Tyr452

Site Information
STNsEDNyVPMNPGs SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 448384
Available spectra: 1 CST
Associated spectra: 1 CST

In vivo Characterization
Methods used to characterize site in vivo:	mass spectrometry ( 2 , 3 , 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 ) , mutation of modification site ( 34 ) , phospho-antibody ( 1 ) , western blotting ( 1 )
Disease tissue studied:	breast cancer ( 11 , 12 ) , HER2 positive breast cancer ( 2 ) , luminal B breast cancer ( 2 ) , colorectal cancer ( 14 , 15 ) , colorectal carcinoma ( 14 , 15 ) , gallbladder cancer ( 9 ) , gastric cancer ( 6 , 7 ) , gastric carcinoma ( 6 , 7 ) , leukemia ( 5 , 10 , 17 , 18 , 19 , 20 , 21 , 22 , 23 , 27 , 28 , 29 , 31 ) , acute lymphocytic leukemia ( 8 , 20 , 21 , 22 , 23 , 28 , 31 ) , acute myelogenous leukemia ( 17 , 18 , 19 , 27 , 29 ) , chronic myelogenous leukemia ( 5 , 10 ) , liver cancer ( 1 ) , hepatocellular carcinoma ( 1 ) , lung cancer ( 16 , 24 , 25 , 32 , 33 ) , non-small cell lung cancer ( 4 , 16 , 25 , 33 ) , non-small cell lung adenocarcinoma ( 4 ) , non-small cell squamous cell lung carcinoma ( 33 ) , small-cell lung cancer ( 24 , 32 ) , lymphoma ( 26 , 30 ) , anaplastic large cell lymphoma ( 26 , 30 ) , neuroblastoma ( 3 , 13 )
Relevant cell line - cell type - tissue:	breast ( 2 ) , CAL-51 (breast cell) ( 11 , 12 ) , CMK (megakaryoblast) ( 17 ) , DND-41 (T lymphocyte) ( 21 , 22 ) , GI-LI-N (neuron) ( 3 , 13 ) , Huh7 (hepatic) ( 1 ) , K562 (erythroid) ( 5 , 10 ) , KOPT-K1 (T lymphocyte) ( 23 ) , MKN-45 (gastric) ( 6 , 7 ) , MV4-11 (macrophage) ( 18 ) , NCI-H1435 (pulmonary) ( 25 ) , NCI-H2228 (pulmonary) ( 4 ) , NCI-H226 (pulmonary) ( 33 ) , NCI-H2286 (pulmonary) ( 24 ) , NCI-H596 (pulmonary) ( 16 ) , NCI-H69 (pulmonary) ( 32 ) , NKM-1 (myeloid) ( 19 , 27 ) , NOZ ('epithelial, gallbladder') ( 9 ) , OCI-M1 (myeloid) ( 29 ) , SEM (B lymphocyte) ( 8 , 20 , 28 , 31 ) , SK-N-BE(2) (neural crest) ( 3 ) , SNU-C2B (intestinal) ( 14 , 15 ) , TS (lymphocyte) ( 26 , 30 )

Upstream Regulation
Regulatory protein:	SPHK2 (human) ( 1 )

Downstream Regulation
Effects of modification on GAB2:	molecular association, regulation ( 34 )
Induce interaction with:	PIK3R1 (human) ( 34 )

