Thr455

Site Information
LAMsPLPtAEtPkPL SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 31887953

In vivo Characterization
Methods used to characterize site in vivo:	immunoassay ( 1 ) , immunoprecipitation ( 2 , 3 ) , mass spectrometry ( 1 , 2 , 3 , 4 ) , mass spectrometry (in vitro) ( 1 , 2 ) , mutation of modification site ( 1 , 2 , 3 ) , phospho-antibody ( 1 ) , western blotting ( 1 , 2 , 3 )
Disease tissue studied:	colorectal cancer ( 1 ) , colorectal carcinoma ( 1 ) , lung cancer ( 1 ) , non-small cell lung cancer ( 1 ) , non-small cell lung adenocarcinoma ( 1 ) , prostate cancer ( 1 )
Relevant cell line - cell type - tissue:	A549 (pulmonary) ( 1 ) , C4-2B (prostate cell) ( 1 ) , HCT116 (intestinal) ( 1 ) , HEK293T (epithelial) ( 1 ) , HeLa (cervical) ( 2 , 3 , 4 ) , PC3 (prostate cell) ( 1 ) , RKO (intestinal) ( 1 ) , SW620 (intestinal) ( 1 )

Downstream Regulation
Effects of modification on HIF1A:	molecular association, regulation ( 1 ) , protein stabilization ( 1 ) , ubiquitination ( 1 )
Effects of modification on biological processes:	carcinogenesis, induced ( 1 ) , cell growth, induced ( 1 ) , transcription, induced ( 1 )
Inhibit interaction with:	EGLN1 (human) ( 1 )

References
1	Casillas AL, et al. (2021) Direct phosphorylation and stabilization of HIF-1α by PIM1 kinase drives angiogenesis in solid tumors. Oncogene 34211090 Curated Info
2	So L, et al. (2016) The 4E-BP-eIF4E axis promotes rapamycin-sensitive growth and proliferation in lymphocytes. Sci Signal 9, ra57 27245614 Curated Info
3	Bullen JW, et al. (2016) Protein kinase A-dependent phosphorylation stimulates the transcriptional activity of hypoxia-inducible factor 1. Sci Signal 9, ra56 27245613 Curated Info
4	Kettenbach AN, et al. (2011) Quantitative phosphoproteomics identifies substrates and functional modules of aurora and polo-like kinase activities in mitotic cells. Sci Signal 4, rs5 21712546 Curated Info