Ser380
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Home > Phosphorylation Site Page: > Ser380  -  PTEN (human)

Site Information
EPDHyRYsDttDsDP   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448313

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 24 , 25 ) , flow cytometry ( 5 ) , immunoprecipitation ( 1 , 4 , 5 , 7 , 8 , 14 , 23 ) , mutation of modification site ( 1 , 5 , 6 , 8 , 15 , 17 , 18 , 19 , 22 , 23 , 24 , 25 ) , peptide sequencing ( 2 ) , phospho-antibody ( 3 , 4 , 5 , 6 , 7 , 8 , 9 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 19 , 20 , 21 , 22 , 23 ) , phosphoamino acid analysis ( 23 , 25 ) , phosphopeptide mapping ( 17 ) , western blotting ( 1 , 2 , 3 , 4 , 5 , 6 , 7 , 8 , 9 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 19 , 21 , 22 , 23 )
Disease tissue studied:
breast cancer ( 12 , 13 , 15 , 21 , 22 ) , cervical cancer ( 15 ) , cervical adenocarcinoma ( 15 ) , colorectal cancer ( 2 ) , colorectal carcinoma ( 2 ) , gastric cancer ( 4 ) , gastric carcinoma ( 4 ) , leukemia ( 3 ) , acute myelogenous leukemia ( 3 ) , lymphoma ( 9 ) , PEL ( 9 ) , neuroblastoma ( 11 , 14 ) , ovarian cancer ( 16 ) , ovarian epithelial carcinoma ( 16 ) , pancreatic cancer ( 12 ) , pancreatic carcinoma ( 12 ) , prostate cancer ( 6 ) , Kaposi's sarcoma ( 9 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Regulatory protein:
Notch 1 (human) ( 4 ) , PINK1 (human) ( 11 ) , PKCZ (human) ( 15 ) , PTEN (human) ( 22 , 23 ) , Src (human) ( 22 )
Putative in vivo kinases:
LKB1 (human) ( 15 ) , PLK1 (human) ( 5 )
Kinases, in vitro:
CK2A1 (human) ( 24 ) , LKB1 (human) ( 15 ) , PKCZ (human) ( 15 ) , PLK1 (human) ( 5 )
Putative upstream phosphatases:
INPP4B (human) ( 2 ) , PTEN (human) ( 8 )
Phosphatases, in vitro:
PTEN (human) ( 8 )
Treatments:
4-HT ( 13 ) , 9cRA ( 13 ) , cathepsin_G ( 19 ) , dexamethasone ( 13 ) , EF24 ( 16 ) , EGF ( 11 ) , estrogen_deprivation ( 21 ) , gamma-secretase_inhibitor ( 4 ) , H2O2 ( 7 ) , miRNA ( 3 ) , ONOO(-) ( 15 ) , PKC-zeta_inhibitor ( 15 ) , retinoic_acid ( 13 ) , sardomozide ( 14 ) , silmitasertib ( 12 ) , Sin-1 ( 15 ) , siRNA ( 15 ) , tamoxifen ( 13 ) , taxol ( 4 ) , wortmannin ( 23 )

Downstream Regulation
Effects of modification on PTEN:
enzymatic activity, inhibited ( 17 , 25 ) , intracellular localization ( 4 ) , molecular association, regulation ( 5 ) , protein conformation ( 6 ) , protein stabilization ( 23 , 24 , 25 )
Effects of modification on biological processes:
cell cycle regulation ( 4 , 5 ) , cell differentiation, altered ( 18 ) , cell motility, altered ( 18 ) , neural plasticity ( 8 ) , transcription, altered ( 25 )
Induce interaction with:
DNA ( 5 ) , PTEN (human) ( 17 )
Inhibit interaction with:
PTEN (human) ( 17 )

Disease / Diagnostics Relevance
Relevant diseases:
PEL ( 9 ) , Kaposi's sarcoma ( 9 )

References 

1

Yang JM, et al. (2017) Characterization of PTEN mutations in brain cancer reveals that pten mono-ubiquitination promotes protein stability and nuclear localization. Oncogene 36, 3673-3685
28263967   Curated Info

2

Guo ST, et al. (2016) INPP4B is an oncogenic regulator in human colon cancer. Oncogene 35, 3049-61
26411369   Curated Info

3

Lechman ER, et al. (2016) miR-126 Regulates Distinct Self-Renewal Outcomes in Normal and Malignant Hematopoietic Stem Cells. Cancer Cell 29, 214-28
26832662   Curated Info

4

Kim SJ, et al. (2016) Activation of nuclear PTEN by inhibition of Notch signaling induces G2/M cell cycle arrest in gastric cancer. Oncogene 35, 251-60
25823029   Curated Info