References
1	Aji G, et al. (2020) Regulation of hepatic insulin signaling and glucose homeostasis by sphingosine kinase 2. Proc Natl Acad Sci U S A 32917816 Curated Info
2	Mertins P, et al. (2016) Proteogenomics connects somatic mutations to signalling in breast cancer. Nature 534, 55-62 27251275 Curated Info
3	Palacios-Moreno J, et al. (2015) Neuroblastoma Tyrosine Kinase Signaling Networks Involve FYN and LYN in Endosomes and Lipid Rafts. PLoS Comput Biol 11, e1004130 25884760 Curated Info
4	Rikova K (2013) CST Curation Set: 18449; Year: 2012; Biosample/Treatment: cell line, H2228/crizotinib, geldanamycin; Disease: -; TMT: Y; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine (P-Tyr-1000) MultiMab(TM) Rabbit mAb mix Cat#: 8954 Curated Info
5	Mulhern D (2011) CST Curation Set: 13266; Year: 2011; Biosample/Treatment: cell line, K-562/untreated &'\|\|' untreated; Disease: chronic myelogenous leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[STY]) Curated Info
6	Moritz A (2011) CST Curation Set: 12518; Year: 2011; Biosample/Treatment: cell line, MKN-45/untreated; Disease: gastric carcinoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info
7	Moritz A (2011) CST Curation Set: 12519; Year: 2011; Biosample/Treatment: cell line, MKN-45/untreated; Disease: gastric carcinoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info
8	Gu TL, et al. (2011) Survey of activated FLT3 signaling in leukemia. PLoS One 6, e19169 21552520 Curated Info
9	Tucker M (2009) CST Curation Set: 5949; Year: 2009; Biosample/Treatment: cell line, NOZ/untreated; Disease: gallbladder cancer; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info
10	Moritz A (2006) CST Curation Set: 2020; Year: 2006; Biosample/Treatment: cell line, K562/-; Disease: chronic myelogenous leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info
11	Michaud C (2006) CST Curation Set: 1988; Year: 2006; Biosample/Treatment: cell line, CAL-51/serum starved; Disease: breast cancer; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info
12	Michaud C (2006) CST Curation Set: 1989; Year: 2006; Biosample/Treatment: cell line, CAL-51/serum starved; Disease: breast cancer; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info
13	Stokes M (2006) CST Curation Set: 1945; Year: 2006; Biosample/Treatment: cell line, GI-LI-N/serum starved; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info
14	Li Y (2006) CST Curation Set: 1656; Year: 2006; Biosample/Treatment: cell line, SNU-C2B/untreated; Disease: colorectal carcinoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info
15	Li Y (2006) CST Curation Set: 1657; Year: 2006; Biosample/Treatment: cell line, SNU-C2B/untreated &'\|\|' TSA; Disease: colorectal carcinoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info
16	Moritz A (2006) CST Curation Set: 1523; Year: 2006; Biosample/Treatment: cell line, NCI-H596/serum starved; Disease: chronic myelogenous leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info
17	Gu T (2006) CST Curation Set: 1460; Year: 2006; Biosample/Treatment: cell line, CMK/untreated; Disease: acute myelogenous leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info
18	Gu T (2006) CST Curation Set: 1413; Year: 2006; Biosample/Treatment: cell line, MV4-11/untreated; Disease: acute myelogenous leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info
19	Gu T (2006) CST Curation Set: 1414; Year: 2006; Biosample/Treatment: cell line, NKM-1/untreated; Disease: leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info
20	Gu T (2006) CST Curation Set: 1415; Year: 2006; Biosample/Treatment: cell line, SEM/untreated; Disease: acute lymphocytic leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info
21	Gu T (2006) CST Curation Set: 1129; Year: 2006; Biosample/Treatment: cell line, DND-41/-; Disease: acute lymphocytic leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info
22	Gu T (2006) CST Curation Set: 1130; Year: 2006; Biosample/Treatment: cell line, DND-41/-; Disease: acute lymphocytic leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info
23	Gu T (2006) CST Curation Set: 1132; Year: 2006; Biosample/Treatment: cell line, KOPT-K1/-; Disease: acute lymphocytic leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info
24	Rikova K (2006) CST Curation Set: 1074; Year: 2006; Biosample/Treatment: cell line, NCI-H2286/serum starved; Disease: small-cell lung cancer; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info
25	Rikova K (2005) CST Curation Set: 985; Year: 2005; Biosample/Treatment: cell line, NCI-H1435/serum starved; Disease: non-small cell lung cancer; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info
26	Goss V (2005) CST Curation Set: 976; Year: 2005; Biosample/Treatment: cell line, TS/B2; Disease: anaplastic large cell lymphoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info
27	Gu T (2005) CST Curation Set: 818; Year: 2005; Biosample/Treatment: cell line, NKM-1/-; Disease: leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info
28	Gu T (2005) CST Curation Set: 628; Year: 2005; Biosample/Treatment: cell line, SEM/-; Disease: acute lymphocytic leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info
29	Goss V (2005) CST Curation Set: 576; Year: 2005; Biosample/Treatment: cell line, OCI-M1/-; Disease: acute myelogenous leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info
30	Boccalatte F (2005) CST Curation Set: 530; Year: 2005; Biosample/Treatment: cell line, TS/-; Disease: anaplastic large cell lymphoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info
31	Gu T (2005) CST Curation Set: 515; Year: 2005; Biosample/Treatment: cell line, SEM/-; Disease: acute lymphocytic leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info
32	Farnsworth C (2004) CST Curation Set: 609; Year: 2004; Biosample/Treatment: cell line, NCI-H69/SCF (stem cell factor); Disease: small-cell lung cancer; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info
33	Farnsworth C (2004) CST Curation Set: 325; Year: 2004; Biosample/Treatment: cell line, NCI-H226/EGF; Disease: non-small cell squamous cell lung carcinoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info
34	Crouin C, et al. (2001) A yeast two-hybrid study of human p97/Gab2 interactions with its SH2 domain-containing binding partners. FEBS Lett 495, 148-53 11334882 Curated Info