Ser577

Site Information
PsPEPPPsPLQVPTY SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 3213141

In vivo Characterization
Methods used to characterize site in vivo:	electrophoretic mobility shift ( 2 ) , immunoprecipitation ( 2 ) , mass spectrometry ( 1 , 2 , 3 , 5 , 6 , 7 , 8 )
Disease tissue studied:	breast cancer ( 3 ) , breast ductal carcinoma ( 3 ) , HER2 positive breast cancer ( 1 ) , luminal A breast cancer ( 1 ) , luminal B breast cancer ( 1 ) , breast cancer, triple negative ( 1 ) , leukemia ( 5 , 8 ) , acute myelogenous leukemia ( 5 ) , chronic myelogenous leukemia ( 8 )
Relevant cell line - cell type - tissue:	breast ( 1 , 3 ) , HeLa (cervical) ( 2 , 6 , 7 ) , K562 (erythroid) ( 8 ) , KG-1 (myeloid) ( 5 )

Upstream Regulation
Putative in vivo kinases:	CDK1 (human) ( 2 )
Treatments:	nocodazole ( 2 ) , purvalanol ( 2 )

References
1	Mertins P, et al. (2016) Proteogenomics connects somatic mutations to signalling in breast cancer. Nature 534, 55-62 27251275 Curated Info
2	Mori Y, et al. (2015) Phosphorylation of the centrosomal protein, Cep169, by Cdk1 promotes its dissociation from centrosomes in mitosis. Biochem Biophys Res Commun 468, 642-6 26549230 Curated Info
3	Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704 24719451 Curated Info
4	Beli P, et al. (2012) Proteomic Investigations Reveal a Role for RNA Processing Factor THRAP3 in the DNA Damage Response. Mol Cell 46, 212-25 22424773 Curated Info
5	Weber C, Schreiber TB, Daub H (2012) Dual phosphoproteomics and chemical proteomics analysis of erlotinib and gefitinib interference in acute myeloid leukemia cells. J Proteomics 75, 1343-56 22115753 Curated Info
6	Kettenbach AN, et al. (2011) Quantitative phosphoproteomics identifies substrates and functional modules of aurora and polo-like kinase activities in mitotic cells. Sci Signal 4, rs5 21712546 Curated Info
7	Dephoure N, et al. (2008) A quantitative atlas of mitotic phosphorylation. Proc Natl Acad Sci U S A 105, 10762-7 18669648 Curated Info
8	Stokes M (2008) CST Curation Set: 4390; Year: 2008; Biosample/Treatment: cell line, K562/untreated; Disease: chronic myelogenous leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[STY]) Curated Info