Thr1062
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage PhosphoSitePlus®
Powered by Cell Signaling Technology
Home > Phosphorylation Site Page: > Thr1062  -  TOPBP1 (human)

Site Information
NDSkGVLtQtLEMRE   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 460941

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 1 ) , mass spectrometry ( 2 , 3 , 4 ) , mutation of modification site ( 1 ) , phosphoamino acid analysis ( 1 ) , western blotting ( 1 )
Disease tissue studied:
brain cancer ( 4 ) , glioblastoma ( 4 ) , glioma ( 4 ) , colorectal cancer ( 1 ) , colorectal carcinoma ( 1 )
Relevant cell line - cell type - tissue:
293T (epithelial) ( 3 ) , HCT116 (intestinal) ( 1 ) , HeLa (cervical) ( 2 ) , M059J (glial) ( 4 )

Upstream Regulation
Regulatory protein:
ATR (human) ( 1 )
Kinases, in vitro:
ATM (human) ( 5 )
Treatments:
ionizing_radiation ( 3 ) , UV ( 3 )

References 

1

Liu S, et al. (2011) ATR Autophosphorylation as a Molecular Switch for Checkpoint Activation. Mol Cell 43, 192-202
21777809   Curated Info

2

Zhou J (2009) CST Curation Set: 6246; Year: 2009; Biosample/Treatment: cell line, HeLa/UV; Disease: cervical adenocarcinoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]Q Antibodies Used to Purify Peptides prior to LCMS: Phospho(Ser/Thr) ATM/ATR Substrate Antibody (polyAB) Cat#: 3005, PTMScan(R) Phospho-ATM/ATR Substrate Motif (pS/pTQ) Immunoaffinity Beads Cat#: 1980
Curated Info

3

Matsuoka S, et al. (2007) ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage. Science 316, 1160-6
17525332   Curated Info

4

Stokes M (2005) CST Curation Set: 908; Year: 2005; Biosample/Treatment: cell line, M059J/UV; Disease: glioblastoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]Q Antibodies Used to Purify Peptides prior to LCMS: Phospho-(Ser/Thr) ATM/ATR Substrate Antibody Cat#: 2851
Curated Info

5

Yamane K, Wu X, Chen J (2002) A DNA damage-regulated BRCT-containing protein, TopBP1, is required for cell survival. Mol Cell Biol 22, 555-66
11756551   Curated Info