Ser269
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Home > Phosphorylation Site Page: > Ser269  -  AQP2 (mouse)

Site Information
PQsLPRGskA_____   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 3339002

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 1 , 2 , 3 ) , mass spectrometry ( 10 ) , mutation of modification site ( 2 , 4 , 5 , 6 , 11 , 12 , 14 ) , phospho-antibody ( 1 , 2 , 3 , 4 , 5 , 7 , 8 , 9 , 12 , 13 , 14 ) , western blotting ( 1 , 2 , 3 , 4 , 6 , 7 , 8 , 9 , 11 , 12 )
Relevant cell line - cell type - tissue:
'kidney, inner medulla' ( 8 , 13 ) , HEK293T (epithelial) ( 5 ) , kidney ( 14 ) , LLC-PK1 (renal) ( 4 ) , MDCK (epithelial) ( 6 , 7 , 11 , 14 ) , mpkCCD (renal) ( 1 , 2 , 5 , 9 , 10 ) , oocyte ( 12 )

Upstream Regulation
Regulatory protein:
ADCY6 (mouse) ( 8 )
Treatments:
8-CPT-2Me-cAMP ( 1 ) , dDAVP ( 1 ) , ddFSK ( 1 ) , desmopressin ( 2 , 3 , 5 , 8 ) , erlotinib ( 4 ) , methyl-beta-cyclodextrin ( 1 ) , oxytocin ( 7 ) , vasopressin ( 7 )

Downstream Regulation
Effects of modification on AQP2:
intracellular localization ( 6 , 7 , 11 , 14 ) , molecular association, regulation ( 2 , 5 , 11 ) , protein stabilization ( 11 )
Effects of modification on biological processes:
endocytosis, altered ( 11 ) , endocytosis, inhibited ( 2 )
Induce interaction with:
14-3-3 theta (human) ( 5 ) , 14-3-3 zeta (human) ( 5 )
Inhibit interaction with:
HSP70 (human) ( 11 ) , SIPA1L1 (mouse) ( 2 )

References 

1

Moeller HB, Fuglsang CH, Pedersen CN, Fenton RA (2018) Basolateral cholesterol depletion alters Aquaporin-2 post-translational modifications and disrupts apical plasma membrane targeting. Biochem Biophys Res Commun 495, 157-162
29103957   Curated Info

2

Wang PJ, et al. (2017) Vasopressin-induced serine 269 phosphorylation reduces Sipa1l1 (signal-induced proliferation-associated 1 like 1)-mediated aquaporin-2 endocytosis. J Biol Chem 292, 7984-7993
28336531   Curated Info

3

Yui N, Sasaki S, Uchida S (2017) Aquaporin-2 Ser-261 phosphorylation is regulated in combination with Ser-256 and Ser-269 phosphorylation. Biochem Biophys Res Commun 482, 524-529
27889609   Curated Info

4

Cheung PW, et al. (2016) EGF Receptor Inhibition by Erlotinib Increases Aquaporin 2-Mediated Renal Water Reabsorption. J Am Soc Nephrol 27, 3105-3116
27694161   Curated Info

5

Moeller HB, et al. (2016) Regulation of the Water Channel Aquaporin-2 via 14-3-3θ and -ζ. J Biol Chem 291, 2469-84
26645691   Curated Info

6

Moeller HB, et al. (2014) Phosphorylation and ubiquitylation are opposing processes that regulate endocytosis of the water channel aquaporin-2. J Cell Sci 127, 3174-83
24876223   Curated Info

7

Feinstein TN, et al. (2013) Noncanonical control of vasopressin receptor type 2 signaling by retromer and arrestin. J Biol Chem 288, 27849-60
23935101   Curated Info

8

Rieg T, et al. (2010) Adenylate cyclase 6 determines cAMP formation and aquaporin-2 phosphorylation and trafficking in inner medulla. J Am Soc Nephrol 21, 2059-68
20864687   Curated Info

9

Xie L, et al. (2010) Quantitative analysis of aquaporin-2 phosphorylation. Am J Physiol Renal Physiol 298, F1018-23
20089674   Curated Info

10

Rinschen MM, et al. (2010) Quantitative phosphoproteomic analysis reveals vasopressin V2-receptor-dependent signaling pathways in renal collecting duct cells. Proc Natl Acad Sci U S A 107, 3882-7
20139300   Curated Info

11

Moeller HB, Praetorius J, Rützler MR, Fenton RA (2010) Phosphorylation of aquaporin-2 regulates its endocytosis and protein-protein interactions. Proc Natl Acad Sci U S A 107, 424-9
19966308   Curated Info

12

Moeller HB, Macaulay N, Knepper MA, Fenton RA (2009) Role of multiple phosphorylation sites in the COOH-terminal tail of aquaporin-2 for water transport: evidence against channel gating. Am J Physiol Renal Physiol 296, F649-57
19144687   Curated Info

13

Moeller HB, Knepper MA, Fenton RA (2009) Serine 269 phosphorylated aquaporin-2 is targeted to the apical membrane of collecting duct principal cells. Kidney Int 75, 295-303
18843259   Curated Info

14

Hoffert JD, et al. (2008) Vasopressin-stimulated increase in phosphorylation at Ser269 potentiates plasma membrane retention of aquaporin-2. J Biol Chem 283, 24617-27
18606813   Curated Info