Ser122
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Home > Phosphorylation Site Page: > Ser122  -  GAPDH (human)

Site Information
GAkRVIIsAPsADAP   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 31881707

In vivo Characterization
Methods used to characterize site in vivo:
2D analysis ( 4 ) , immunoprecipitation ( 1 , 4 ) , mass spectrometry ( 1 , 2 , 3 , 4 ) , mutation of modification site ( 1 ) , phospho-antibody ( 1 ) , western blotting ( 1 )
Disease tissue studied:
lung cancer ( 2 ) , non-small cell lung adenocarcinoma ( 2 )
Relevant cell line - cell type - tissue:
293 (epithelial) ( 1 , 4 ) , HeLa (cervical) ( 3 ) , MEF (fibroblast) ( 1 ) , PC9 (pulmonary) ( 2 ) , PC9-IR (pulmonary) ( 2 )

Upstream Regulation
Kinases, in vitro:
AMPKA1 (human) ( 1 )
Treatments:
compound_C ( 1 ) , glucose_starvation ( 1 )

Downstream Regulation
Effects of modification on GAPDH:
activity, induced ( 1 ) , intracellular localization ( 1 )
Effects of modification on biological processes:
autophagy, induced ( 1 )

References 

1

Chang C, et al. (2015) AMPK-Dependent Phosphorylation of GAPDH Triggers Sirt1 Activation and Is Necessary for Autophagy upon Glucose Starvation. Mol Cell 60, 930-40
26626483   Curated Info

2

Tsai CF, et al. (2015) Large-scale determination of absolute phosphorylation stoichiometries in human cells by motif-targeting quantitative proteomics. Nat Commun 6, 6622
25814448   Curated Info

3

Kettenbach AN, et al. (2011) Quantitative phosphoproteomics identifies substrates and functional modules of aurora and polo-like kinase activities in mitotic cells. Sci Signal 4, rs5
21712546   Curated Info

4

Seo J, et al. (2008) Strategy for comprehensive identification of post-translational modifications in cellular proteins, including low abundant modifications: application to glyceraldehyde-3-phosphate dehydrogenase. J Proteome Res 7, 587-602
18183946   Curated Info

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