Ser55

Site Information
PtsERkVsLFGkRts SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 459763

In vivo Characterization
Methods used to characterize site in vivo:	immunoassay ( 1 , 3 , 4 , 12 ) , immunoprecipitation ( 1 , 2 , 6 ) , mass spectrometry ( 1 , 2 , 6 , 11 , 13 , 15 , 16 , 17 , 18 , 19 , 21 ) , microscopy-colocalization with upstream kinase ( 12 ) , mutation of modification site ( 1 , 2 , 4 ) , phospho-antibody ( 1 , 3 , 4 , 6 , 7 , 8 , 9 , 10 , 12 ) , western blotting ( 1 , 4 , 8 )
Disease tissue studied:	bone cancer ( 4 ) , breast cancer ( 4 , 11 , 16 ) , breast ductal carcinoma ( 11 ) , breast cancer, triple negative ( 11 ) , cervical cancer ( 6 , 7 ) , cervical adenocarcinoma ( 6 , 7 ) , colorectal cancer ( 4 ) , colorectal carcinoma ( 4 ) , lung cancer ( 4 , 16 ) , non-small cell lung cancer ( 4 , 16 ) , non-small cell lung adenocarcinoma ( 4 ) , prostate cancer ( 4 )
Relevant cell line - cell type - tissue:	A549 (pulmonary) ( 4 ) , breast ( 11 ) , BT-20 (breast cell) ( 16 ) , BT-549 (breast cell) ( 16 ) , DLD1 (intestinal) ( 4 ) , DU 145 (prostate cell) ( 4 ) , H2009 (pulmonary) ( 16 ) , H2077 (pulmonary) ( 16 ) , H2887 (pulmonary) ( 16 ) , H322M (pulmonary) ( 16 ) , HCC1937 (breast cell) ( 16 ) , HCC2279 (pulmonary) ( 16 ) , HCC366 (pulmonary) ( 16 ) , HCC78 (pulmonary) ( 16 ) , HCT116 (intestinal) ( 4 ) , HCT15 (intestinal) ( 4 ) , HeLa (cervical) ( 1 , 2 , 3 , 4 , 8 , 9 , 10 , 12 , 18 , 21 ) , HeLa K (cervical) ( 6 ) , HeLa S3 (cervical) [PLK1 (human), knockdown, Tet-inducible PLK1 siRNA] ( 17 ) , HeLa S3 (cervical) ( 7 , 17 , 19 ) , HOP62 (pulmonary) ( 16 ) , hTERT-RPE1 (epithelial) ( 4 , 12 ) , Jurkat (T lymphocyte) ( 13 ) , K562 (erythroid) ( 15 ) , MCF-7 (breast cell) ( 4 , 16 ) , MDA-MB-231 (breast cell) ( 16 ) , MDA-MB-468 (breast cell) ( 16 ) , NCI-H1395 (pulmonary) ( 16 ) , NCI-H1568 (pulmonary) ( 16 ) , NCI-H157 (pulmonary) ( 16 ) , NCI-H1666 (pulmonary) ( 16 ) , NCI-H2030 (pulmonary) ( 16 ) , NCI-H2172 (pulmonary) ( 16 ) , NCI-H322 (pulmonary) ( 16 ) , NCI-H520 (squamous) ( 16 ) , NCI-H647 (pulmonary) ( 16 ) , PC9 (pulmonary) ( 16 ) , PtK1 (epithelial) ( 6 ) , RPE-1 (retinal) ( 4 ) , TIG-3 TD ( 4 ) , U2OS (bone cell) ( 4 )

Upstream Regulation
Regulatory protein:	BOD1 (human) ( 7 ) , INCENP (human) ( 4 ) , KIF18A (human) ( 4 ) , PPP6C (human) ( 1 ) , TPX2 (human) ( 1 , 4 ) , TPX2 (mouse) ( 4 )
Putative in vivo kinases:	AurA (human) ( 1 , 4 , 8 ) , AurB (human) ( 9 )
Kinases, in vitro:	AurA (human) ( 6 , 8 ) , AurB (human) ( 6 , 20 )
Treatments:	AZD1152 ( 1 , 4 ) , BI2536 ( 18 ) , BTB-1 ( 4 ) , calyculin_A ( 1 , 4 ) , hyper-intrakinetochore_stretch ( 10 ) , IAA ( 4 ) , MKLP2i3 ( 3 ) , MLN8054 ( 6 , 18 ) , MLN8237 ( 1 , 4 ) , nocodazole ( 1 , 2 ) , siRNA ( 1 ) , STLC ( 2 ) , TAME ( 2 ) , taxol ( 4 ) , UMK57 ( 4 ) , ZK-Thiazolidinone ( 17 ) , ZM447439 ( 1 , 6 )

