Thr228

Site Information
DMADtCGtICHAADk SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 34551795

In vivo Characterization
Methods used to characterize site in vivo:	immunoassay ( 1 ) , mass spectrometry ( 2 ) , mass spectrometry (in vitro) ( 1 ) , mutation of modification site ( 1 ) , phospho-antibody ( 1 ) , western blotting ( 1 )
Disease tissue studied:	bone cancer ( 1 ) , melanoma skin cancer ( 1 )
Relevant cell line - cell type - tissue:	FHs 74 Int ( 1 ) , HOK (endothelial) ( 1 ) , HUVEC (endothelial) ( 1 ) , Jurkat (T lymphocyte) ( 2 ) , Saos-2 (bone cell) ( 1 ) , SK-MEL5 (melanocyte) ( 1 )

Upstream Regulation
Regulatory protein:	cGAS (human) ( 1 ) , ENPP1 (human) ( 1 ) , IKKE (human) ( 1 ) , Nik (human) ( 1 ) , STING (human) ( 1 ) , TREX1 (human) ( 1 )
Putative in vivo kinases:	TBK1 (human) ( 1 )
Kinases, in vitro:	TBK1 (human) ( 1 )
Treatments:	ADP ( 1 ) , AMP ( 1 ) , BX795 ( 1 ) , C-170 ( 1 ) , cGAMP ( 1 ) , cGMP_analog ( 1 ) , GMP ( 1 ) , H-151 ( 1 ) , IMP ( 1 ) , ionizing_radiation ( 1 ) , MMS ( 1 ) , siRNA ( 1 ) , UVC ( 1 )

Downstream Regulation
Effects of modification on PRPS1:	enzymatic activity, induced ( 1 ) , molecular association, regulation ( 1 )
Effects of modification on biological processes:	cell growth, induced ( 1 ) , DNA repair, induced ( 1 )

References
1	Liu R, et al. (2021) Innate immune response orchestrates phosphoribosyl pyrophosphate synthetases to support DNA repair. Cell Metab 34343500 Curated Info
2	Mulhern D (2011) CST Curation Set: 13265; Year: 2011; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]Q Antibodies Used to Purify Peptides prior to LCMS: Phospho-(Ser/Thr) ATM/ATR Substrate (S/TQG) (P-S/T2-100) Rabbit mAb Cat#: 6966, PTMScan(R) Phospho-ATM/ATR Substrate Motif (S/TQG) Immunoaffinity Beads Cat#: 6969 Curated Info

References

Liu R, et al. (2021) Innate immune response orchestrates phosphoribosyl pyrophosphate synthetases to support DNA repair. Cell Metab
34343500 Curated Info

Mulhern D (2011) CST Curation Set: 13265; Year: 2011; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]Q Antibodies Used to Purify Peptides prior to LCMS: Phospho-(Ser/Thr) ATM/ATR Substrate (S*/T*QG) (P-S/T2-100) Rabbit mAb Cat#: 6966, PTMScan(R) Phospho-ATM/ATR Substrate Motif (S*/T*QG) Immunoaffinity Beads Cat#: 6969
Curated Info