Ser269
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Home > Phosphorylation Site Page: > Ser269  -  AQP2 (rat)

Site Information
PQsLPRGskA_____   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 3339002

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 4 ) , mass spectrometry ( 20 , 21 ) , mutation of modification site ( 16 ) , phospho-antibody ( 3 , 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 , 15 , 18 , 19 , 20 ) , western blotting ( 3 , 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 13 , 15 , 18 , 19 )
Relevant cell line - cell type - tissue:
'kidney, inner medulla' ( 6 , 7 , 8 , 10 , 13 , 18 , 19 , 20 , 21 ) , IMCD ( 15 ) , kidney ( 12 , 19 ) , LLC-PK1 (renal) ( 3 , 16 ) , MDCK (epithelial) ( 4 , 5 , 9 , 12 ) , mpkCCD (renal) ( 18 )

Upstream Regulation
Treatments:
4AD ( 15 ) , butaprost ( 9 , 17 ) , calyculin_A ( 7 , 8 ) , cAMP_analog ( 20 ) , CAY10580 ( 9 ) , colforsin ( 5 , 15 ) , desmopressin ( 6 , 11 , 18 , 19 , 20 , 21 ) , FK506 ( 7 , 8 ) , H-89 ( 20 ) , IBMX ( 6 ) , ibuprofen ( 13 ) , meloxicam ( 13 ) , Mn(2+) ( 3 ) , NKH_477 ( 4 ) , PGE2 ( 17 ) , roflumilast ( 6 ) , roflumilast N-oxide ( 6 ) , sulprostone ( 10 ) , tolvaptan ( 14 ) , vasopressin ( 3 , 12 , 14 )

Downstream Regulation
Effects of modification on AQP2:
intracellular localization ( 12 , 18 , 19 )
Effects of modification on biological processes:
endocytosis, inhibited ( 15 , 16 )

References 

1

Masuda T, et al. (2020) Osmotic diuresis by SGLT2 inhibition stimulates vasopressin-induced water reabsorption to maintain body fluid volume. Physiol Rep 8, e14360
31994353   Curated Info

2

Cheung PW, et al. (2018) Inhibition of non-receptor tyrosine kinase Src induces phosphoserine 256-independent aquaporin-2 membrane accumulation. J Physiol
30488437   Curated Info

3

Lei L, et al. (2017) Manganese promotes intracellular accumulation of AQP2 via modulating F-actin polymerization and reduces urinary concentration in mice. Am J Physiol Renal Physiol, ajprenal003912017
29046300   Curated Info

4

Yui N, Ando F, Sasaki S, Uchida S (2017) Ser-261 phospho-regulation is involved in pS256 and pS269-mediated aquaporin-2 apical translocation. Biochem Biophys Res Commun 490, 1039-1044
28668390   Curated Info

5

Yui N, Sasaki S, Uchida S (2017) Aquaporin-2 Ser-261 phosphorylation is regulated in combination with Ser-256 and Ser-269 phosphorylation. Biochem Biophys Res Commun 482, 524-529
27889609   Curated Info

6

Umejiego EN, Wang Y, Knepper MA, Chou CL (2017) Roflumilast and aquaporin-2 regulation in rat renal inner medullary collecting duct. Physiol Rep 5
28108651   Curated Info

7

Ren H, et al. (2016) Phosphatase inhibition increases AQP2 accumulation in the rat IMCD apical plasma membrane. Am J Physiol Renal Physiol 311, F1189-F1197
27488997   Curated Info

8

Al-Bataineh MM, et al. (2016) Activation of the metabolic sensor AMP-activated protein kinase inhibits aquaporin-2 function in kidney principal cells. Am J Physiol Renal Physiol 311, F890-F900
27534994   Curated Info

9

Olesen ET, et al. (2016) The vasopressin type 2 receptor and prostaglandin receptors EP2 and EP4 can increase aquaporin-2 plasma membrane targeting through a cAMP-independent pathway. Am J Physiol Renal Physiol 311, F935-F944
27558562   Curated Info

10

Hao S, et al. (2016) The EP3 receptor regulates water excretion in response to high salt intake. Am J Physiol Renal Physiol 311, F822-F829
27465993   Curated Info

11

Choi HJ, Jung HJ, Kwon TH (2015) Extracellular pH affects phosphorylation and intracellular trafficking of AQP2 in inner medullary collecting duct cells. Am J Physiol Renal Physiol 308, F737-48
25651562   Curated Info

12

Rice WL, et al. (2015) Polarized Trafficking of AQP2 Revealed in Three Dimensional Epithelial Culture. PLoS One 10, e0131719
26147297   Curated Info

13

Ren H, et al. (2015) NSAIDs Alter Phosphorylated Forms of AQP2 in the Inner Medullary Tip. PLoS One 10, e0141714
26517129   Curated Info

14

Miranda CA, Lee JW, Chou CL, Knepper MA (2014) Tolvaptan as a tool in renal physiology. Am J Physiol Renal Physiol 306, F359-66
24305472   Curated Info

15

Bogum J, et al. (2013) Small-molecule screening identifies modulators of aquaporin-2 trafficking. J Am Soc Nephrol 24, 744-58
23559583   Curated Info

16

Rice WL, et al. (2012) Differential, phosphorylation dependent trafficking of AQP2 in LLC-PK1 cells. PLoS One 7, e32843
22403603   Curated Info

17

Olesen ET, et al. (2011) Vasopressin-independent targeting of aquaporin-2 by selective E-prostanoid receptor agonists alleviates nephrogenic diabetes insipidus. Proc Natl Acad Sci U S A 108, 12949-54
21768374   Curated Info

18

Xie L, et al. (2010) Quantitative analysis of aquaporin-2 phosphorylation. Am J Physiol Renal Physiol 298, F1018-23
20089674   Curated Info

19

Moeller HB, Knepper MA, Fenton RA (2009) Serine 269 phosphorylated aquaporin-2 is targeted to the apical membrane of collecting duct principal cells. Kidney Int 75, 295-303
18843259   Curated Info

20

Hoffert JD, et al. (2008) Vasopressin-stimulated increase in phosphorylation at Ser269 potentiates plasma membrane retention of aquaporin-2. J Biol Chem 283, 24617-27
18606813   Curated Info

21

Hoffert JD, et al. (2006) Quantitative phosphoproteomics of vasopressin-sensitive renal cells: regulation of aquaporin-2 phosphorylation at two sites. Proc Natl Acad Sci U S A 103, 7159-64
16641100   Curated Info