Thr845
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Home > Phosphorylation Site Page: > Thr845  -  ASK1 (mouse)

Site Information
GINPCtEtFTGtLQY   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448394

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 4 , 5 ) , mutation of modification site ( 4 , 6 ) , phospho-antibody ( 1 , 2 , 3 , 4 , 5 , 6 , 7 ) , western blotting ( 1 , 2 , 3 , 4 , 5 , 6 )
Relevant cell line - cell type - tissue:
293 (epithelial) ( 1 , 2 , 3 , 4 , 5 , 7 ) , 3T3 (fibroblast) ( 2 ) , BAEC (endothelial) ( 6 ) , E.coli (bacterial) ( 5 ) , HEK293T (epithelial) ( 4 ) , MEF (fibroblast) ( 1 , 6 ) , MEF (fibroblast) [IGF1R (mouse)] ( 7 )

Upstream Regulation
Regulatory protein:
Akt1 (mouse) ( 6 ) , HSP90A (human) ( 6 ) , PGAM5 (mouse) ( 3 ) , STRAP (human) ( 2 )
Putative in vivo kinases:
ASK1 (mouse) ( 7 ) , MEKK6 (human) ( 5 ) , MELK (mouse) ( 4 )
Kinases, in vitro:
ASK1 (mouse) ( 7 ) , MELK (mouse) ( 4 ) , PDK1 (human) ( 1 )
Treatments:
H2O2 ( 2 , 6 , 7 )

Downstream Regulation
Effects of modification on ASK1:
enzymatic activity, induced ( 4 , 5 ) , molecular association, regulation ( 4 )
Effects of modification on biological processes:
apoptosis, induced ( 4 ) , transcription, induced ( 4 )
Induce interaction with:
MKK3 (mouse) ( 4 )

References 

1

Seong HA, Jung H, Ichijo H, Ha H (2010) Reciprocal negative regulation of PDK1 and ASK1 signaling by direct interaction and phosphorylation. J Biol Chem 285, 2397-414
19920149   Curated Info

2

Jung H, Seong HA, Manoharan R, Ha H (2010) Serine-threonine kinase receptor-associated protein inhibits apoptosis signal-regulating kinase 1 function through direct interaction. J Biol Chem 285, 54-70
19880523   Curated Info

3

Takeda K, et al. (2009) Mitochondrial phosphoglycerate mutase 5 uses alternate catalytic activity as a protein serine/threonine phosphatase to activate ASK1. Proc Natl Acad Sci U S A 106, 12301-5
19590015   Curated Info

4

Jung H, Seong HA, Ha H (2008) Murine protein serine/threonine kinase 38 activates apoptosis signal-regulating kinase 1 via Thr 838 phosphorylation. J Biol Chem 283, 34541-53
18948261   Curated Info

5

Takeda K, et al. (2007) Apoptosis signal-regulating kinase (ASK) 2 functions as a mitogen-activated protein kinase kinase kinase in a heteromeric complex with ASK1. J Biol Chem 282, 7522-31
17210579   Curated Info

6

Zhang R, et al. (2005) Hsp90-Akt phosphorylates ASK1 and inhibits ASK1-mediated apoptosis. Oncogene 24, 3954-63
15782121   Curated Info

7

Tobiume K, Saitoh M, Ichijo H (2002) Activation of apoptosis signal-regulating kinase 1 by the stress-induced activating phosphorylation of pre-formed oligomer. J Cell Physiol 191, 95-104
11920685   Curated Info