Ser21
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Home > Phosphorylation Site Page: > Ser21  -  C/EBP-alpha (human)

Site Information
PMSSHLQsPPHAPSS   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448308

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 5 ) , flow cytometry ( 2 ) , mutation of modification site ( 2 , 3 , 4 , 5 ) , peptide sequencing ( 5 ) , phospho-antibody ( 1 , 3 , 4 , 5 ) , western blotting ( 1 , 2 , 3 , 4 , 5 )
Disease tissue studied:
leukemia ( 1 , 2 , 4 ) , acute myelogenous leukemia ( 1 , 4 ) , chronic myelogenous leukemia ( 2 ) , liver cancer ( 3 ) , diabetes mellitus ( 3 ) , type 2 diabetes ( 3 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Regulatory protein:
CD34 (human) ( 2 ) , FUT4 (human) ( 2 ) , TfR (human) ( 2 )
Putative in vivo kinases:
ERK1 (human) ( 5 ) , ERK2 (human) ( 5 )
Kinases, in vitro:
ERK1 (human) ( 5 ) , ERK2 (human) ( 5 )
Treatments:
AG1296 ( 4 ) , butyrate ( 2 ) , FL ( 4 ) , hemin ( 2 ) , imatinib ( 4 ) , insulin ( 3 , 5 ) , IRC-084864 ( 1 ) , MLN-518 ( 4 ) , PD98059 ( 4 , 5 ) , PDGF ( 5 ) , phorbol_ester ( 5 ) , serum ( 5 ) , U0126 ( 5 )

Downstream Regulation
Effects of modification on C/EBP-alpha:
activity, induced ( 3 ) , activity, inhibited ( 4 , 5 ) , molecular association, regulation ( 2 ) , protein conformation ( 5 )
Effects of modification on biological processes:
cell differentiation, altered ( 2 , 4 ) , cell differentiation, inhibited ( 1 ) , transcription, altered ( 3 ) , transcription, inhibited ( 2 )
Inhibit interaction with:
DNA ( 2 )

References 

1

Bertoli S, et al. (2015) CDC25A governs proliferation and differentiation of FLT3-ITD acute myeloid leukemia. Oncotarget 6, 38061-78
26515730   Curated Info

2

Twu YC, et al. (2010) Phosphorylation status of transcription factor C/EBPalpha determines cell-surface poly-LacNAc branching (I antigen) formation in erythropoiesis and granulopoiesis. Blood 115, 2491-9
20101026   Curated Info

3

Qiao L, MacDougald OA, Shao J (2006) CCAAT/enhancer-binding protein alpha mediates induction of hepatic phosphoenolpyruvate carboxykinase by p38 mitogen-activated protein kinase. J Biol Chem 281, 24390-7
16807249   Curated Info

4

Radomska HS, et al. (2006) Block of C/EBP alpha function by phosphorylation in acute myeloid leukemia with FLT3 activating mutations. J Exp Med 203, 371-81
16446383   Curated Info

5

Ross SE, et al. (2004) Phosphorylation of C/EBPalpha inhibits granulopoiesis. Mol Cell Biol 24, 675-86
14701740   Curated Info