Ser79
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Home > Phosphorylation Site Page: > Ser79  -  ACC1 (rat)

Site Information
FHMRSsMsGLHLVKQ   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448292

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 23 , 26 ) , immunoprecipitation ( 23 ) , mass spectrometry ( 1 , 2 , 3 , 6 , 17 ) , peptide sequencing ( 26 ) , phospho-antibody ( 4 , 5 , 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 , 18 , 19 , 20 , 21 , 22 , 23 ) , phosphopeptide mapping ( 26 ) , western blotting ( 4 , 5 , 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 , 19 , 22 )
Disease tissue studied:
breast cancer ( 11 ) , liver cancer ( 11 , 22 )
Relevant cell line - cell type - tissue:
'muscle, skeletal' ( 5 , 7 , 10 , 15 , 21 , 23 ) , adipocyte-adipose tissue ( 26 ) , adipose tissue ( 3 , 9 , 13 ) , granulosa ( 18 ) , H4IIe (hepatic) ( 22 ) , heart ( 12 ) , hepatocyte ( 4 ) , hepatocyte-liver ( 19 ) , IEC-6 (epithelial) ( 1 ) , intestine ( 3 ) , liver ( 3 , 6 , 11 , 17 ) , lung ( 3 ) , mammary gland ( 11 ) , myocyte-heart ( 8 , 14 , 16 , 20 ) , PC-12 (chromaffin) [TrkA (rat), transfection] ( 2 ) , PC-12 (chromaffin) ( 2 ) , spleen ( 3 ) , stomach ( 3 ) , testis ( 3 ) , thymus ( 3 )

Upstream Regulation
Regulatory protein:
Akt1 (rat) ( 16 ) , CAB39 (human) ( 14 ) , LKB1 (human) ( 16 ) , LKB1 (mouse) ( 14 ) , STRAD (human) ( 14 )
Putative in vivo kinases:
AMPKA1 (rat) ( 26 )
Kinases, in vitro:
AMPKA1 (rat) ( 23 , 24 , 26 ) , AMPKA2 (rat) ( 25 , 27 )
Treatments:
acadesine ( 7 , 15 , 18 , 19 , 20 ) , adiponectin ( 21 ) , angiotensin_2 ( 8 ) , berberine ( 7 ) , caffeine ( 5 ) , calyculin_A ( 19 ) , cAMP_analog ( 26 ) , cantharidin ( 19 ) , clozapine ( 4 ) , colforsin ( 26 ) , compound_C ( 9 , 13 ) , DNP ( 7 ) , electrical_stimulation ( 23 ) , exercise ( 13 , 23 ) , food deprivation ( 11 ) , hypoxia ( 16 ) , IBMX ( 26 ) , Intralipid ( 20 ) , isoproterenol ( 26 ) , L-NAME ( 20 ) , liothyronine ( 8 , 10 ) , metformin ( 7 , 12 , 22 ) , microcystin-LR ( 19 ) , naringin ( 19 ) , okadaic_acid ( 19 ) , olanzapine ( 4 ) , oligomycin ( 22 ) , phenformin ( 12 ) , phentolamine ( 13 ) , phenylephrine ( 26 ) , prazosin ( 9 ) , propranolol ( 9 , 13 ) , racepinefrine ( 13 , 26 )

Downstream Regulation
Effects of modification on ACC1:
enzymatic activity, inhibited ( 23 , 24 , 25 , 26 )

References 

1

Courcelles M, et al. (2013) Phosphoproteome dynamics reveal novel ERK1/2 MAP kinase substrates with broad spectrum of functions. Mol Syst Biol 9, 669
23712012   Curated Info

2

Biarc J, Chalkley RJ, Burlingame AL, Bradshaw RA (2012) The induction of serine/threonine protein phosphorylations by a PDGFR/TrkA chimera in stably transfected PC12 cells. Mol Cell Proteomics 11, 15-30
22027198   Curated Info

3

Lundby A, et al. (2012) Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues. Nat Commun 3, 876
22673903   Curated Info

4

Oh KJ, et al. (2011) Atypical antipsychotic drugs perturb AMPK-dependent regulation of hepatic lipid metabolism. Am J Physiol Endocrinol Metab 300, E624-32
21224484   Curated Info

5

Egawa T, et al. (2011) Caffeine activates preferentially α1-isoform of 5'AMP-activated protein kinase in rat skeletal muscle. Acta Physiol (Oxf) 201, 227-38
21241457   Curated Info

6

Demirkan G, et al. (2011) Phosphoproteomic Profiling of In Vivo Signaling in Liver by the Mammalian Target of Rapamycin Complex 1 (mTORC1). PLoS One 6, e21729
21738781   Curated Info

7

Ma X, et al. (2010) Berberine-induced activation of 5'-adenosine monophosphate-activated protein kinase and glucose transport in rat skeletal muscles. Metabolism 59, 1619-27
20423742   Curated Info

8

Jiang SY, et al. (2010) A distinct AMP-activated protein kinase phosphorylation site characterizes cardiac hypertrophy induced by L-thyroxine and angiotensin II. Clin Exp Pharmacol Physiol 37, 919-25
20497424   Curated Info