5

Choi BH, Pagano M, Dai W (2014) Plk1 Protein Phosphorylates Phosphatase and Tensin Homolog (PTEN) and Regulates Its Mitotic Activity during the Cell Cycle. J Biol Chem 289, 14066-74
24706748   Curated Info

6

Papa A, et al. (2014) Cancer-Associated PTEN Mutants Act in a Dominant-Negative Manner to Suppress PTEN Protein Function. Cell 157, 595-610
24766807   Curated Info

7

Choi BH, Chen Y, Dai W (2013) Chromatin PTEN is involved in DNA damage response partly through regulating Rad52 sumoylation. Cell Cycle 12, 3442-7
24047694   Curated Info

8

Zhang XC, et al. (2012) Functional analysis of the protein phosphatase activity of PTEN. Biochem J 444, 457-64
22413754   Curated Info

9

Roy D, Dittmer DP (2011) Phosphatase and tensin homolog on chromosome 10 is phosphorylated in primary effusion lymphoma and Kaposi's sarcoma. Am J Pathol 179, 2108-19
21819957   Curated Info

10

Zhang P, et al. (2011) Cross-talk between phospho-STAT3 and PLCĪ³1 plays a critical role in colorectal tumorigenesis. Mol Cancer Res 9, 1418-28
21840932   Curated Info

11

Murata H, et al. (2011) A new cytosolic pathway from a Parkinson disease-associated kinase, BRPK/PINK1: activation of AKT via mTORC2. J Biol Chem 286, 7182-9
21177249   Curated Info

12

Siddiqui-Jain A, et al. (2010) CX-4945, an orally bioavailable selective inhibitor of protein kinase CK2, inhibits prosurvival and angiogenic signaling and exhibits antitumor efficacy. Cancer Res 70, 10288-98
21159648   Curated Info

13

Eto I (2010) Upstream molecular signaling pathways of p27(Kip1) expression: Effects of 4-hydroxytamoxifen, dexamethasone, and retinoic acids. Cancer Cell Int 10, 3
20170512   Curated Info

14

Koomoa DL, et al. (2008) Ornithine decarboxylase inhibition by alpha-difluoromethylornithine activates opposing signaling pathways via phosphorylation of both Akt/protein kinase B and p27Kip1 in neuroblastoma. Cancer Res 68, 9825-31
19047162   Curated Info

15

Song P, et al. (2008) Protein kinase Czeta-dependent LKB1 serine 428 phosphorylation increases LKB1 nucleus export and apoptosis in endothelial cells. J Biol Chem 283, 12446-55
18321849   Curated Info

16

Selvendiran K, et al. (2007) EF24 induces G2/M arrest and apoptosis in cisplatin-resistant human ovarian cancer cells by increasing PTEN expression. J Biol Chem 282, 28609-18
17684018   Curated Info

17

Odriozola L, Singh G, Hoang T, Chan AM (2007) Regulation of PTEN activity by its carboxyl-terminal autoinhibitory domain. J Biol Chem 282, 23306-15
17565999   Curated Info

18

Leslie NR, Yang X, Downes CP, Weijer CJ (2007) PtdIns(3,4,5)P(3)-dependent and -independent roles for PTEN in the control of cell migration. Curr Biol 17, 115-25
17240336   Curated Info

19

Rafiq K, et al. (2006) Role of protein-tyrosine phosphatase SHP2 in focal adhesion kinase down-regulation during neutrophil cathepsin G-induced cardiomyocytes anoikis. J Biol Chem 281, 19781-92
16690621   Curated Info

20

Al-Khouri AM, et al. (2005) Cooperative phosphorylation of the tumor suppressor phosphatase and tensin homologue (PTEN) by casein kinases and glycogen synthase kinase 3beta. J Biol Chem 280, 35195-202
16107342   Curated Info

21

Sabnis GJ, Jelovac D, Long B, Brodie A (2005) The role of growth factor receptor pathways in human breast cancer cells adapted to long-term estrogen deprivation. Cancer Res 65, 3903-10
15867390   Curated Info

22

Lu Y, et al. (2003) Src family protein-tyrosine kinases alter the function of PTEN to regulate phosphatidylinositol 3-kinase/AKT cascades. J Biol Chem 278, 40057-66
12869565   Curated Info

23

Birle D, et al. (2002) Negative feedback regulation of the tumor suppressor PTEN by phosphoinositide-induced serine phosphorylation. J Immunol 169, 286-91
12077256   Curated Info

24

Torres J, Pulido R (2001) The tumor suppressor PTEN is phosphorylated by the protein kinase CK2 at its C terminus. Implications for PTEN stability to proteasome-mediated degradation. J Biol Chem 276, 993-8
11035045   Curated Info

25

Vazquez F, Ramaswamy S, Nakamura N, Sellers WR (2000) Phosphorylation of the PTEN tail regulates protein stability and function. Mol Cell Biol 20, 5010-8
10866658   Curated Info