Ser776

Site Information
PDLEPSDsEAEGEGS SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 3208257

In vivo Characterization
Methods used to characterize site in vivo:	mass spectrometry ( 1 , 2 , 3 , 4 , 5 , 6 )
Disease tissue studied:	leukemia ( 4 ) , acute myelogenous leukemia ( 4 )
Relevant cell line - cell type - tissue:	'3T3-L1, differentiated' (adipocyte) ( 2 ) , blood ( 4 ) , kidney ( 6 ) , lung ( 6 ) , MEF (fibroblast) [Raptor (mouse), knockdown] ( 3 ) , MEF (fibroblast) [RICTOR (mouse), knockdown] ( 3 ) , MEF (fibroblast) [TSC2 (mouse), homozygous knockout] ( 5 ) , MEF (fibroblast) ( 3 , 5 ) , RAW 264.7 (macrophage) ( 1 ) , spleen ( 6 ) , testis ( 6 )

Upstream Regulation
Treatments:	insulin ( 2 ) , LY294002 ( 2 )

References
1	Pinto SM, et al. (2015) Quantitative phosphoproteomic analysis of IL-33-mediated signaling. Proteomics 15, 532-44 25367039 Curated Info
2	Humphrey SJ, et al. (2013) Dynamic Adipocyte Phosphoproteome Reveals that Akt Directly Regulates mTORC2. Cell Metab 17, 1009-20 23684622 Curated Info
3	Robitaille AM, et al. (2013) Quantitative phosphoproteomics reveal mTORC1 activates de novo pyrimidine synthesis. Science 339, 1320-3 23429704 Curated Info
4	Trost M, et al. (2012) Posttranslational regulation of self-renewal capacity: insights from proteome and phosphoproteome analyses of stem cell leukemia. Blood 120, e17-27 22802335 Curated Info
5	Yu Y, et al. (2011) Phosphoproteomic analysis identifies Grb10 as an mTORC1 substrate that negatively regulates insulin signaling. Science 332, 1322-6 21659605 Curated Info
6	Huttlin EL, et al. (2010) A tissue-specific atlas of mouse protein phosphorylation and expression. Cell 143, 1174-89 21183079 Curated Info