Thr370
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Home > Phosphorylation Site Page: > Thr370  -  MOR-1 (rat)

Site Information
sTRVRQNtREHPsTA   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 447785

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 6 ) , mass spectrometry ( 2 ) , microscopy-colocalization with upstream kinase ( 1 ) , mutation of modification site ( 3 , 4 , 5 , 6 ) , phospho-antibody ( 1 , 4 ) , western blotting ( 1 , 4 )
Disease tissue studied:
neuroblastoma ( 5 ) , pituitary cancer ( 4 )
Relevant cell line - cell type - tissue:
'brain, striatum' ( 4 ) , 293 (epithelial) ( 2 , 3 , 4 , 6 ) , AtT20 (pituitary cell) ( 4 ) , MEF (fibroblast) ( 3 ) , Neuro-2a (neuron) ( 5 ) , neuron-dorsal root ganglia ( 1 )

Upstream Regulation
Regulatory protein:
PKCA (rat) ( 1 ) , RAGE (rat) ( 1 )
Putative in vivo kinases:
PKCA (rat) ( 1 )
Kinases, in vitro:
CAMK2A (human) ( 2 )
Treatments:
DAMGO ( 4 , 6 ) , etorphine ( 5 ) , naloxone ( 4 )

Downstream Regulation
Effects of modification on MOR-1:
molecular association, regulation ( 2 ) , phosphorylation ( 3 ) , receptor desensitization, altered ( 3 , 5 ) , receptor internalization, altered ( 5 , 6 )
Induce interaction with:
ARR3 (human) ( 2 ) , ARRB2 (human) ( 2 )

Disease / Diagnostics Relevance
Relevant diseases:
diabetes mellitus ( 1 )

References 

1

Mousa SA, et al. (2016) Protein kinase C-mediated mu-opioid receptor phosphorylation and desensitization in rats, and its prevention during early diabetes. Pain 157, 910-21
26713421   Curated Info

2

Chen YJ, et al. (2013) Identification of phosphorylation sites in the COOH-terminal tail of the μ-opioid receptor. J Neurochem 124, 189-99
23106126   Curated Info

3

Chu J, Zheng H, Loh HH, Law PY (2008) Morphine-induced mu-opioid receptor rapid desensitization is independent of receptor phosphorylation and beta-arrestins. Cell Signal 20, 1616-24
18558479   Curated Info

4

Petraschka M, et al. (2007) The absence of endogenous beta-endorphin selectively blocks phosphorylation and desensitization of mu opioid receptors following partial sciatic nerve ligation. Neuroscience 146, 1795-807
17467916   Curated Info

5

Qiu Y, Law PY, Loh HH (2003) Mu-opioid receptor desensitization: role of receptor phosphorylation, internalization, and representation. J Biol Chem 278, 36733-9
12860981   Curated Info

6

El Kouhen R, et al. (2001) Phosphorylation of Ser363, Thr370, and Ser375 residues within the carboxyl tail differentially regulates mu-opioid receptor internalization. J Biol Chem 276, 12774-80
11278523   Curated Info