Ser352

Site Information
TSRtPRDsPGIPPsA SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 447989

In vivo Characterization
Methods used to characterize site in vivo:	flow cytometry ( 17 ) , immunoprecipitation ( 4 ) , mass spectrometry ( 2 , 3 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 , 14 , 16 , 18 ) , mutation of modification site ( 4 ) , phospho-antibody ( 1 , 4 , 13 , 15 , 17 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 ) , western blotting ( 1 , 4 , 13 , 15 , 17 , 19 , 20 , 22 , 24 , 26 )
Disease tissue studied:	breast cancer ( 20 ) , neuroblastoma ( 12 )
Relevant cell line - cell type - tissue:	'3T3-L1, differentiated' (adipocyte) ( 5 , 6 , 10 ) , 'brain, cerebral cortex' ( 4 , 23 ) , 'brain, striatum' ( 27 ) , 'neuron, hippocampal'-brain [neurogranin (mouse)] ( 26 ) , 32Dcl3 (myeloid) [FLT3 (mouse), transfection, chimera with human FLT3-ITD mutant (corresponding to wild type P36888 ( 18 ) , 32Dcl3 (myeloid) ( 18 ) , 3T3-L1 (fibroblast) ( 1 ) , BaF3 ('B lymphocyte, precursor') [JAK3 (human), transfection] ( 2 ) , brain ( 14 ) , colon ( 15 ) , HC11 (epithelial) ( 19 ) , heart ( 21 ) , hepatocyte-liver ( 24 ) , HL-1 (myocyte) [Akt1 (mouse), knockdown, stable lentiviral expression of Akt1 shRNA] ( 8 ) , HL-1 (myocyte) [Akt2 (mouse), knockdown, stable lentiviral expression of Akt2 shRNA] ( 8 ) , HL-1 (myocyte) ( 8 ) , JB (epithelial) ( 22 ) , kidney ( 14 ) , liver ( 3 ) , lung ( 14 ) , macrophage-bone marrow ( 16 ) , macrophage-bone marrow [DUSP1 (mouse), homozygous knockout] ( 16 ) , macrophage-peritoneum ( 11 ) , macrophage-peritoneum [MPRIP (mouse), homozygous knockout] ( 11 ) , MEF (fibroblast) ( 24 , 28 ) , Mel-Ab (melanocyte) ( 13 , 25 ) , N1E-115 (neuron) ( 12 ) , NMuMG (epithelial) ( 19 ) , RAW 264.7 (macrophage) ( 7 ) , spleen ( 14 ) , stromal ( 9 ) , T lymphocyte-lymph node ( 17 ) , T47D (breast cell) ( 20 )

Upstream Regulation
Regulatory protein:	FMR1 (mouse) ( 4 ) , neurogranin (mouse) ( 26 )
Treatments:	antibody ( 17 ) , bacterial infection ( 15 ) , colforsin ( 26 ) , EGCG ( 22 ) , EGF ( 19 , 24 ) , insulin ( 10 , 24 ) , ischemia/reperfusion ( 21 ) , LPA ( 25 ) , LPS ( 16 ) , LY294002 ( 17 ) , NP68 ( 17 ) , PD184352 ( 24 ) , phorbol_ester ( 26 ) , PP2 ( 17 ) , PRL ( 19 ) , rapamycin ( 17 , 24 ) , ravoxertinib ( 1 ) , SL327 ( 4 ) , sphingosine_1-phosphate ( 13 ) , TGF-beta ( 27 ) , Theaflavins ( 22 ) , TTX ( 23 ) , U0126 ( 17 ) , UV ( 22 , 28 ) , visual experience ( 23 )

Upstream Regulation

Regulatory protein:

FMR1 (mouse) ( 4 ) , neurogranin (mouse) ( 26 )

Treatments:

antibody ( 17 ) , bacterial infection ( 15 ) , colforsin ( 26 ) , EGCG ( 22 ) , EGF ( 19 , 24 ) , insulin ( 10 , 24 ) , ischemia/reperfusion ( 21 ) , LPA ( 25 ) , LPS ( 16 ) , LY294002 ( 17 ) , NP68 ( 17 ) , PD184352 ( 24 ) , phorbol_ester ( 26 ) , PP2 ( 17 ) , PRL ( 19 ) , rapamycin ( 17 , 24 ) , ravoxertinib ( 1 ) , SL327 ( 4 ) , sphingosine_1-phosphate ( 13 ) , TGF-beta ( 27 ) , Theaflavins ( 22 ) , TTX ( 23 ) , U0126 ( 17 ) , UV ( 22 , 28 ) , visual experience ( 23 )

Downstream Regulation
Effects of modification on p90RSK:	enzymatic activity, induced ( 25 )