Downstream Regulation
Effects of modification on biological processes:	cell cycle regulation ( 1 , 3 , 20 ) , chromatin organization, altered ( 3 , 4 ) , cytoskeletal reorganization ( 2 )

References
1	Sobajima T, et al. (2023) PP6 regulation of Aurora A-TPX2 limits NDC80 phosphorylation and mitotic spindle size. J Cell Biol 222 36897279 Curated Info
2	Kucharski TJ, et al. (2022) Small changes in phospho-occupancy at the kinetochore-microtubule interface drive mitotic fidelity. J Cell Biol 221 35878017 Curated Info
3	Schrock MS, et al. (2022) MKLP2 functions in early mitosis to ensure proper chromosome congression. J Cell Sci 135 35638575 Curated Info
4	Iemura K, et al. (2021) Chromosome oscillation promotes Aurora A-dependent Hec1 phosphorylation and mitotic fidelity. J Cell Biol 220 33988677 Curated Info
5	Zhao G, et al. (2018) Dynamic acetylation of the kinetochore-associated protein HEC1 ensures accurate microtubule-kinetochore attachment. J Biol Chem 30409912 Curated Info
6	DeLuca KF, et al. (2018) Aurora A kinase phosphorylates Hec1 to regulate metaphase kinetochore-microtubule dynamics. J Cell Biol 217, 163-177 29187526 Curated Info
7	Schleicher K, et al. (2017) The Ndc80 complex targets Bod1 to human mitotic kinetochores. Open Biol 7 29142109 Curated Info
8	Ye AA, et al. (2015) Aurora A Kinase Contributes to a Pole-Based Error Correction Pathway. Curr Biol 25, 1842-51 26166783 Curated Info
9	Li S, et al. (2015) Spatial Compartmentalization Specializes the Function of Aurora A and Aurora B. J Biol Chem 290, 17546-58 25987563 Curated Info
10	Suzuki A, et al. (2014) The Architecture of CCAN Proteins Creates a Structural Integrity to Resist Spindle Forces and Achieve Proper Intrakinetochore Stretch. Dev Cell 30, 717-30 25268173 Curated Info
11	Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704 24719451 Curated Info
12	Caldas GV, DeLuca KF, DeLuca JG (2013) KNL1 facilitates phosphorylation of outer kinetochore proteins by promoting Aurora B kinase activity. J Cell Biol 203, 957-69 24344188 Curated Info
13	Mertins P, et al. (2013) Integrated proteomic analysis of post-translational modifications by serial enrichment. Nat Methods 10, 634-7 23749302 Curated Info
14	Shiromizu T, et al. (2013) Identification of missing proteins in the neXtProt database and unregistered phosphopeptides in the PhosphoSitePlus database as part of the Chromosome-centric Human Proteome Project. J Proteome Res 12, 2414-21 23312004 Curated Info
15	Zhou H, et al. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome. J Proteome Res 12, 260-71 23186163 Curated Info
16	Klammer M, et al. (2012) Phosphosignature predicts dasatinib response in non-small cell lung cancer. Mol Cell Proteomics 11, 651-68 22617229 Curated Info
17	Santamaria A, et al. (2011) The Plk1-dependent phosphoproteome of the early mitotic spindle. Mol Cell Proteomics 10, M110.004457 20860994 Curated Info
18	Kettenbach AN, et al. (2011) Quantitative phosphoproteomics identifies substrates and functional modules of aurora and polo-like kinase activities in mitotic cells. Sci Signal 4, rs5 21712546 Curated Info
19	Malik R, et al. (2009) Quantitative analysis of the human spindle phosphoproteome at distinct mitotic stages. J Proteome Res 8, 4553-63 19691289 Curated Info
20	DeLuca JG, et al. (2006) Kinetochore microtubule dynamics and attachment stability are regulated by Hec1. Cell 127, 969-82 17129782 Curated Info
21	Nousiainen M, et al. (2006) Phosphoproteome analysis of the human mitotic spindle. Proc Natl Acad Sci U S A 103, 5391-6 16565220 Curated Info