Ser269
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage PhosphoSitePlus® v6.8.0
Powered by Cell Signaling Technology
Home > Phosphorylation Site Page: > Ser269  -  AQP2 (mouse)

Site Information
PQsLPRGskA_____   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 3339002

In vivo Characterization
Methods used to characterize site in vivo:
immunoassay ( 2 ) , immunoprecipitation ( 2 , 3 , 4 , 5 ) , mass spectrometry ( 12 ) , mutation of modification site ( 1 , 4 , 6 , 7 , 8 , 13 , 14 , 16 ) , phospho-antibody ( 2 , 3 , 4 , 5 , 6 , 7 , 9 , 10 , 11 , 14 , 15 , 16 ) , western blotting ( 2 , 3 , 4 , 5 , 6 , 8 , 9 , 10 , 11 , 13 , 14 )
Relevant cell line - cell type - tissue:
'kidney, inner medulla' ( 10 , 15 ) , Flp-In-T-REx-293 ( 1 ) , HEK293T (epithelial) ( 7 ) , kidney ( 16 ) , LLC-PK1 (renal) ( 6 ) , MDCK (epithelial) ( 8 , 9 , 13 , 16 ) , MDCK-FTM ( 1 ) , mpkCCD (renal) ( 2 , 3 , 4 , 7 , 11 , 12 ) , oocyte ( 14 )

Upstream Regulation
Regulatory protein:
ADCY6 (mouse) ( 10 ) , RAB5A (mouse) ( 2 ) , Rab7 (mouse) ( 2 )
Putative in vivo kinases:
ROCK1 (human) ( 1 )
Treatments:
8-CPT-2Me-cAMP ( 3 ) , dDAVP ( 2 , 3 ) , ddFSK ( 3 ) , desmopressin ( 4 , 5 , 7 , 10 ) , erlotinib ( 6 ) , methyl-beta-cyclodextrin ( 3 ) , oxytocin ( 9 ) , vasopressin ( 2 , 9 ) , Y27632 ( 1 )

Downstream Regulation
Effects of modification on AQP2:
intracellular localization ( 1 , 8 , 9 , 13 , 16 ) , molecular association, regulation ( 4 , 7 , 13 ) , protein stabilization ( 13 )
Effects of modification on biological processes:
endocytosis, altered ( 13 ) , endocytosis, induced ( 2 ) , endocytosis, inhibited ( 4 )
Induce interaction with:
14-3-3 theta (human) ( 7 ) , 14-3-3 zeta (human) ( 7 )
Inhibit interaction with:
HSP70 (human) ( 13 ) , SIPA1L1 (mouse) ( 4 )

References 

1

Venneri M, et al. (2023) Novel signalling pathways in nephrogenic syndrome of inappropriate antidiuresis: functional implication of site-specific AQP2 phosphorylation. J Physiol
36823952   Curated Info

2

Wong KY, et al. (2020) Intracellular location of aquaporin-2 serine 269 phosphorylation and dephosphorylation in kidney collecting duct cells. Am J Physiol Renal Physiol
32799672   Curated Info

3

Moeller HB, Fuglsang CH, Pedersen CN, Fenton RA (2018) Basolateral cholesterol depletion alters Aquaporin-2 post-translational modifications and disrupts apical plasma membrane targeting. Biochem Biophys Res Commun 495, 157-162
29103957   Curated Info

4

Wang PJ, et al. (2017) Vasopressin-induced serine 269 phosphorylation reduces Sipa1l1 (signal-induced proliferation-associated 1 like 1)-mediated aquaporin-2 endocytosis. J Biol Chem 292, 7984-7993
28336531   Curated Info

5

Yui N, Sasaki S, Uchida S (2017) Aquaporin-2 Ser-261 phosphorylation is regulated in combination with Ser-256 and Ser-269 phosphorylation. Biochem Biophys Res Commun 482, 524-529
27889609   Curated Info

6

Cheung PW, et al. (2016) EGF Receptor Inhibition by Erlotinib Increases Aquaporin 2-Mediated Renal Water Reabsorption. J Am Soc Nephrol 27, 3105-3116
27694161   Curated Info

7

Moeller HB, et al. (2016) Regulation of the Water Channel Aquaporin-2 via 14-3-3θ and -ζ. J Biol Chem 291, 2469-84
26645691   Curated Info

8

Moeller HB, et al. (2014) Phosphorylation and ubiquitylation are opposing processes that regulate endocytosis of the water channel aquaporin-2. J Cell Sci 127, 3174-83
24876223   Curated Info

9

Feinstein TN, et al. (2013) Noncanonical control of vasopressin receptor type 2 signaling by retromer and arrestin. J Biol Chem 288, 27849-60
23935101   Curated Info

10

Rieg T, et al. (2010) Adenylate cyclase 6 determines cAMP formation and aquaporin-2 phosphorylation and trafficking in inner medulla. J Am Soc Nephrol 21, 2059-68
20864687   Curated Info

11

Xie L, et al. (2010) Quantitative analysis of aquaporin-2 phosphorylation. Am J Physiol Renal Physiol 298, F1018-23
20089674   Curated Info

12

Rinschen MM, et al. (2010) Quantitative phosphoproteomic analysis reveals vasopressin V2-receptor-dependent signaling pathways in renal collecting duct cells. Proc Natl Acad Sci U S A 107, 3882-7
20139300   Curated Info

13

Moeller HB, Praetorius J, Rützler MR, Fenton RA (2010) Phosphorylation of aquaporin-2 regulates its endocytosis and protein-protein interactions. Proc Natl Acad Sci U S A 107, 424-9
19966308   Curated Info

14

Moeller HB, Macaulay N, Knepper MA, Fenton RA (2009) Role of multiple phosphorylation sites in the COOH-terminal tail of aquaporin-2 for water transport: evidence against channel gating. Am J Physiol Renal Physiol 296, F649-57
19144687   Curated Info

15

Moeller HB, Knepper MA, Fenton RA (2009) Serine 269 phosphorylated aquaporin-2 is targeted to the apical membrane of collecting duct principal cells. Kidney Int 75, 295-303
18843259   Curated Info

16

Hoffert JD, et al. (2008) Vasopressin-stimulated increase in phosphorylation at Ser269 potentiates plasma membrane retention of aquaporin-2. J Biol Chem 283, 24617-27
18606813   Curated Info

Developed with grants from  NIH Logo and literature mining with Linguamatics  I2E Logo Creative Commons License PhosphoSite, created by Cell Signaling Technology is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License.

©2003-2024 Cell Signaling Technology, Inc.