Thr269
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Home > Phosphorylation Site Page: > Thr269  -  AQP2 (human)

Site Information
PQsLPRGtkA_____   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 3339002

In vivo Characterization
Methods used to characterize site in vivo:
electrophoretic mobility shift ( 5 ) , immunoassay ( 5 ) , immunoprecipitation ( 3 ) , mass spectrometry ( 1 , 4 ) , mutation of modification site ( 3 , 4 , 5 ) , phospho-antibody ( 1 , 2 , 3 , 5 ) , western blotting ( 1 , 2 , 3 , 4 , 5 )
Disease tissue studied:
leukemia ( 3 )
Relevant cell line - cell type - tissue:
E.coli (bacterial) ( 4 ) , M1 (myeloid) ( 3 ) , MCD4 (renal) ( 2 , 3 ) , MDCK (epithelial) ( 5 ) , mpkCCD (renal) ( 5 ) , Pichia pastoris (yeast cell) ( 4 ) , urine ( 1 )

Upstream Regulation
Regulatory protein:
AVPR2 (human) ( 2 ) , CHIP (human) ( 3 )
Putative in vivo kinases:
ROCK1 (human) ( 2 )
Kinases, in vitro:
ROCK1 (human) ( 2 )
Treatments:
NKH_477 ( 5 ) , vasopressin ( 1 , 5 ) , Y27632 ( 2 )

Downstream Regulation
Effects of modification on AQP2:
intracellular localization ( 2 , 5 ) , molecular association, regulation ( 4 )
Effects of modification on biological processes:
endocytosis, inhibited ( 1 )
Inhibit interaction with:
VTA1 (mouse) ( 4 )

References 

1

Sakai M, et al. (2020) Phosphorylation profile of human AQP2 in urinary exosomes by LC-MS/MS phosphoproteomic analysis. Clin Exp Nephrol
32529500   Curated Info

2

Ranieri M, et al. (2020) The Vasopressin Receptor 2 Mutant R137L Linked to the Nephrogenic Syndrome of Inappropriate Antidiuresis (NSIAD) Signals through an Alternative Pathway that Increases AQP2 Membrane Targeting Independently of S256 Phosphorylation. Cells 9
32486031   Curated Info

3

Centrone M, et al. (2017) AQP2 Abundance is Regulated by the E3-Ligase CHIP Via HSP70. Cell Physiol Biochem 44, 515-531
29145196   Curated Info

4

Roche JV, et al. (2017) Phosphorylation of human aquaporin 2 (AQP2) allosterically controls its interaction with the lysosomal trafficking protein LIP5. J Biol Chem 292, 14636-14648
28710278   Curated Info

5

Trimpert C, et al. (2012) Vasopressin increases S261 phosphorylation in AQP2-P262L, a mutant in recessive nephrogenic diabetes insipidus. Nephrol Dial Transplant 27, 4389-97
22778181   Curated Info

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