Ser102
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Home > Phosphorylation Site Page: > Ser102  -  PDE5A (human)

Site Information
GTPTRKIsAsEFDRP   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448178

In vivo Characterization
Methods used to characterize site in vivo:
mass spectrometry ( 1 , 2 , 5 ) , mutation of modification site ( 3 ) , phospho-antibody ( 8 ) , western blotting ( 8 )
Disease tissue studied:
breast cancer ( 2 ) , HER2 positive breast cancer ( 1 ) , luminal A breast cancer ( 1 ) , luminal B breast cancer ( 1 ) , breast cancer, surrounding tissue ( 1 ) , breast cancer, triple negative ( 1 , 2 ) , neuroblastoma ( 3 ) , ovarian cancer ( 2 )
Relevant cell line - cell type - tissue:
A498 (renal) ( 5 ) , breast ( 1 , 2 ) , ovary ( 2 ) , SKNAS (neural crest) ( 3 ) , uterus ( 8 )

Upstream Regulation
Kinases, in vitro:
PKACA (cow) ( 9 ) , PKACA (human) ( 6 , 7 , 8 ) , PKG1 (cow) ( 9 ) , PKG1 (human) ( 8 )
Phosphatases, in vitro:
PPP1CA (human) ( 8 )
Treatments:
calyculin_A ( 8 ) , cAMP_analog ( 8 ) , cGMP_analog ( 8 ) , okadaic_acid ( 8 )

Downstream Regulation
Effects of modification on PDE5A:
enzymatic activity, induced ( 6 ) , enzymatic activity, inhibited ( 3 ) , molecular association, regulation ( 6 ) , protein conformation ( 3 )
Induce interaction with:
Other ( 6 )

References 

1

Mertins P, et al. (2016) Proteogenomics connects somatic mutations to signalling in breast cancer. Nature 534, 55-62
27251275   Curated Info

2

Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704
24719451   Curated Info

3

Carøe Nordgaard J, Kruse LS, Gammeltoft S, Kruuse CR (2014) Role of Ser102 and Ser104 as Regulators of cGMP Hydrolysis by PDE5A. PLoS One 9, e107627
25247292   Curated Info

4

Shiromizu T, et al. (2013) Identification of missing proteins in the neXtProt database and unregistered phosphopeptides in the PhosphoSitePlus database as part of the Chromosome-centric Human Proteome Project. J Proteome Res 12, 2414-21
23312004   Curated Info

5

Schreiber TB, et al. (2010) An integrated phosphoproteomics work flow reveals extensive network regulation in early lysophosphatidic acid signaling. Mol Cell Proteomics 9, 1047-62
20071362   Curated Info

6

Bruder S, Schultz A, Schultz JE (2006) Characterization of the tandem GAF domain of human phosphodiesterase 5 using a cyanobacterial adenylyl cyclase as a reporter enzyme. J Biol Chem 281, 19969-76
16690614   Curated Info

7

Francis SH, et al. (2002) Phosphorylation of isolated human phosphodiesterase-5 regulatory domain induces an apparent conformational change and increases cGMP binding affinity. J Biol Chem 277, 47581-7
12359732   Curated Info

8

Rybalkin SD, et al. (2002) Regulation of cGMP-specific phosphodiesterase (PDE5) phosphorylation in smooth muscle cells. J Biol Chem 277, 3310-7
11723116   Curated Info

9

Thomas MK, Francis SH, Corbin JD (1990) Substrate- and kinase-directed regulation of phosphorylation of a cGMP-binding phosphodiesterase by cGMP. J Biol Chem 265, 14971-8
2168396   Curated Info