Ser546

Site Information
rGVLHTFsPsPkLQN SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 3205152

In vivo Characterization
Methods used to characterize site in vivo:	mass spectrometry ( 1 , 2 , 3 , 4 , 6 , 7 , 8 , 9 , 10 )
Disease tissue studied:	breast cancer ( 3 , 7 ) , breast ductal carcinoma ( 3 ) , HER2 positive breast cancer ( 1 ) , luminal A breast cancer ( 1 ) , luminal B breast cancer ( 1 ) , breast cancer, surrounding tissue ( 1 ) , breast cancer, triple negative ( 1 , 3 ) , cervical cancer ( 8 ) , cervical adenocarcinoma ( 8 ) , lung cancer ( 7 ) , non-small cell lung cancer ( 7 ) , ovarian cancer ( 3 )
Relevant cell line - cell type - tissue:	breast ( 1 , 3 ) , BT-20 (breast cell) ( 7 ) , BT-549 (breast cell) ( 7 ) , Calu 6 (pulmonary) ( 7 ) , HCC1359 (pulmonary) ( 7 ) , HCC1937 (breast cell) ( 7 ) , HCC2279 (pulmonary) ( 7 ) , HCC366 (pulmonary) ( 7 ) , HCC4006 (pulmonary) ( 7 ) , HCC78 (pulmonary) ( 7 ) , HeLa (cervical) ( 2 , 6 , 9 , 10 ) , HeLa S3 (cervical) ( 8 ) , Jurkat (T lymphocyte) ( 4 ) , K562 (erythroid) ( 6 ) , MCF-7 (breast cell) ( 7 ) , MDA-MB-231 (breast cell) ( 7 ) , MDA-MB-468 (breast cell) ( 7 ) , NCI-H1568 (pulmonary) ( 7 ) , NCI-H157 (pulmonary) ( 7 ) , NCI-H1648 (pulmonary) ( 7 ) , NCI-H1666 (pulmonary) ( 7 ) , NCI-H2172 (pulmonary) ( 7 ) , NCI-H520 (squamous) ( 7 ) , NCI-H647 (pulmonary) ( 7 ) , ovary ( 3 ) , PC9 (pulmonary) ( 7 )

Upstream Regulation
Treatments:	nocodazole ( 8 )

References
1	Mertins P, et al. (2016) Proteogenomics connects somatic mutations to signalling in breast cancer. Nature 534, 55-62 27251275 Curated Info
2	Sharma K, et al. (2014) Ultradeep human phosphoproteome reveals a distinct regulatory nature of Tyr and Ser/Thr-based signaling. Cell Rep 8, 1583-94 25159151 Curated Info
3	Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704 24719451 Curated Info
4	Mertins P, et al. (2013) Integrated proteomic analysis of post-translational modifications by serial enrichment. Nat Methods 10, 634-7 23749302 Curated Info
5	Shiromizu T, et al. (2013) Identification of missing proteins in the neXtProt database and unregistered phosphopeptides in the PhosphoSitePlus database as part of the Chromosome-centric Human Proteome Project. J Proteome Res 12, 2414-21 23312004 Curated Info
6	Zhou H, et al. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome. J Proteome Res 12, 260-71 23186163 Curated Info
7	Klammer M, et al. (2012) Phosphosignature predicts dasatinib response in non-small cell lung cancer. Mol Cell Proteomics 11, 651-68 22617229 Curated Info
8	Olsen JV, et al. (2010) Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis. Sci Signal 3, ra3 20068231 Curated Info
9	Dephoure N, et al. (2008) A quantitative atlas of mitotic phosphorylation. Proc Natl Acad Sci U S A 105, 10762-7 18669648 Curated Info
10	Yu LR, et al. (2007) Improved titanium dioxide enrichment of phosphopeptides from HeLa cells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra. J Proteome Res 6, 4150-62 17924679 Curated Info