Ser204
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Home > Phosphorylation Site Page: > Ser204  -  PAK1 (human)

Site Information
tKsVyTRsVIEPLPV   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448173

In vivo Characterization
Methods used to characterize site in vivo:
immunoassay ( 2 ) , immunoprecipitation ( 2 , 3 , 10 ) , mass spectrometry ( 2 , 4 , 5 , 7 , 8 , 14 , 15 , 17 , 18 ) , mutation of modification site ( 2 , 10 ) , phospho-antibody ( 3 , 6 , 10 , 13 , 16 , 19 ) , western blotting ( 1 , 2 , 3 , 6 , 10 , 13 , 16 , 19 )
Disease tissue studied:
bone cancer ( 6 ) , breast cancer ( 2 , 3 , 7 , 13 ) , breast adenocarcinoma ( 2 ) , breast ductal carcinoma ( 7 ) , HER2 positive breast cancer ( 4 ) , luminal A breast cancer ( 4 ) , luminal B breast cancer ( 4 ) , breast cancer, triple negative ( 2 , 3 , 4 , 7 ) , leukemia ( 2 , 10 ) , T cell leukemia ( 2 , 10 ) , lung cancer ( 1 ) , non-small cell lung cancer ( 1 ) , non-small cell lung adenocarcinoma ( 1 ) , non-small cell squamous cell lung carcinoma ( 1 ) , prostate cancer ( 3 ) , melanoma skin cancer ( 5 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Regulatory protein:
Akt1 (human) ( 20 ) , CDC42 iso1 (human) ( 16 ) , RAC1 (human) ( 6 , 20 )
Putative in vivo kinases:
MLK3 (human) ( 2 ) , PAK1 (human) ( 10 )
Kinases, in vitro:
MLK3 (human) ( 2 )
Treatments:
anti-CD3/CD28 ( 2 ) , Auranofin ( 1 ) , bafetinib ( 3 ) , CEP-11004 ( 2 ) , CGP53353 ( 6 ) , CXCL12 ( 19 ) , doxycycline ( 2 ) , fibronectin ( 6 ) , Go_6976 ( 6 ) , IPA-3 ( 1 ) , ischemia ( 7 ) , LPS ( 20 ) , osimertinib ( 1 ) , OTSSP167 ( 1 ) , TNF ( 2 ) , wortmannin ( 20 )

Downstream Regulation
Effects of modification on PAK1:
enzymatic activity, induced ( 2 , 16 ) , intracellular localization ( 16 ) , molecular association, regulation ( 2 ) , phosphorylation ( 2 ) , protein conformation ( 16 )
Effects of modification on biological processes:
carcinogenesis, induced ( 1 , 2 ) , cell adhesion, altered ( 16 ) , cell growth, induced ( 1 , 2 ) , cell motility, altered ( 16 ) , cell motility, induced ( 2 ) , signaling pathway regulation ( 2 )
Inhibit interaction with:
PXN (human) ( 2 )

Disease / Diagnostics Relevance
Relevant diseases:
breast cancer ( 2 ) , non-small cell lung cancer ( 1 )

References 

1

Ito M, et al. (2019) Targeting PKCι-PAK1 signaling pathways in EGFR and KRAS mutant adenocarcinoma and lung squamous cell carcinoma. Cell Commun Signal 17, 137
31660987   Curated Info

2

Das S, et al. (2019) Mixed lineage kinase 3 promotes breast tumorigenesis via phosphorylation and activation of p21-activated kinase 1. Oncogene
30664689   Curated Info

3

Thaper D, et al. (2017) Targeting Lyn regulates Snail family shuttling and inhibits metastasis. Oncogene 36, 3964-3975
28288135   Curated Info

4

Mertins P, et al. (2016) Proteogenomics connects somatic mutations to signalling in breast cancer. Nature 534, 55-62
27251275   Curated Info

5

Stuart SA, et al. (2015) A Phosphoproteomic Comparison of B-RAFV600E and MKK1/2 Inhibitors in Melanoma Cells. Mol Cell Proteomics 14, 1599-615
25850435   Curated Info

6

Pasapera AM, et al. (2015) Rac1-Dependent Phosphorylation and Focal Adhesion Recruitment of Myosin IIA Regulates Migration and Mechanosensing. Curr Biol 25, 175-86
25544611   Curated Info

7

Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704
24719451   Curated Info

8

Mertins P, et al. (2013) Integrated proteomic analysis of post-translational modifications by serial enrichment. Nat Methods 10, 634-7
23749302   Curated Info

9

Shiromizu T, et al. (2013) Identification of missing proteins in the neXtProt database and unregistered phosphopeptides in the PhosphoSitePlus database as part of the Chromosome-centric Human Proteome Project. J Proteome Res 12, 2414-21
23312004   Curated Info

10

Rouquette-Jazdanian AK, et al. (2012) LAT-Independent Erk Activation via Bam32-PLC-γ1-Pak1 Complexes: GTPase-Independent Pak1 Activation. Mol Cell 48, 298-312
22981863   Curated Info

11

Klammer M, et al. (2012) Phosphosignature predicts dasatinib response in non-small cell lung cancer. Mol Cell Proteomics 11, 651-68
22617229   Curated Info

12

Beli P, et al. (2012) Proteomic Investigations Reveal a Role for RNA Processing Factor THRAP3 in the DNA Damage Response. Mol Cell 46, 212-25
22424773   Curated Info

13

Jung J, et al. (2011) Translationally controlled tumor protein induces human breast epithelial cell transformation through the activation of Src. Oncogene 30, 2264-74
21278788   Curated Info

14

Rigbolt KT, et al. (2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation. Sci Signal 4, rs3
21406692   Curated Info

15

Højlund K, et al. (2009) In vivo phosphoproteome of human skeletal muscle revealed by phosphopeptide enrichment and HPLC-ESI-MS/MS. J Proteome Res 8, 4954-65
19764811   Curated Info

16

Parrini MC, Camonis J, Matsuda M, de Gunzburg J (2009) Dissecting activation of the PAK1 kinase at protrusions in living cells. J Biol Chem 284, 24133-43
19574218   Curated Info

17

Brill LM, et al. (2009) Phosphoproteomic analysis of human embryonic stem cells. Cell Stem Cell 5, 204-13
19664994   Curated Info

18

Mayya V, et al. (2009) Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions. Sci Signal 2, ra46
19690332   Curated Info

19

Rousseau S, et al. (2006) CXCL12 and C5a trigger cell migration via a PAK1/2-p38alpha MAPK-MAPKAP-K2-HSP27 pathway. Cell Signal 18, 1897-905
16574378   Curated Info

20

Basak C, et al. (2005) NF-kappaB- and C/EBPbeta-driven interleukin-1beta gene expression and PAK1-mediated caspase-1 activation play essential roles in interleukin-1beta release from Helicobacter pylori lipopolysaccharide-stimulated macrophages. J Biol Chem 280, 4279-88
15561713   Curated Info