Tyr504
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Home > Phosphorylation Site Page: > Tyr504  -  RAPGEF1 (human)

Site Information
APIPSVPyAPFAAIL   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448393

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 10 , 12 ) , flow cytometry ( 3 ) , immunoassay ( 2 , 6 , 8 ) , immunoprecipitation ( 3 , 5 , 6 , 7 ) , mutation of modification site ( 3 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 ) , phospho-antibody ( 2 , 3 , 4 , 6 , 7 , 8 , 9 , 10 , 11 ) , western blotting ( 2 , 3 , 4 , 5 , 6 , 7 , 8 , 11 )
Disease tissue studied:
leukemia ( 2 , 3 , 5 ) , chronic myelogenous leukemia ( 2 ) , T cell leukemia ( 3 , 5 ) , lymphoma ( 3 ) , Burkitt's lymphoma ( 3 ) , neuroblastoma ( 6 , 8 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Regulatory protein:
CRK (human) ( 7 )
Putative in vivo kinases:
Abl (human) ( 7 ) , Hck (human) ( 5 , 9 ) , Src (human) ( 6 , 9 )
Kinases, in vitro:
Abl (human) ( 7 ) , Hck (human) ( 10 )
Putative upstream phosphatases:
PTPN2 (human) ( 6 )
Treatments:
anti-CD3/CD28 ( 4 ) , colforsin ( 8 ) , H2O2 ( 7 ) , ICAM-1 ( 4 ) , IGF-1 ( 6 ) , imatinib ( 7 ) , mercuric_chloride ( 10 ) , NGF ( 8 ) , phorbol_ester ( 2 ) , PP2 ( 8 ) , staurosporine ( 7 ) , vanadate ( 6 , 7 , 8 , 9 ) , Z-VAD-FMK ( 7 )

Downstream Regulation
Effects of modification on RAPGEF1:
activity, induced ( 2 , 7 , 11 , 12 ) , enzymatic activity, induced ( 8 ) , intracellular localization ( 3 , 5 , 6 , 7 , 9 ) , phosphorylation ( 11 )
Effects of modification on biological processes:
apoptosis, altered ( 7 ) , cell cycle regulation ( 11 ) , cell differentiation, induced ( 8 ) , cell growth, induced ( 6 , 8 ) , transcription, altered ( 11 )

References 

1

GutiƩrrez-Herrero S, et al. (2020) C3G contributes to platelet activation and aggregation by regulating major signaling pathways. Signal Transduct Target Ther 5, 29
32296045   Curated Info

2

Ortiz-Rivero S, et al. (2018) C3G, through its GEF activity, induces megakaryocytic differentiation and proplatelet formation. Cell Commun Signal 16, 101
30567575   Curated Info

3

Azoulay-Alfaguter I, et al. (2017) The tyrosine phosphatase SHP-1 promotes T cell adhesion by activating the adaptor protein CrkII in the immunological synapse. Sci Signal 10
28790195   Curated Info

4

Eppler FJ, Quast T, Kolanus W (2017) Dynamin2 controls Rap1 activation and integrin clustering in human T lymphocyte adhesion. PLoS One 12, e0172443
28273099   Curated Info

5

Kloog Y, Mor A (2014) Cytotoxic-T-Lymphocyte Antigen 4 Receptor Signaling for Lymphocyte Adhesion Is Mediated by C3G and Rap1. Mol Cell Biol 34, 978-88
24396067   Curated Info

6

Mitra A, Kalayarasan S, Gupta V, Radha V (2011) TC-PTP Dephosphorylates the Guanine Nucleotide Exchange Factor C3G (RapGEF1) and Negatively Regulates Differentiation of Human Neuroblastoma Cells. PLoS One 6, e23681
21876762   Curated Info

7

Mitra A, Radha V (2010) F-actin-binding domain of c-Abl regulates localized phosphorylation of C3G: role of C3G in c-Abl-mediated cell death. Oncogene 29, 4528-42
20581864   Curated Info

8

Radha V, et al. (2008) The guanine nucleotide exchange factor, C3G regulates differentiation and survival of human neuroblastoma cells. J Neurochem 107, 1424-35
18957052   Curated Info

9

Radha V, Rajanna A, Swarup G (2004) Phosphorylated guanine nucleotide exchange factor C3G, induced by pervanadate and Src family kinases localizes to the Golgi and subcortical actin cytoskeleton. BMC Cell Biol 5, 31
15320955   Curated Info

10

Shivakrupa R, Radha V, Sudhakar Ch, Swarup G (2003) Physical and functional interaction between Hck tyrosine kinase and guanine nucleotide exchange factor C3G results in apoptosis, which is independent of C3G catalytic domain. J Biol Chem 278, 52188-94
14551197   Curated Info

11

Ling L, Zhu T, Lobie PE (2003) Src-CrkII-C3G-dependent activation of Rap1 switches growth hormone-stimulated p44/42 MAP kinase and JNK/SAPK activities. J Biol Chem 278, 27301-11
12734187   Curated Info

12

Ichiba T, et al. (1999) Activation of C3G guanine nucleotide exchange factor for Rap1 by phosphorylation of tyrosine 504. J Biol Chem 274, 14376-81
10318861   Curated Info