Ser29
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage PhosphoSitePlus® v6.6.0.4
Powered by Cell Signaling Technology
Home > Phosphorylation Site Page: > Ser29  -  RFA2 (human)

Site Information
QsPGGFGsPAPsQAE   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448338

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 13 ) , electrophoretic mobility shift ( 13 ) , flow cytometry ( 4 , 12 ) , immunoassay ( 2 ) , immunoprecipitation ( 2 , 7 , 8 ) , mass spectrometry ( 3 , 4 , 5 , 9 ) , mutation of modification site ( 2 , 7 , 8 , 12 ) , peptide sequencing ( 13 ) , phospho-antibody ( 2 , 4 , 6 , 8 , 10 , 11 , 12 ) , phosphopeptide mapping ( 13 ) , western blotting ( 2 , 4 , 6 , 7 , 8 , 10 , 11 , 12 )
Disease tissue studied:
ataxia-telangiectasia ( 11 ) , bone cancer ( 2 , 4 , 6 , 8 , 10 , 12 ) , HER2 positive breast cancer ( 3 ) , luminal A breast cancer ( 3 ) , luminal B breast cancer ( 3 ) , breast cancer, surrounding tissue ( 3 ) , breast cancer, triple negative ( 3 ) , cervical cancer ( 9 , 11 ) , cervical adenocarcinoma ( 9 , 11 ) , colorectal cancer ( 6 ) , colorectal carcinoma ( 6 ) , lung cancer ( 2 ) , non-small cell lung cancer ( 2 ) , non-small cell lung adenocarcinoma ( 2 ) , Seckel syndrome ( 11 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Regulatory protein:
APC (human) ( 6 ) , CDK2 (human) ( 2 ) , CDK5 (human) ( 4 ) , p21Cip1 (human) ( 2 ) , p53 (human) ( 2 ) , PPP4R2 (human) ( 8 )
Putative in vivo kinases:
ATM (human) ( 11 ) , DNAPK (human) ( 11 )
Kinases, in vitro:
CDK1 (human) ( 4 , 11 , 14 ) , CDK2 (human) ( 4 ) , CDK5 (human) ( 4 )
Treatments:
camptothecin ( 2 ) , CPT ( 12 ) , hydroxyurea ( 4 , 6 , 8 , 10 ) , ionizing_radiation ( 11 ) , KU-55933 ( 11 ) , nocodazole ( 9 , 11 , 12 ) , NU7441 ( 11 ) , seliciclib ( 12 ) , U0126 ( 12 ) , UV ( 13 ) , wortmannin ( 11 )

Downstream Regulation
Effects of modification on RFA2:
intracellular localization ( 11 ) , molecular association, regulation ( 2 , 7 )
Effects of modification on biological processes:
cell cycle regulation ( 2 , 4 , 8 , 12 ) , DNA repair, induced ( 2 , 6 , 7 , 12 ) , transcription, altered ( 2 )
Induce interaction with:
WRN (human) ( 7 ) , p53 (human) ( 2 )

References 

1

Liu L, et al. (2019) O-GlcNAcylation of Thr12 / Ser56 in short form O-GlcNAc transferase (sOGT) regulates its substrate selectivity. J Biol Chem
31527085   Curated Info

2

Romanova LY, Mushinski F, Kovalchuk AL (2018) Transcriptional activation of p21 contributes to suppression of HR by p53 in response to replication arrest induced by camptothecin. Oncotarget 9, 25427-25440
29875999   Curated Info

3

Mertins P, et al. (2016) Proteogenomics connects somatic mutations to signalling in breast cancer. Nature 534, 55-62
27251275   Curated Info

4

Chiker S, et al. (2015) Cdk5 promotes DNA replication stress checkpoint activation through RPA-32 phosphorylation, and impacts on metastasis free survival in breast cancer patients. Cell Cycle 14, 3066-78
26237679   Curated Info

5

Mertins P, et al. (2013) Integrated proteomic analysis of post-translational modifications by serial enrichment. Nat Methods 10, 634-7
23749302   Curated Info

6

Brocardo MG, Borowiec JA, Henderson BR (2011) Adenomatous polyposis coli protein regulates the cellular response to DNA replication stress. Int J Biochem Cell Biol 43, 1354-64
21664290   Curated Info

7

Machwe A, et al. (2011) Molecular cooperation between the Werner syndrome protein and replication protein A in relation to replication fork blockage. J Biol Chem 286, 3497-508
21107010   Curated Info

8

Lee DH, et al. (2010) A PP4 phosphatase complex dephosphorylates RPA2 to facilitate DNA repair via homologous recombination. Nat Struct Mol Biol 17, 365-72
20154705   Curated Info

9

Olsen JV, et al. (2010) Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis. Sci Signal 3, ra3
20068231   Curated Info

10

Vassin VM, et al. (2009) Human RPA phosphorylation by ATR stimulates DNA synthesis and prevents ssDNA accumulation during DNA-replication stress. J Cell Sci 122, 4070-80
19843584   Curated Info

11

Stephan H, et al. (2009) Ionizing radiation-dependent and independent phosphorylation of the 32-kDa subunit of replication protein A during mitosis. Nucleic Acids Res 37, 6028-41
19671522   Curated Info

12

Anantha RW, Vassin VM, Borowiec JA (2007) Sequential and Synergistic Modification of Human RPA Stimulates Chromosomal DNA Repair. J Biol Chem 282, 35910-23
17928296   Curated Info

13

Zernik-Kobak M, et al. (1997) Sites of UV-induced phosphorylation of the p34 subunit of replication protein A from HeLa cells. J Biol Chem 272, 23896-904
9295339   Curated Info

14

Niu H, et al. (1997) Mapping of amino acid residues in the p34 subunit of human single-stranded DNA-binding protein phosphorylated by DNA-dependent protein kinase and Cdc2 kinase in vitro. J Biol Chem 272, 12634-41
9139719   Curated Info