Ser23
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Home > Phosphorylation Site Page: > Ser23  -  RFA2 (human)

Site Information
GAGGYtQsPGGFGsP   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448337

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 9 ) , electrophoretic mobility shift ( 9 ) , flow cytometry ( 1 , 8 ) , immunoprecipitation ( 3 , 4 ) , mass spectrometry ( 1 , 2 , 5 ) , mutation of modification site ( 3 , 4 , 8 ) , peptide sequencing ( 9 ) , phospho-antibody ( 1 , 4 , 6 , 7 , 8 ) , phosphopeptide mapping ( 9 ) , western blotting ( 1 , 3 , 4 , 6 , 7 , 8 )
Disease tissue studied:
ataxia-telangiectasia ( 7 ) , bone cancer ( 1 , 4 , 6 , 8 ) , cervical cancer ( 5 , 7 ) , cervical adenocarcinoma ( 5 , 7 ) , Seckel syndrome ( 7 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Regulatory protein:
PPP4R2 (human) ( 4 )
Putative in vivo kinases:
ATM (human) ( 7 ) , DNAPK (human) ( 7 )
Kinases, in vitro:
CDK1 (human) ( 1 , 7 ) , CDK2 (human) ( 1 ) , CDK5 (human) ( 1 )
Treatments:
CPT ( 8 ) , hydroxyurea ( 4 , 6 ) , ionizing_radiation ( 7 ) , KU-55933 ( 7 ) , nocodazole ( 5 , 7 ) , NU7441 ( 7 ) , seliciclib ( 8 ) , UV ( 9 ) , wortmannin ( 7 )

Downstream Regulation
Effects of modification on RFA2:
intracellular localization ( 7 ) , molecular association, regulation ( 3 )
Effects of modification on biological processes:
cell cycle regulation ( 1 , 4 , 8 ) , DNA repair, induced ( 3 , 8 )
Induce interaction with:
WRN (human) ( 3 )

References 

1

Chiker S, et al. (2015) Cdk5 promotes DNA replication stress checkpoint activation through RPA-32 phosphorylation, and impacts on metastasis free survival in breast cancer patients. Cell Cycle 14, 3066-78
26237679   Curated Info

2

Mertins P, et al. (2013) Integrated proteomic analysis of post-translational modifications by serial enrichment. Nat Methods 10, 634-7
23749302   Curated Info

3

Machwe A, et al. (2011) Molecular cooperation between the Werner syndrome protein and replication protein A in relation to replication fork blockage. J Biol Chem 286, 3497-508
21107010   Curated Info

4

Lee DH, et al. (2010) A PP4 phosphatase complex dephosphorylates RPA2 to facilitate DNA repair via homologous recombination. Nat Struct Mol Biol 17, 365-72
20154705   Curated Info

5

Olsen JV, et al. (2010) Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis. Sci Signal 3, ra3
20068231   Curated Info

6

Vassin VM, et al. (2009) Human RPA phosphorylation by ATR stimulates DNA synthesis and prevents ssDNA accumulation during DNA-replication stress. J Cell Sci 122, 4070-80
19843584   Curated Info

7

Stephan H, et al. (2009) Ionizing radiation-dependent and independent phosphorylation of the 32-kDa subunit of replication protein A during mitosis. Nucleic Acids Res 37, 6028-41
19671522   Curated Info

8

Anantha RW, Vassin VM, Borowiec JA (2007) Sequential and Synergistic Modification of Human RPA Stimulates Chromosomal DNA Repair. J Biol Chem 282, 35910-23
17928296   Curated Info

9

Zernik-Kobak M, et al. (1997) Sites of UV-induced phosphorylation of the p34 subunit of replication protein A from HeLa cells. J Biol Chem 272, 23896-904
9295339   Curated Info