Ser210
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Home > Phosphorylation Site Page: > Ser210  -  PPM1H (mouse)

Site Information
RTLTRAAsLrGGVGA   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 3215799

In vivo Characterization
Methods used to characterize site in vivo:
[32P] ATP in vitro ( 1 ) , electrophoretic mobility shift ( 1 ) , mass spectrometry ( 3 , 5 , 6 , 7 , 8 , 9 , 10 , 11 ) , mutation of modification site ( 1 ) , phospho-antibody ( 1 ) , western blotting ( 1 )
Disease tissue studied:
neuroblastoma ( 1 , 7 )
Relevant cell line - cell type - tissue:
'fat, brown' ( 8 ) , brain ( 5 , 6 , 8 , 10 , 11 ) , HEK293T (epithelial) ( 1 ) , kidney ( 8 ) , liver ( 8 ) , macrophage-bone marrow ( 9 ) , macrophage-bone marrow [DUSP1 (mouse), homozygous knockout] ( 9 ) , N1E-115 (neuron) ( 7 ) , Neuro-2a (neuron) ( 1 ) , pancreas ( 8 ) , RAW 264.7 (macrophage) ( 3 ) , spleen ( 8 )

Upstream Regulation
Regulatory protein:
PPM1H (mouse) ( 1 )
Putative in vivo kinases:
CAMK1A (mouse) ( 1 )
Kinases, in vitro:
CAMK1A (mouse) ( 1 )
Treatments:
ionomycin ( 1 ) , STO-609 ( 1 )

Downstream Regulation
Effects of modification on PPM1H:
enzymatic activity, induced ( 1 ) , phosphorylation ( 1 )

References 

1

Osawa J, et al. (2020) Dual phosphorylation of protein phosphatase PPM1H promotes dephosphorylation of Smad1 in cellulo. Biochem Biophys Res Commun 530, 513-519
32600616   Curated Info

2

Sacco F, et al. (2016) Glucose-regulated and drug-perturbed phosphoproteome reveals molecular mechanisms controlling insulin secretion. Nat Commun 7, 13250
27841257   Curated Info

3

Pinto SM, et al. (2015) Quantitative phosphoproteomic analysis of IL-33-mediated signaling. Proteomics 15, 532-44
25367039   Curated Info

4

Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704
24719451   Curated Info

5

Trinidad JC, et al. (2012) Global identification and characterization of both O-GlcNAcylation and phosphorylation at the murine synapse. Mol Cell Proteomics 11, 215-29
22645316   Curated Info

6

Goswami T, et al. (2012) Comparative phosphoproteomic analysis of neonatal and adult murine brain. Proteomics 12, 2185-9
22807455   Curated Info

7

Wang Y, et al. (2011) Spatial phosphoprotein profiling reveals a compartmentalized extracellular signal-regulated kinase switch governing neurite growth and retraction. J Biol Chem 286, 18190-201
21454597   Curated Info

8

Huttlin EL, et al. (2010) A tissue-specific atlas of mouse protein phosphorylation and expression. Cell 143, 1174-89
21183079   Curated Info

9

Weintz G, et al. (2010) The phosphoproteome of toll-like receptor-activated macrophages. Mol Syst Biol 6, 371
20531401   Curated Info

10

Possemato A (2008) CST Curation Set: 3819; Year: 2008; Biosample/Treatment: tissue, brain/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-(Ser/Thr) PKD Substrate Antibody Cat#: 4381, PTMScan(R) Phospho-PKD Substrate Motif (LXRXXpS/pT) Immunoaffinity Beads Cat#: 1986
Curated Info

11

Possemato A (2008) CST Curation Set: 3843; Year: 2008; Biosample/Treatment: tissue, brain/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: (K/R)XpSX(K/R) Antibodies Used to Purify Peptides prior to LCMS: Phospho-(Ser) PKC Substrate Antibody Cat#: 2261, PTMScan(R) Phospho-PKC Substrate Motif (K/RXpSXK/R) Immunoaffinity Beads Cat#: 1985
Curated Info

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