9

Hattori A, et al. (2010) Beta-adrenergic-AMPK pathway phosphorylates acetyl-CoA carboxylase in a high-epinephrine rat model, SPORTS. Obesity (Silver Spring) 18, 48-54
19444233   Curated Info

10

de Lange P, et al. (2008) Rapid activation by 3,5,3'-L-triiodothyronine of adenosine 5'-monophosphate-activated protein kinase/acetyl-coenzyme a carboxylase and akt/protein kinase B signaling pathways: relation to changes in fuel metabolism and myosin heavy-chain protein content in rat gastrocnemius muscle in vivo. Endocrinology 149, 6462-70
18703632   Curated Info

11

Jiang W, Zhu Z, Thompson HJ (2008) Dietary energy restriction modulates the activity of AMP-activated protein kinase, Akt, and mammalian target of rapamycin in mammary carcinomas, mammary gland, and liver. Cancer Res 68, 5492-9
18593953   Curated Info

12

Zhang L, He H, Balschi JA (2007) Metformin and phenformin activate AMP-activated protein kinase in the heart by increasing cytosolic AMP concentration. Am J Physiol Heart Circ Physiol 293, H457-66
17369473   Curated Info

13

Koh HJ, et al. (2007) Adrenaline is a critical mediator of acute exercise-induced AMP-activated protein kinase activation in adipocytes. Biochem J 403, 473-81
17253964   Curated Info

14

Noga AA, et al. (2007) Expression of an active LKB1 complex in cardiac myocytes results in decreased protein synthesis associated with phenylephrine-induced hypertrophy. Am J Physiol Heart Circ Physiol 292, H1460-9
17098823   Curated Info

15

Fediuc S, Gaidhu MP, Ceddia RB (2006) Inhibition of insulin-stimulated glycogen synthesis by 5-aminoimidasole-4-carboxamide-1-beta-d-ribofuranoside-induced adenosine 5'-monophosphate-activated protein kinase activation: interactions with Akt, glycogen synthase kinase 3-3alpha/beta, and glycogen synthase in isolated rat soleus muscle. Endocrinology 147, 5170-7
16873531   Curated Info

16

Soltys CL, Kovacic S, Dyck JR (2006) Activation of cardiac AMP-activated protein kinase by LKB1 expression or chemical hypoxia is blunted by increased Akt activity. Am J Physiol Heart Circ Physiol 290, H2472-9
16428351   Curated Info

17

Moser K, White FM (2006) Phosphoproteomic analysis of rat liver by high capacity IMAC and LC-MS/MS. J Proteome Res 5, 98-104
16396499   Curated Info

18

Tosca L, et al. (2005) Adenosine 5'-monophosphate-activated protein kinase regulates progesterone secretion in rat granulosa cells. Endocrinology 146, 4500-13
16020477   Curated Info

19

Samari HR, et al. (2005) Stimulation of hepatocytic AMP-activated protein kinase by okadaic acid and other autophagy-suppressive toxins. Biochem J 386, 237-44
15461583   Curated Info

20

Shearer J, et al. (2004) AMPK stimulation increases LCFA but not glucose clearance in cardiac muscle in vivo. Am J Physiol Endocrinol Metab 287, E871-7
15265760   Curated Info

21

Tomas E, et al. (2002) Enhanced muscle fat oxidation and glucose transport by ACRP30 globular domain: acetyl-CoA carboxylase inhibition and AMP-activated protein kinase activation. Proc Natl Acad Sci U S A 99, 16309-13
12456889   Curated Info

22

Hawley SA, Gadalla AE, Olsen GS, Hardie DG (2002) The antidiabetic drug metformin activates the AMP-activated protein kinase cascade via an adenine nucleotide-independent mechanism. Diabetes 51, 2420-5
12145153   Curated Info

23

Park SH, et al. (2002) Phosphorylation-activity relationships of AMPK and acetyl-CoA carboxylase in muscle. J Appl Physiol 92, 2475-82
12015362   Curated Info

24

Ha J, Daniel S, Broyles SS, Kim KH (1994) Critical phosphorylation sites for acetyl-CoA carboxylase activity. J Biol Chem 269, 22162-8
7915280   Curated Info

25

Davies SP, Sim AT, Hardie DG (1990) Location and function of three sites phosphorylated on rat acetyl-CoA carboxylase by the AMP-activated protein kinase. Eur J Biochem 187, 183-90
1967580   Curated Info

26

Haystead TA, Moore F, Cohen P, Hardie DG (1990) Roles of the AMP-activated and cyclic-AMP-dependent protein kinases in the adrenaline-induced inactivation of acetyl-CoA carboxylase in rat adipocytes. Eur J Biochem 187, 199-205
1688796   Curated Info

27

Munday MR, Campbell DG, Carling D, Hardie DG (1988) Identification by amino acid sequencing of three major regulatory phosphorylation sites on rat acetyl-CoA carboxylase. Eur J Biochem 175, 331-8
2900138   Curated Info