Ser230
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Home > Phosphorylation Site Page: > Ser230  -  HSF1 (human)

Site Information
PkYSRQFsLEHVHGS   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448072

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 16 , 17 ) , electrophoretic mobility shift ( 11 ) , flow cytometry ( 8 ) , immunoprecipitation ( 5 , 8 ) , mass spectrometry ( 12 , 13 , 14 , 15 , 16 ) , mass spectrometry (in vitro) ( 1 ) , microscopy-colocalization with upstream kinase ( 8 ) , mutation of modification site ( 1 , 2 , 5 , 6 , 7 , 8 , 9 ) , peptide sequencing ( 16 ) , phospho-antibody ( 2 , 5 , 8 , 9 , 10 , 11 , 17 ) , phosphoamino acid analysis ( 17 ) , phosphopeptide mapping ( 17 ) , western blotting ( 2 , 5 , 8 , 9 , 10 , 11 )
Disease tissue studied:
colorectal cancer ( 8 ) , colorectal carcinoma ( 8 ) , endometrial cancer ( 4 ) , endometrial adenocarcinoma ( 4 ) , lung cancer ( 9 ) , non-small cell lung cancer ( 9 ) , non-small cell large cell lung carcinoma ( 9 ) , ovarian cancer ( 4 )
Relevant cell line - cell type - tissue:
293 (epithelial) ( 1 , 7 , 8 ) , 3T3 (fibroblast) ( 2 ) , CGL2 (fibroblast) ( 11 ) , HCT116 (intestinal) ( 8 ) , HEC-1B (endometrial) ( 4 ) , HEK293T (epithelial) ( 2 , 11 ) , HeLa (cervical) ( 5 , 6 , 11 , 16 , 17 ) , HTBoA (ovarian) ( 4 ) , Jurkat (T lymphocyte) ( 12 , 13 , 14 , 15 ) , K562 (erythroid) ( 17 ) , LX-2 (hepatic stellate) ( 10 ) , MCAS (ovarian) ( 4 ) , MEF (fibroblast) ( 2 , 6 , 9 ) , myocyte-heart ( 7 ) , NCI-H460 (pulmonary) ( 9 )

Upstream Regulation
Regulatory protein:
PTEN (human) ( 2 )
Putative in vivo kinases:
Akt1 (human) ( 2 ) , Akt2 (human) ( 2 ) , Akt3 (human) ( 2 ) , DAPK1 (human) ( 8 )
Kinases, in vitro:
Akt1 (human) ( 1 , 2 ) , Akt2 (human) ( 2 ) , Akt3 (human) ( 2 ) , CAMK2A (human) ( 17 ) , DAPK1 (human) ( 8 )
Treatments:
2,4-Bis(4-hydroxybenzyl)phenol ( 9 ) , As2O3 ( 11 ) , DAPK_inhibitor ( 8 ) , heat_shock ( 2 , 17 ) , KN-62 ( 5 , 8 ) , KRIBB11 ( 5 ) , LY294002 ( 2 ) , MK-2206 ( 2 ) , TNF ( 8 ) , TRAIL ( 10 ) , virus infection ( 5 )

Downstream Regulation
Effects of modification on HSF1:
activity, induced ( 1 ) , activity, inhibited ( 10 ) , intracellular localization ( 5 ) , molecular association, regulation ( 1 , 2 )
Effects of modification on biological processes:
apoptosis, induced ( 8 ) , carcinogenesis, induced ( 2 ) , cell growth, induced ( 2 ) , transcription, induced ( 1 , 2 , 5 , 8 ) , transcription, inhibited ( 6 )
Induce interaction with:
CDK9 (human) ( 1 ) , DNA ( 2 , 8 )

References 

1

Lu WC, et al. (2022) AKT1 mediates multiple phosphorylation events that functionally promote HSF1 activation. FEBS J
35080342   Curated Info

2

Tang Z, Su KH, Xu M, Dai C (2020) HSF1 physically neutralizes amyloid oligomers to empower overgrowth and bestow neuroprotection. Sci Adv 6
33177089   Curated Info

3

Mun GI, Choi E, Lee Y, Lee YS (2020) Decreased expression of FBXW7 by ERK1/2 activation in drug-resistant cancer cells confers transcriptional activation of MDR1 by suppression of ubiquitin degradation of HSF1. Cell Death Dis
32457290   Curated Info

4

Yasuda K, et al. (2017) Phosphorylation of HSF1 at serine 326 residue is related to the maintenance of gynecologic cancer stem cells through expression of HSP27. Oncotarget 8, 31540-31553
28415561   Curated Info

5

Qiu Y, et al. (2016) Hsp70-1: upregulation via selective phosphorylation of heat shock factor 1 during coxsackieviral infection and promotion of viral replication via the AU-rich element. Cell Mol Life Sci 73, 1067-84
26361762   Curated Info

6

Budzyński MA, Puustinen MC, Joutsen J, Sistonen L (2015) Uncoupling Stress-Inducible Phosphorylation of Heat Shock Factor 1 from Its Activation. Mol Cell Biol 35, 2530-40
25963659   Curated Info

7

Meijering RA, et al. (2015) RhoA Activation Sensitizes Cells to Proteotoxic Stimuli by Abrogating the HSF1-Dependent Heat Shock Response. PLoS One 10, e0133553
26193369   Curated Info

8

Benderska N, et al. (2014) DAPK-HSF1 interaction as a positive-feedback mechanism stimulating TNF-induced apoptosis in colorectal cancer cells. J Cell Sci 127, 5273-87
25380824   Curated Info

9

Yoon T, et al. (2014) 2,4-Bis(4-hydroxybenzyl)phenol Inhibits Heat Shock Transcription Factor 1 and Sensitizes Lung Cancer Cells to Conventional Anticancer Modalities. J Nat Prod 77, 1123-1129
24746225   Curated Info

10

Park SJ, Sohn HY, Park SI (2013) TRAIL regulates collagen production through HSF1-dependent Hsp47 expression in activated hepatic stellate cells. Cell Signal 25, 1635-43
23587601   Curated Info

11

Yih LH, Hsu NC, Kuo HH, Wu YC (2012) Inhibition of the heat shock response by PI103 enhances the cytotoxicity of arsenic trioxide. Toxicol Sci 128, 126-36
22496356   Curated Info

12

Guo A (2012) CST Curation Set: 13866; Year: 2012; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-(Ser/Thr) PKD Substrate Antibody Cat#: 4381, PTMScan(R) Phospho-PKD Substrate Motif (LXRXXpS/pT) Immunoaffinity Beads Cat#: 1986
Curated Info

13

Mulhern D (2012) CST Curation Set: 13758; Year: 2012; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST]
Curated Info

14

Mulhern D (2012) CST Curation Set: 13759; Year: 2012; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST]
Curated Info

15

Mulhern D (2012) CST Curation Set: 13760; Year: 2012; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST]
Curated Info

16

Guettouche T, Boellmann F, Lane WS, Voellmy R (2005) Analysis of phosphorylation of human heat shock factor 1 in cells experiencing a stress. BMC Biochem 6, 4
15760475   Curated Info

17

Holmberg CI, et al. (2001) Phosphorylation of serine 230 promotes inducible transcriptional activity of heat shock factor 1. EMBO J 20, 3800-10
11447121   Curated Info

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