phosphoamino acid analysis ( 7 ) , western blotting ( 1 , 2 )
Disease tissue studied:
breast cancer ( 1 , 2 ) , colorectal cancer ( 1 ) , colorectal carcinoma ( 1 ) , leukemia ( 3 , 4 ) , lung cancer ( 4 , 6 ) , non-small cell lung cancer ( 4 ) , small-cell lung cancer ( 6 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Regulatory protein:
JNK1 (human) ( 2 )
Putative in vivo kinases:
JNK1 (human) ( 1 )
Treatments:
amino_acid_starvation ( 2 ) , C2-ceramide ( 1 ) , C2-DHCer ( 1 ) , cisplatin ( 4 ) , nicotine ( 6 ) , taxol ( 7 )

Downstream Regulation
Effects of modification on Bcl-2:
molecular association, regulation ( 1 , 2 , 4 )
Effects of modification on biological processes:
apoptosis, altered ( 5 ) , apoptosis, inhibited ( 3 , 4 )
Induce interaction with:
BAX (mouse) ( 4 )
Inhibit interaction with:
beclin 1 (human) ( 1 , 2 ) , p53 (mouse) ( 4 )

References 

1

Pattingre S, et al. (2009) Role of JNK1-dependent Bcl-2 phosphorylation in ceramide-induced macroautophagy. J Biol Chem 284, 2719-28
19029119   Curated Info

2

Wei Y, et al. (2008) JNK1-mediated phosphorylation of Bcl-2 regulates starvation-induced autophagy. Mol Cell 30, 678-88
18570871   Curated Info

3

Konopleva M, et al. (2006) Mechanisms of apoptosis sensitivity and resistance to the BH3 mimetic ABT-737 in acute myeloid leukemia. Cancer Cell 10, 375-88
17097560   Curated Info

4

Deng X, et al. (2006) Bcl2's flexible loop domain regulates p53 binding and survival. Mol Cell Biol 26, 4421-34
16738310   Curated Info

5

Müller IM, et al. (2005) Cephalostatin 1 inactivates Bcl-2 by hyperphosphorylation independent of M-phase arrest and DNA damage. Mol Pharmacol 67, 1684-9
15703383   Curated Info

6

Mai H, et al. (2003) A functional role for nicotine in Bcl2 phosphorylation and suppression of apoptosis. J Biol Chem 278, 1886-91
12421819   Curated Info

7

Yamamoto K, Ichijo H, Korsmeyer SJ (1999) BCL-2 is phosphorylated and inactivated by an ASK1/Jun N-terminal protein kinase pathway normally activated at G(2)/M. Mol Cell Biol 19, 8469-78
10567572   Curated Info