Tyr248
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Home > Phosphorylation Site Page: > Tyr248  -  GTF2I (human)

Site Information
EESEDPDyyQYNIQA   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448250
Associated spectra:  1 CST

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 4 ) , mass spectrometry ( 2 , 3 ) , mutation of modification site ( 1 , 4 , 5 , 6 ) , phospho-antibody ( 5 , 6 ) , western blotting ( 5 , 6 )
Relevant cell line - cell type - tissue:
3T3 (fibroblast) [SHP-2 (mouse), homozygous knockout] ( 5 ) , 3T3 (fibroblast) ( 1 ) , COS (fibroblast) ( 4 , 6 ) , HEK293T (epithelial) ( 5 ) , Jurkat (T lymphocyte) ( 2 , 3 ) , Swiss 3T3 (fibroblast) ( 5 )

Upstream Regulation
Putative in vivo kinases:
Src (human) ( 1 )
Treatments:
genistein ( 5 ) , thapsigargin ( 5 )

Downstream Regulation
Effects of modification on GTF2I:
activity, induced ( 5 , 6 ) , intracellular localization ( 5 )
Effects of modification on biological processes:
transcription, altered ( 1 , 5 , 6 ) , transcription, induced ( 4 )

References 

1

Bu Y, Gao L, Gelman IH (2011) Role for transcription factor TFII-I in the suppression of SSeCKS/Gravin/Akap12 transcription by Src. Int J Cancer 128, 1836-42
20568114   Curated Info

2

Guo A (2010) CST Curation Set: 9182; Year: 2010; Biosample/Treatment: cell line, Jurkat/pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

3

Guo A (2010) CST Curation Set: 9181; Year: 2010; Biosample/Treatment: cell line, Jurkat/pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

4

Rajaiya J, et al. (2006) Induction of immunoglobulin heavy-chain transcription through the transcription factor Bright requires TFII-I. Mol Cell Biol 26, 4758-68
16738337   Curated Info

5

Hong M, et al. (2005) Transcriptional regulation of the Grp78 promoter by endoplasmic reticulum stress: role of TFII-I and its tyrosine phosphorylation. J Biol Chem 280, 16821-8
15664986   Curated Info

6

Novina CD, Cheriyath V, Roy AL (1998) Regulation of TFII-I activity by phosphorylation. J Biol Chem 273, 33443-8
9837922   Curated Info

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