Ser154

Site Information
LERAAAGsPRsPGPL SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 3208593

In vivo Characterization
Methods used to characterize site in vivo:	mass spectrometry ( 1 , 2 , 4 , 6 , 7 , 8 , 10 , 11 , 12 , 13 , 14 , 15 , 16 )
Disease tissue studied:	breast cancer ( 1 , 7 ) , leukemia ( 15 ) , chronic myelogenous leukemia ( 15 ) , lung cancer ( 7 ) , non-small cell lung cancer ( 7 ) , pancreatic ductal adenocarcinoma ( 2 )
Relevant cell line - cell type - tissue:	'pancreatic, ductal'-pancreas ( 2 ) , 293 (epithelial) [AT1 (human), transfection, AT1R stable transfected HEK293] ( 12 ) , 293 (epithelial) [AT1 (human), transfection] ( 11 ) , BT-20 (breast cell) ( 7 ) , Flp-In T-Rex-293 (epithelial) [PRKD1 (human), genetic knockin] ( 8 ) , Flp-In T-Rex-293 (epithelial) ( 8 ) , GM00130 (B lymphocyte) ( 13 ) , H2009 (pulmonary) ( 7 ) , H2077 (pulmonary) ( 7 ) , H2887 (pulmonary) ( 7 ) , H322M (pulmonary) ( 7 ) , HCC1359 (pulmonary) ( 7 ) , HCC4006 (pulmonary) ( 7 ) , HCC827 (pulmonary) ( 7 ) , HeLa (cervical) ( 6 , 10 , 14 ) , HMLER ('stem, breast cancer') [CXCR4 (human), knockdown] ( 1 ) , HMLER ('stem, breast cancer') ( 1 ) , HOP62 (pulmonary) ( 7 ) , Jurkat (T lymphocyte) ( 4 , 16 ) , K562 (erythroid) ( 6 , 15 ) , LCLC-103H (pulmonary) ( 7 ) , LOU-NH91 (squamous) ( 7 ) , MDA-MB-468 (breast cell) ( 7 ) , NCI-H1568 (pulmonary) ( 7 ) , NCI-H157 (pulmonary) ( 7 ) , NCI-H1666 (pulmonary) ( 7 ) , NCI-H322 (pulmonary) ( 7 ) , NCI-H460 (pulmonary) ( 7 ) , NCI-H647 (pulmonary) ( 7 )

Upstream Regulation
Treatments:	BI2536 ( 10 ) , MLN8054 ( 10 )

References
1	Yi T, et al. (2014) Quantitative phosphoproteomic analysis reveals system-wide signaling pathways downstream of SDF-1/CXCR4 in breast cancer stem cells. Proc Natl Acad Sci U S A 111, E2182-90 24782546 Curated Info
2	Britton D, et al. (2014) Quantification of pancreatic cancer proteome and phosphorylome: indicates molecular events likely contributing to cancer and activity of drug targets. PLoS One 9, e90948 24670416 Curated Info
3	Schweppe DK, Rigas JR, Gerber SA (2013) Quantitative phosphoproteomic profiling of human non-small cell lung cancer tumors. J Proteomics 91, 286-96 23911959 Curated Info
4	Mertins P, et al. (2013) Integrated proteomic analysis of post-translational modifications by serial enrichment. Nat Methods 10, 634-7 23749302 Curated Info
5	Shiromizu T, et al. (2013) Identification of missing proteins in the neXtProt database and unregistered phosphopeptides in the PhosphoSitePlus database as part of the Chromosome-centric Human Proteome Project. J Proteome Res 12, 2414-21 23312004 Curated Info
6	Zhou H, et al. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome. J Proteome Res 12, 260-71 23186163 Curated Info
7	Klammer M, et al. (2012) Phosphosignature predicts dasatinib response in non-small cell lung cancer. Mol Cell Proteomics 11, 651-68 22617229 Curated Info
8	Franz-Wachtel M, et al. (2012) Global detection of protein kinase D-dependent phosphorylation events in nocodazole-treated human cells. Mol Cell Proteomics 11, 160-70 22496350 Curated Info
9	Beli P, et al. (2012) Proteomic Investigations Reveal a Role for RNA Processing Factor THRAP3 in the DNA Damage Response. Mol Cell 46, 212-25 22424773 Curated Info
10	Kettenbach AN, et al. (2011) Quantitative phosphoproteomics identifies substrates and functional modules of aurora and polo-like kinase activities in mitotic cells. Sci Signal 4, rs5 21712546 Curated Info
11	Xiao K, et al. (2010) Global phosphorylation analysis of beta-arrestin-mediated signaling downstream of a seven transmembrane receptor (7TMR). Proc Natl Acad Sci U S A 107, 15299-304 20686112 Curated Info
12	Christensen GL, et al. (2010) Quantitative phosphoproteomics dissection of seven-transmembrane receptor signaling using full and biased agonists. Mol Cell Proteomics 9, 1540-53 20363803 Curated Info
13	Bennetzen MV, et al. (2010) Site-specific phosphorylation dynamics of the nuclear proteome during the DNA damage response. Mol Cell Proteomics 9, 1314-23 20164059 Curated Info
14	Dephoure N, et al. (2008) A quantitative atlas of mitotic phosphorylation. Proc Natl Acad Sci U S A 105, 10762-7 18669648 Curated Info
15	Stokes M (2008) CST Curation Set: 4393; Year: 2008; Biosample/Treatment: cell line, K562/untreated; Disease: chronic myelogenous leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[STY]) Curated Info
16	Stokes M (2008) CST Curation Set: 3885; Year: 2008; Biosample/Treatment: cell line, Jurkat/pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[STY]) Curated Info