Ser435

Site Information
REAELLtsAEkRAEM SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 3207627

In vivo Characterization
Methods used to characterize site in vivo:	immunoprecipitation ( 2 , 5 ) , mass spectrometry ( 1 , 3 , 4 , 5 , 6 , 7 ) , mutation of modification site ( 2 , 5 ) , phospho-antibody ( 5 ) , western blotting ( 2 )
Relevant cell line - cell type - tissue:	HeLa (cervical) ( 1 , 2 , 3 , 4 , 5 , 6 ) , HeLa S3 (cervical) ( 7 ) , HeLa_BI (cervical) ( 4 ) , HeLa_Hesp (cervical) ( 4 ) , K562 (erythroid) ( 3 )

Upstream Regulation
Putative in vivo kinases:	TTK (human) ( 5 )
Treatments:	AZ3146 ( 2 ) , BI2536 ( 2 ) , hesperadin ( 5 ) , nocodazole ( 5 ) , seliciclib ( 5 ) , SP600125 ( 2 ) , staurosporine ( 5 ) , ZM447439 ( 2 )

References
1	Sharma K, et al. (2014) Ultradeep human phosphoproteome reveals a distinct regulatory nature of Tyr and Ser/Thr-based signaling. Cell Rep 8, 1583-94 25159151 Curated Info
2	Tipton AR, et al. (2013) Monopolar Spindle 1 (MPS1) Kinase Promotes Production of Closed MAD2 (C-MAD2) Conformer and Assembly of the Mitotic Checkpoint Complex. J Biol Chem 288, 35149-58 24151075 Curated Info
3	Zhou H, et al. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome. J Proteome Res 12, 260-71 23186163 Curated Info
4	Hegemann B, et al. (2011) Systematic phosphorylation analysis of human mitotic protein complexes. Sci Signal 4, rs12 22067460 Curated Info
5	Huang H, et al. (2008) Phosphorylation sites in BubR1 that regulate kinetochore attachment, tension, and mitotic exit. J Cell Biol 183, 667-80 19015317 Curated Info
6	Dephoure N, et al. (2008) A quantitative atlas of mitotic phosphorylation. Proc Natl Acad Sci U S A 105, 10762-7 18669648 Curated Info
7	Steen JA, et al. (2008) Different phosphorylation states of the anaphase promoting complex in response to antimitotic drugs: a quantitative proteomic analysis. Proc Natl Acad Sci U S A 105, 6069-74 18420821 Curated Info