Ser73
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Home > Phosphorylation Site Page: > Ser73  -  BIM (mouse)

Site Information
PGPFATRsPLFIFVR   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 3306339

In vivo Characterization
Methods used to characterize site in vivo:
2D analysis ( 7 ) , flow cytometry ( 1 ) , immunoprecipitation ( 1 ) , mass spectrometry ( 2 , 3 , 4 , 5 , 6 ) , mutation of modification site ( 1 , 7 ) , phospho-antibody ( 7 ) , western blotting ( 1 , 7 )
Relevant cell line - cell type - tissue:
'3T3-L1, differentiated' (adipocyte) ( 2 ) , 293 (epithelial) ( 7 ) , 32Dcl3 (myeloid) [FLT3 (mouse), transfection, chimera with human FLT3-ITD mutant (corresponding to wild type P36888 ( 6 ) , epithelial-mammary gland ( 1 ) , ES-J1 (stem) ( 4 , 5 ) , HEK293T (epithelial) ( 1 ) , kidney ( 1 , 3 ) , lung ( 3 ) , MEF (fibroblast) ( 7 ) , spleen ( 3 )

Upstream Regulation
Treatments:
insulin ( 2 ) , serum ( 7 )

Downstream Regulation
Effects of modification on BIM:
protein degradation ( 7 )
Effects of modification on biological processes:
apoptosis, inhibited ( 7 )

References 

1

Girnius N, Davis RJ (2017) JNK Promotes Epithelial Cell Anoikis by Transcriptional and Post-translational Regulation of BH3-Only Proteins. Cell Rep 21, 1910-1921
29141222   Curated Info

2

Humphrey SJ, et al. (2013) Dynamic Adipocyte Phosphoproteome Reveals that Akt Directly Regulates mTORC2. Cell Metab 17, 1009-20
23684622   Curated Info

3

Huttlin EL, et al. (2010) A tissue-specific atlas of mouse protein phosphorylation and expression. Cell 143, 1174-89
21183079   Curated Info

4

Zhou J (2010) CST Curation Set: 9670; Year: 2010; Biosample/Treatment: cell line, ES J1/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-(Ser/Thr) PKD Substrate Antibody Cat#: 4381, PTMScan(R) Phospho-PKD Substrate Motif (LXRXXpS/pT) Immunoaffinity Beads Cat#: 1986
Curated Info

5

Zhou J (2010) CST Curation Set: 9676; Year: 2010; Biosample/Treatment: cell line, ES J1/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RRXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-PKA Substrate (RRXS/T) (100G7) Rabbit mAb Cat#: 9624, PTMScan(R) Phospho-PKA Substrate Motif (K/RK/RXS*/T*) Immunoaffinity Beads Cat#: 1984
Curated Info

6

Choudhary C, et al. (2009) Mislocalized activation of oncogenic RTKs switches downstream signaling outcomes. Mol Cell 36, 326-39
19854140   Curated Info

7

Hübner A, Barrett T, Flavell RA, Davis RJ (2008) Multisite phosphorylation regulates Bim stability and apoptotic activity. Mol Cell 30, 415-25
18498746   Curated Info