Thr453
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Home > Phosphorylation Site Page: > Thr453  -  SP1 (human)

Site Information
SGPIIIRtPTVGPNG   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448226

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 3 ) , electrophoretic mobility shift ( 3 , 4 ) , immunoprecipitation ( 2 ) , mutation of modification site ( 3 , 5 ) , phospho-antibody ( 1 , 2 , 4 , 5 ) , western blotting ( 1 , 2 )
Relevant cell line - cell type - tissue:
B104-1-1 (fibroblast) ( 2 ) , Dede (fibroblast) ( 5 ) , HaCaT (keratinocyte) ( 4 ) , SL2 ( 5 ) , VSMC [TRAIL (mouse), homozygous knockout] ( 1 ) , WKY ('muscle, smooth') ( 3 )

Upstream Regulation
Regulatory protein:
TRAIL (mouse) ( 1 )
Putative in vivo kinases:
ERK1 (rat) ( 3 , 4 ) , ERK2 (human) ( 3 , 5 )
Kinases, in vitro:
ERK2 (human) ( 5 )
Phosphatases, in vitro:
PPP2CB (human) ( 4 )
Treatments:
FGF2 ( 3 ) , injury ( 1 ) , PD98059 ( 3 , 4 ) , perifosine ( 4 ) , SU5402 ( 1 )

Downstream Regulation
Effects of modification on SP1:
molecular association, regulation ( 1 )
Effects of modification on biological processes:
transcription, altered ( 1 , 5 ) , transcription, induced ( 4 ) , transcription, inhibited ( 3 )
Induce interaction with:
NFkB-p65 (human) ( 1 )

References 

1

Chan J, et al. (2010) TRAIL promotes VSMC proliferation and neointima formation in a FGF-2-, Sp1 phosphorylation-, and NFkappaB-dependent manner. Circ Res 106, 1061-71
20150555   Curated Info

2

Hsu MC, Chang HC, Hung WC (2006) HER-2/neu represses the metastasis suppressor RECK via ERK and Sp transcription factors to promote cell invasion. J Biol Chem 281, 4718-25
16377629   Curated Info

3

Bonello MR, Khachigian LM (2004) Fibroblast growth factor-2 represses platelet-derived growth factor receptor-alpha (PDGFR-alpha) transcription via ERK1/2-dependent Sp1 phosphorylation and an atypical cis-acting element in the proximal PDGFR-alpha promoter. J Biol Chem 279, 2377-82
14593115   Curated Info

4

De Siervi A, et al. (2004) Transcriptional activation of p21(waf1/cip1) by alkylphospholipids: role of the mitogen-activated protein kinase pathway in the transactivation of the human p21(waf1/cip1) promoter by Sp1. Cancer Res 64, 743-50
14744793   Curated Info

5

Milanini-Mongiat J, Pouysségur J, Pagès G (2002) Identification of two Sp1 phosphorylation sites for p42/p44 mitogen-activated protein kinases: their implication in vascular endothelial growth factor gene transcription. J Biol Chem 277, 20631-9
11904305   Curated Info