References
1	Duan X, et al. (2022) Trafficking regulator of GLUT4-1 (TRARG1) is a GSK3 substrate. Biochem J 35594055 Curated Info
2	Degryse S, et al. (2017) Mutant JAK3 phosphoproteomic profiling predicts synergism between JAK3 inhibitors and MEK/BCL2 inhibitors for the treatment of T-cell acute lymphoblastic leukemia. Leukemia 32 28852199 Curated Info
3	Robles MS, Humphrey SJ, Mann M (2017) Phosphorylation Is a Central Mechanism for Circadian Control of Metabolism and Physiology. Cell Metab 25, 118-127 27818261 Curated Info
4	Sawicka K, et al. (2016) Elevated ERK/p90 ribosomal S6 kinase activity underlies audiogenic seizure susceptibility in fragile X mice. Proc Natl Acad Sci U S A 113, E6290-E6297 27663742 Curated Info
5	Minard AY, et al. (2016) mTORC1 Is a Major Regulatory Node in the FGF21 Signaling Network in Adipocytes. Cell Rep 17, 29-36 27681418 Curated Info
6	Parker BL, et al. (2015) Targeted phosphoproteomics of insulin signaling using data-independent acquisition mass spectrometry. Sci Signal 8, rs6 26060331 Curated Info
7	Pinto SM, et al. (2015) Quantitative phosphoproteomic analysis of IL-33-mediated signaling. Proteomics 15, 532-44 25367039 Curated Info
8	Reinartz M, Raupach A, Kaisers W, Gödecke A (2014) AKT1 and AKT2 induce distinct phosphorylation patterns in HL-1 cardiac myocytes. J Proteome Res 13, 4232-45 25162660 Curated Info
9	Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704 24719451 Curated Info
10	Humphrey SJ, et al. (2013) Dynamic Adipocyte Phosphoproteome Reveals that Akt Directly Regulates mTORC2. Cell Metab 17, 1009-20 23684622 Curated Info
11	Wu X, et al. (2012) Investigation of receptor interacting protein (RIP3)-dependent protein phosphorylation by quantitative phosphoproteomics. Mol Cell Proteomics 11, 1640-51 22942356 Curated Info
12	Wang Y, et al. (2011) Spatial phosphoprotein profiling reveals a compartmentalized extracellular signal-regulated kinase switch governing neurite growth and retraction. J Biol Chem 286, 18190-201 21454597 Curated Info
13	Kim DS, et al. (2011) Sphingosine-1-phosphate decreases melanin synthesis via microphthalmia-associated transcription factor phosphorylation through the S1P3 receptor subtype. J Pharm Pharmacol 63, 409-16 21749389 Curated Info
14	Huttlin EL, et al. (2010) A tissue-specific atlas of mouse protein phosphorylation and expression. Cell 143, 1174-89 21183079 Curated Info
15	Chandrakesan P, et al. (2010) Novel changes in NF-{kappa}B activity during progression and regression phases of hyperplasia: role of MEK, ERK, and p38. J Biol Chem 285, 33485-98 20710027 Curated Info
16	Weintz G, et al. (2010) The phosphoproteome of toll-like receptor-activated macrophages. Mol Syst Biol 6, 371 20531401 Curated Info
17	Salmond RJ, Emery J, Okkenhaug K, Zamoyska R (2009) MAPK, phosphatidylinositol 3-kinase, and mammalian target of rapamycin pathways converge at the level of ribosomal protein S6 phosphorylation to control metabolic signaling in CD8 T cells. J Immunol 183, 7388-97 19917692 Curated Info
18	Choudhary C, et al. (2009) Mislocalized activation of oncogenic RTKs switches downstream signaling outcomes. Mol Cell 36, 326-39 19854140 Curated Info
19	Haines E, et al. (2009) Tyrosine phosphorylation of Grb2: role in prolactin/epidermal growth factor cross talk in mammary epithelial cell growth and differentiation. Mol Cell Biol 29, 2505-20 19273609 Curated Info
20	Vicent GP, et al. (2006) Induction of progesterone target genes requires activation of Erk and Msk kinases and phosphorylation of histone H3. Mol Cell 24, 367-81 17081988 Curated Info
21	Maekawa N, et al. (2006) Inhibiting p90 ribosomal S6 kinase prevents (Na+)-H+ exchanger-mediated cardiac ischemia-reperfusion injury. Circulation 113, 2516-23 16717153 Curated Info
22	Zykova TA, et al. (2005) The signal transduction networks required for phosphorylation of STAT1 at Ser727 in mouse epidermal JB6 cells in the UVB response and inhibitory mechanisms of tea polyphenols. Carcinogenesis 26, 331-42 15550455 Curated Info
23	Suzuki S, al-Noori S, Butt SA, Pham TA (2004) Regulation of the CREB signaling cascade in the visual cortex by visual experience and neuronal activity. J Comp Neurol 479, 70-83 15389611 Curated Info
24	Pende M, et al. (2004) S6K1(-/-)/S6K2(-/-) mice exhibit perinatal lethality and rapamycin-sensitive 5'-terminal oligopyrimidine mRNA translation and reveal a mitogen-activated protein kinase-dependent S6 kinase pathway. Mol Cell Biol 24, 3112-24 15060135 Curated Info
25	Kim DS, et al. (2004) Effects of lysophosphatidic acid on melanogenesis. Chem Phys Lipids 127, 199-206 14726002 Curated Info
26	Wu J, Li J, Huang KP, Huang FL (2002) Attenuation of protein kinase C and cAMP-dependent protein kinase signal transduction in the neurogranin knockout mouse. J Biol Chem 277, 19498-505 11912190 Curated Info
27	Zhu Y, et al. (2002) Transforming growth factor-beta 1 increases bad phosphorylation and protects neurons against damage. J Neurosci 22, 3898-909 12019309 Curated Info
28	Zhang Y, et al. (2001) UVA induces Ser381 phosphorylation of p90RSK/MAPKAP-K1 via ERK and JNK pathways. J Biol Chem 276, 14572-80 11278279 Curated Info