Ser151
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Home > Phosphorylation Site Page: > Ser151  -  GAPDH (human)

Site Information
LkIIsNAsCttNCLA   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 3178959

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 1 ) , mass spectrometry ( 2 , 3 , 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 ) , mutation of modification site ( 1 ) , western blotting ( 1 )
Disease tissue studied:
breast cancer ( 7 ) , liver cancer ( 1 ) , liver adenocarcinoma ( 1 ) , hepatocellular carcinoma ( 1 ) , lung cancer ( 4 , 7 ) , non-small cell lung cancer ( 7 ) , non-small cell lung adenocarcinoma ( 4 )
Relevant cell line - cell type - tissue:
'muscle, skeletal' ( 9 ) , 293 (epithelial) ( 10 ) , BT-20 (breast cell) ( 7 ) , BT-549 (breast cell) ( 7 ) , Calu 6 (pulmonary) ( 7 ) , H2077 (pulmonary) ( 7 ) , H322M (pulmonary) ( 7 ) , HCC366 (pulmonary) ( 7 ) , HCC78 (pulmonary) ( 7 ) , HEK293T (epithelial) ( 1 ) , HeLa (cervical) ( 2 , 3 , 11 ) , HepG2 (hepatic) ( 1 ) , HOP62 (pulmonary) ( 7 ) , Huh7 (hepatic) ( 1 ) , Jurkat (T lymphocyte) ( 5 , 8 ) , K562 (erythroid) ( 6 ) , lung ( 4 ) , MDA-MB-231 (breast cell) ( 7 ) , MDA-MB-468 (breast cell) ( 7 ) , NCI-H1395 (pulmonary) ( 7 ) , NCI-H1648 (pulmonary) ( 7 ) , NCI-H2030 (pulmonary) ( 7 ) , NCI-H322 (pulmonary) ( 7 ) , SK-HEP-1 (endothelial) ( 1 )

Upstream Regulation
Putative in vivo kinases:
VRK2 (human) ( 1 )
Treatments:
IC261 ( 1 )

Downstream Regulation
Effects of modification on GAPDH:
activity, induced ( 1 ) , molecular association, regulation ( 1 )
Effects of modification on biological processes:
cell growth, induced ( 1 ) , cell motility, induced ( 1 )
Induce interaction with:
TRAF2 (human) ( 1 )

References 

1

Lin XT, et al. (2023) Elevated FBXW10 drives hepatocellular carcinoma tumorigenesis via AR-VRK2 phosphorylation-dependent GAPDH ubiquitination in male transgenic mice. Cell Rep 42, 112812
37450367   Curated Info

2

Huang H, et al. (2016) Simultaneous Enrichment of Cysteine-containing Peptides and Phosphopeptides Using a Cysteine-specific Phosphonate Adaptable Tag (CysPAT) in Combination with titanium dioxide (TiO2) Chromatography. Mol Cell Proteomics 15, 3282-3296
27281782   Curated Info

3

Sharma K, et al. (2014) Ultradeep human phosphoproteome reveals a distinct regulatory nature of Tyr and Ser/Thr-based signaling. Cell Rep 8, 1583-94
25159151   Curated Info

4

Schweppe DK, Rigas JR, Gerber SA (2013) Quantitative phosphoproteomic profiling of human non-small cell lung cancer tumors. J Proteomics 91, 286-96
23911959   Curated Info

5

Mertins P, et al. (2013) Integrated proteomic analysis of post-translational modifications by serial enrichment. Nat Methods 10, 634-7
23749302   Curated Info

6

Zhou H, et al. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome. J Proteome Res 12, 260-71
23186163   Curated Info

7

Klammer M, et al. (2012) Phosphosignature predicts dasatinib response in non-small cell lung cancer. Mol Cell Proteomics 11, 651-68
22617229   Curated Info

8

Mulhern D (2011) CST Curation Set: 12709; Year: 2011; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]XP
Curated Info

9

Højlund K, et al. (2009) In vivo phosphoproteome of human skeletal muscle revealed by phosphopeptide enrichment and HPLC-ESI-MS/MS. J Proteome Res 8, 4954-65
19764811   Curated Info

10

Gauci S, et al. (2009) Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach. Anal Chem 81, 4493-501
19413330   Curated Info

11

Dephoure N, et al. (2008) A quantitative atlas of mitotic phosphorylation. Proc Natl Acad Sci U S A 105, 10762-7
18669648   Curated Info

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