Ser46
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Home > Phosphorylation Site Page: > Ser46  -  SIRT1 (mouse)

Site Information
DGPGLGRsPGEPsAA   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 452899

In vivo Characterization
Methods used to characterize site in vivo:
mass spectrometry ( 2 , 5 , 6 , 7 , 9 , 10 ) , mutation of modification site ( 8 ) , phospho-antibody ( 3 , 8 ) , western blotting ( 3 , 8 )
Disease tissue studied:
anthrax infection ( 9 )
Relevant cell line - cell type - tissue:
'3T3-L1, differentiated' (adipocyte) ( 5 , 6 ) , BaF3 ('B lymphocyte, precursor') [JAK3 (human), transfection] ( 2 ) , kidney ( 10 ) , lung ( 10 ) , macrophage-peritoneum [MPRIP (mouse), homozygous knockout] ( 7 ) , MEF (fibroblast) [SIRT1 (mouse), homozygous knockout] ( 8 ) , RAW 264.7 (macrophage) ( 3 ) , spleen ( 9 ) , testis ( 10 )

Upstream Regulation
Regulatory protein:
PPAR-gamma (mouse) ( 3 )
Putative in vivo kinases:
JNK1 (mouse) ( 8 )
Treatments:
GW_9662 ( 3 ) , LPS ( 3 ) , rosiglitazone ( 3 )

Downstream Regulation
Effects of modification on SIRT1:
protein degradation ( 8 ) , ubiquitination ( 8 )

Disease / Diagnostics Relevance
Relevant diseases:
obesity, hyperphagic ( 8 )

References 

1

Yao JY, et al. (2021) Novel CDK9 inhibitor oroxylin A promotes wild-type P53 stability and prevents hepatocellular carcinoma progression by disrupting both MDM2 and SIRT1 signaling. Acta Pharmacol Sin
34188177   Curated Info

2

Degryse S, et al. (2017) Mutant JAK3 phosphoproteomic profiling predicts synergism between JAK3 inhibitors and MEK/BCL2 inhibitors for the treatment of T-cell acute lymphoblastic leukemia. Leukemia
28852199   Curated Info

3

Hwang JS, et al. (2016) Upregulation of MKP-7 in response to rosiglitazone treatment ameliorates lipopolysaccharide-induced destabilization of SIRT1 by inactivating JNK. Pharmacol Res 114, 47-55
27771463   Curated Info

4

Igarashi M, Guarente L (2016) mTORC1 and SIRT1 Cooperate to Foster Expansion of Gut Adult Stem Cells during Calorie Restriction. Cell 166, 436-50
27345368   Curated Info

5

Parker BL, et al. (2015) Targeted phosphoproteomics of insulin signaling using data-independent acquisition mass spectrometry. Sci Signal 8, rs6
26060331   Curated Info

6

Humphrey SJ, et al. (2013) Dynamic Adipocyte Phosphoproteome Reveals that Akt Directly Regulates mTORC2. Cell Metab 17, 1009-20
23684622   Curated Info

7

Wu X, et al. (2012) Investigation of receptor interacting protein (RIP3)-dependent protein phosphorylation by quantitative phosphoproteomics. Mol Cell Proteomics 11, 1640-51
22942356   Curated Info

8

Gao Z, et al. (2011) Sirtuin 1 (SIRT1) protein degradation in response to persistent c-Jun N-terminal kinase 1 (JNK1) activation contributes to hepatic steatosis in obesity. J Biol Chem 286, 22227-34
21540183   Curated Info

9

Manes NP, et al. (2011) Discovery of mouse spleen signaling responses to anthrax using label-free quantitative phosphoproteomics via mass spectrometry. Mol Cell Proteomics 10, M110.000927
21189417   Curated Info

10

Huttlin EL, et al. (2010) A tissue-specific atlas of mouse protein phosphorylation and expression. Cell 143, 1174-89
21183079   Curated Info