Ser12
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage PhosphoSitePlus® v6.7.4
Powered by Cell Signaling Technology
Home > Phosphorylation Site Page: > Ser12  -  Myb (human)

Site Information
PRHSIYssDEDDEDF   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448187

In vivo Characterization
Methods used to characterize site in vivo:
mass spectrometry ( 1 , 2 , 3 ) , mutation of modification site ( 5 , 6 ) , phospho-antibody ( 5 ) , western blotting ( 5 )
Disease tissue studied:
adrenal cancer ( 6 ) , pheochromocytoma ( 6 ) , lymphoma ( 5 ) , anaplastic large cell lymphoma ( 5 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Kinases, in vitro:
CK2A1 (human) ( 4 )

Downstream Regulation
Effects of modification on Myb:
molecular association, regulation ( 6 )
Effects of modification on biological processes:
transcription, altered ( 6 )
Inhibit interaction with:
DNA ( 6 )

References 

1

Mertins P, et al. (2013) Integrated proteomic analysis of post-translational modifications by serial enrichment. Nat Methods 10, 634-7
23749302   Curated Info

2

Mulhern D (2011) CST Curation Set: 12679; Year: 2011; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: (F/Y/M)Xp[ST](L/I/M)
Curated Info

3

Possemato A (2009) CST Curation Set: 6911; Year: 2009; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-Akt Substrate (RXRXXS*/T*) (23C8D2) Rabbit mAb Cat#: 10001, PTMScan(R) Phospho-Akt Substrate Motif (RXRXXS*/T*) Immunoaffinity Beads Cat#: 1979
Curated Info

4

Bergholtz S, et al. (2001) The highly conserved DNA-binding domains of A-, B- and c-Myb differ with respect to DNA-binding, phosphorylation and redox properties. Nucleic Acids Res 29, 3546-56
11522824   Curated Info

5

Cures A, et al. (2001) Constitutive c-Myb amino-terminal phosphorylation and DNA binding activity uncoupled during entry and passage through the cell cycle. Oncogene 20, 1784-92
11313925   Curated Info

6

Bousset K, et al. (1994) Regulation of transcription factors c-Myc, Max, and c-Myb by casein kinase II. Cell Mol Biol Res 40, 501-11
7735324   Curated Info