Ser1621
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Home > Phosphorylation Site Page: > Ser1621  -  POLR2A (human)

Site Information
SysPtsPsYSPTSPS   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 3439802

In vivo Characterization
Methods used to characterize site in vivo:
electrophoretic mobility shift ( 11 ) , immunoprecipitation ( 6 , 8 , 11 ) , mass spectrometry ( 1 , 4 ) , mutation of modification site ( 6 , 10 , 11 ) , peptide sequencing ( 11 ) , phospho-antibody ( 1 , 2 , 3 , 5 , 6 , 9 , 10 ) , western blotting ( 1 , 2 , 3 , 5 , 6 , 9 , 11 )
Disease tissue studied:
colorectal cancer ( 2 ) , colorectal carcinoma ( 2 ) , lymphoma ( 1 , 9 , 11 ) , Burkitt's lymphoma ( 1 , 9 , 11 ) , ovarian cancer ( 5 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Kinases, in vitro:
CDK7 (human) ( 8 , 9 ) , CDK9 (human) ( 2 , 3 , 4 , 8 ) , DNAPK (human) ( 7 )
Treatments:
alvocidib ( 2 ) , CdkI-83 ( 5 ) , flavopiridol ( 1 ) , seliciclib ( 8 )

Downstream Regulation
Effects of modification on POLR2A:
molecular association, regulation ( 6 , 7 ) , phosphorylation ( 10 )
Effects of modification on biological processes:
transcription, altered ( 8 ) , transcription, induced ( 10 , 11 )
Induce interaction with:
INTS11 (human) ( 7 ) , POLR1B (human) ( 6 )

References 

1

Schüller R, et al. (2016) Heptad-Specific Phosphorylation of RNA Polymerase II CTD. Mol Cell 61, 305-14
26799765   Curated Info

2

Greifenberg AK, et al. (2016) Structural and Functional Analysis of the Cdk13/Cyclin K Complex. Cell Rep 14, 320-31
26748711   Curated Info

3

Liang K, et al. (2015) Characterization of Human Cyclin-Dependent Kinase 12 (CDK12) and CDK13 Complexes in C-Terminal Domain Phosphorylation, Gene Transcription, and RNA Processing. Mol Cell Biol 35, 928-38
25561469   Curated Info

4

Bösken CA, et al. (2014) The structure and substrate specificity of human Cdk12/Cyclin K. Nat Commun 5, 3505
24662513   Curated Info

5

Liu X, et al. (2012) In vitro antitumor mechanism of a novel cyclin-dependent kinase inhibitor CDKI-83. Invest New Drugs 30, 889-97
21331744   Curated Info

6

Egloff S, et al. (2012) Ser7 phosphorylation of the CTD recruits the RPAP2 Ser5 phosphatase to snRNA genes. Mol Cell 45, 111-22
22137580   Curated Info

7

Egloff S, et al. (2010) The integrator complex recognizes a new double mark on the RNA polymerase II carboxyl-terminal domain. J Biol Chem 285, 20564-9
20457598   Curated Info

8

Boeing S, et al. (2010) RNA polymerase II C-terminal heptarepeat domain Ser-7 phosphorylation is established in a mediator-dependent fashion. J Biol Chem 285, 188-96
19901026   Curated Info

9

Akhtar MS, et al. (2009) TFIIH kinase places bivalent marks on the carboxy-terminal domain of RNA polymerase II. Mol Cell 34, 387-93
19450536   Curated Info

10

Egloff S, et al. (2007) Serine-7 of the RNA polymerase II CTD is specifically required for snRNA gene expression. Science 318, 1777-9
18079403   Curated Info

11

Chapman RD, et al. (2007) Transcribing RNA polymerase II is phosphorylated at CTD residue serine-7. Science 318, 1780-2
18079404   Curated Info