Ser281
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Home > Phosphorylation Site Page: > Ser281  -  MLK3 (human)

Site Information
WHKtTQMsAAGTYAW   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448262

In vivo Characterization
Methods used to characterize site in vivo:
electrophoretic mobility shift ( 1 ) , immunoprecipitation ( 2 ) , mutation of modification site ( 2 , 4 ) , phospho-antibody ( 1 , 2 , 3 ) , western blotting ( 1 , 2 , 3 )
Disease tissue studied:
lung cancer ( 2 )
Relevant cell line - cell type - tissue:
'brain, hippocampus' ( 3 ) , 'stem, embryonic' [MLK3 (mouse), genetic knockin] ( 1 ) , 293 (epithelial) ( 2 , 4 ) , A549 (pulmonary) ( 2 ) , HEK293T (epithelial) ( 1 ) , HeLa (cervical) ( 4 ) , osteoblast-calvarium ( 1 )

Upstream Regulation
Kinases, in vitro:
HPK1 (human) ( 4 ) , MLK3 (human) ( 4 )
Treatments:
carbachol ( 2 ) , ischemia/reperfusion ( 3 ) , vanadate ( 3 )

Downstream Regulation
Effects of modification on MLK3:
enzymatic activity, induced ( 4 ) , molecular association, regulation ( 2 )
Effects of modification on biological processes:
cell differentiation, induced ( 1 )
Induce interaction with:
GEFT (human) ( 2 )

References 

1

Zou W, et al. (2011) MLK3 regulates bone development downstream of the faciogenital dysplasia protein FGD1 in mice. J Clin Invest 121, 4383-92
21965325   Curated Info

2

Swenson-Fields KI, et al. (2008) MLK3 limits activated Galphaq signaling to Rho by binding to p63RhoGEF. Mol Cell 32, 43-56
18851832   Curated Info

3

Zhang QG, et al. (2006) Akt inhibits MLK3/JNK3 signaling by inactivating Rac1: a protective mechanism against ischemic brain injury. J Neurochem 98, 1886-98
16831194   Curated Info

4

Leung IW, Lassam N (2001) The kinase activation loop is the key to mixed lineage kinase-3 activation via both autophosphorylation and hematopoietic progenitor kinase 1 phosphorylation. J Biol Chem 276, 1961-7
11053428   Curated Info