Ser328
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Home > Phosphorylation Site Page: > Ser328  -  NCL (human)

Site Information
PELktGIsDVFAkND   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 31867151

In vivo Characterization
Methods used to characterize site in vivo:
immunoassay ( 1 ) , immunoprecipitation ( 1 ) , mass spectrometry ( 2 , 3 , 4 ) , mutation of modification site ( 1 ) , phospho-antibody ( 1 ) , western blotting ( 1 )
Disease tissue studied:
breast cancer, triple negative ( 2 )
Relevant cell line - cell type - tissue:
breast ( 2 ) , HeLa (cervical) ( 4 ) , HUVEC (endothelial) ( 1 ) , Jurkat (T lymphocyte) ( 3 ) , MEF (fibroblast) ( 1 ) , thoracic aorta ( 1 )

Upstream Regulation
Putative in vivo kinases:
AMPKA1 (human) ( 1 )
Kinases, in vitro:
AMPKA1 (human) ( 1 )
Treatments:
acadesine ( 1 ) , metformin ( 1 ) , pulsatile shear stress ( 1 ) , siRNA ( 1 )

Downstream Regulation
Effects of modification on NCL:
intracellular localization ( 1 ) , molecular association, regulation ( 1 )
Effects of modification on biological processes:
transcription, induced ( 1 )
Inhibit interaction with:
DROSHA (human) ( 1 ) , RNA ( 1 )

References 

1

Gongol B, et al. (2019) Shear stress regulation of miR-93 and miR-484 maturation through nucleolin. Proc Natl Acad Sci U S A 116, 12974-12979
31182601   Curated Info

2

Mertins P, et al. (2016) Proteogenomics connects somatic mutations to signalling in breast cancer. Nature 534, 55-62
27251275   Curated Info

3

Mertins P, et al. (2013) Integrated proteomic analysis of post-translational modifications by serial enrichment. Nat Methods 10, 634-7
23749302   Curated Info

4

Kettenbach AN, et al. (2011) Quantitative phosphoproteomics identifies substrates and functional modules of aurora and polo-like kinase activities in mitotic cells. Sci Signal 4, rs5
21712546   Curated Info