Ser602
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Home > Phosphorylation Site Page: > Ser602  -  eplin (mouse)

Site Information
FRTSsIKsPKAssPS   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 459750

In vivo Characterization
Methods used to characterize site in vivo:
electrophoretic mobility shift ( 10 ) , immunoassay ( 10 ) , immunoprecipitation ( 10 ) , mass spectrometry ( 2 , 3 , 4 , 5 , 6 , 8 , 9 , 10 ) , mass spectrometry (in vitro) ( 7 ) , mutation of modification site ( 10 ) , phospho-antibody ( 10 ) , western blotting ( 10 )
Relevant cell line - cell type - tissue:
'3T3-L1, differentiated' (adipocyte) ( 2 , 5 ) , 'fat, brown' ( 9 ) , 3T3 (fibroblast) [BRAF (human), transfection] ( 10 ) , 3T3 (fibroblast) [CDC42 (human), transfection] ( 4 ) , 3T3 (fibroblast) [KRas (human), transfection] ( 4 ) , 3T3 (fibroblast) ( 4 ) , C2C12 (myoblast) ( 7 ) , HEK293T (epithelial) ( 10 ) , HeLa (cervical) ( 10 ) , kidney ( 9 ) , liver ( 6 ) , liver [leptin (mouse), homozygous knockout] ( 6 ) , MEF (fibroblast) [TSC2 (mouse), homozygous knockout] ( 8 ) , stromal ( 3 )

Upstream Regulation
Regulatory protein:
PAK4 (mouse) ( 4 )
Putative in vivo kinases:
ERK1 (human) ( 10 )
Kinases, in vitro:
ERK1 (human) ( 10 )
Treatments:
4-HT ( 10 ) , CIAP ( 10 ) , insulin ( 5 ) , ischemia ( 3 ) , PDGF ( 10 ) , U0126 ( 10 )

Downstream Regulation
Effects of modification on eplin:
intracellular localization ( 10 ) , molecular association, regulation ( 10 )
Effects of modification on biological processes:
cell motility, induced ( 10 ) , cytoskeletal reorganization ( 10 )
Inhibit interaction with:
ACTB (human) ( 10 )

References 

1

Sacco F, et al. (2016) Glucose-regulated and drug-perturbed phosphoproteome reveals molecular mechanisms controlling insulin secretion. Nat Commun 7, 13250
27841257   Curated Info

2

Minard AY, et al. (2016) mTORC1 Is a Major Regulatory Node in the FGF21 Signaling Network in Adipocytes. Cell Rep 17, 29-36
27681418   Curated Info

3

Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704
24719451   Curated Info

4

Gnad F, et al. (2013) Systems-wide Analysis of K-Ras, Cdc42, and PAK4 Signaling by Quantitative Phosphoproteomics. Mol Cell Proteomics 12, 2070-80
23608596   Curated Info

5

Humphrey SJ, et al. (2013) Dynamic Adipocyte Phosphoproteome Reveals that Akt Directly Regulates mTORC2. Cell Metab 17, 1009-20
23684622   Curated Info

6

Grimsrud PA, et al. (2012) A quantitative map of the liver mitochondrial phosphoproteome reveals posttranslational control of ketogenesis. Cell Metab 16, 672-83
23140645   Curated Info

7

Knight JD, et al. (2012) A novel whole-cell lysate kinase assay identifies substrates of the p38 MAPK in differentiating myoblasts. Skelet Muscle 2, 5
22394512   Curated Info

8

Yu Y, et al. (2011) Phosphoproteomic analysis identifies Grb10 as an mTORC1 substrate that negatively regulates insulin signaling. Science 332, 1322-6
21659605   Curated Info

9

Huttlin EL, et al. (2010) A tissue-specific atlas of mouse protein phosphorylation and expression. Cell 143, 1174-89
21183079   Curated Info

10

Han MY, Kosako H, Watanabe T, Hattori S (2007) Extracellular Signal-Regulated Kinase/Mitogen-Activated Protein Kinase Regulates Actin Organization and Cell Motility by Phosphorylating the Actin Cross-Linking Protein EPLIN. Mol Cell Biol 27, 8190-8204
17875928   Curated Info