Ser1524
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Home > Phosphorylation Site Page: > Ser1524  -  BRCA1 (human)

Site Information
LQNRNYPsQEELIKV   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448038

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 25 , 27 ) , electrophoretic mobility shift ( 27 ) , immunoprecipitation ( 23 ) , mass spectrometry ( 1 , 4 , 5 , 6 , 7 , 9 , 10 , 12 , 13 , 14 , 15 , 16 , 17 , 21 , 27 ) , mutation of modification site ( 3 , 19 , 20 , 25 , 27 ) , phospho-antibody ( 19 , 22 , 23 , 24 , 26 ) , western blotting ( 3 , 19 , 23 )
Disease tissue studied:
bone cancer ( 23 ) , brain cancer ( 19 ) , glioblastoma ( 19 ) , glioma ( 19 ) , breast cancer ( 3 , 6 , 10 , 19 , 24 ) , breast adenocarcinoma ( 24 ) , breast ductal carcinoma ( 3 , 6 , 24 ) , HER2 positive breast cancer ( 1 ) , luminal A breast cancer ( 1 ) , luminal B breast cancer ( 1 ) , breast cancer, surrounding tissue ( 1 ) , breast cancer, triple negative ( 1 , 3 ) , cervical cancer ( 17 ) , cervical adenocarcinoma ( 17 ) , leukemia ( 12 ) , acute myelogenous leukemia ( 12 ) , lung cancer ( 10 ) , non-small cell lung cancer ( 10 ) , melanoma skin cancer ( 4 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Regulatory protein:
claspin (human) ( 23 )
Putative in vivo kinases:
ATM (human) ( 26 ) , ATR (human) ( 26 )
Kinases, in vitro:
ATM (human) ( 27 )
Treatments:
2-deoxyglucose ( 18 ) , DIM ( 19 ) , ionizing_radiation ( 23 , 24 , 25 , 26 , 27 ) , siRNA ( 23 ) , UV ( 23 , 26 )

Downstream Regulation
Effects of modification on BRCA1:
molecular association, regulation ( 23 , 27 ) , phosphorylation ( 22 )
Effects of modification on biological processes:
cell cycle regulation ( 3 , 25 ) , DNA repair, altered ( 3 )
Induce interaction with:
ATM (human) ( 27 ) , claspin (human) ( 23 )

References 

1

Mertins P, et al. (2016) Proteogenomics connects somatic mutations to signalling in breast cancer. Nature 534, 55-62
27251275   Curated Info

2

Boeing S, et al. (2016) Multiomic Analysis of the UV-Induced DNA Damage Response. Cell Rep 15, 1597-1610
27184836   Curated Info

3

Beckta JM, et al. (2015) Mutation of the BRCA1 SQ-cluster results in aberrant mitosis, reduced homologous recombination, and a compensatory increase in non-homologous end joining. Oncotarget 6, 27674-87
26320175   Curated Info

4

Stuart SA, et al. (2015) A Phosphoproteomic Comparison of B-RAFV600E and MKK1/2 Inhibitors in Melanoma Cells. Mol Cell Proteomics 14, 1599-615
25850435   Curated Info

5

Sharma K, et al. (2014) Ultradeep human phosphoproteome reveals a distinct regulatory nature of Tyr and Ser/Thr-based signaling. Cell Rep 8, 1583-94
25159151   Curated Info

6

Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704
24719451   Curated Info

7

Mertins P, et al. (2013) Integrated proteomic analysis of post-translational modifications by serial enrichment. Nat Methods 10, 634-7
23749302   Curated Info

8

Shiromizu T, et al. (2013) Identification of missing proteins in the neXtProt database and unregistered phosphopeptides in the PhosphoSitePlus database as part of the Chromosome-centric Human Proteome Project. J Proteome Res 12, 2414-21
23312004   Curated Info

9

Zhou H, et al. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome. J Proteome Res 12, 260-71
23186163   Curated Info

10

Klammer M, et al. (2012) Phosphosignature predicts dasatinib response in non-small cell lung cancer. Mol Cell Proteomics 11, 651-68
22617229   Curated Info

11

Beli P, et al. (2012) Proteomic Investigations Reveal a Role for RNA Processing Factor THRAP3 in the DNA Damage Response. Mol Cell 46, 212-25
22424773   Curated Info

12

Weber C, Schreiber TB, Daub H (2012) Dual phosphoproteomics and chemical proteomics analysis of erlotinib and gefitinib interference in acute myeloid leukemia cells. J Proteomics 75, 1343-56
22115753   Curated Info

13

Mulhern D (2011) CST Curation Set: 12680; Year: 2011; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: (F/Y/M)Xp[ST](L/I/M)
Curated Info

14

Kettenbach AN, et al. (2011) Quantitative phosphoproteomics identifies substrates and functional modules of aurora and polo-like kinase activities in mitotic cells. Sci Signal 4, rs5
21712546   Curated Info

15

Christensen GL, et al. (2010) Quantitative phosphoproteomics dissection of seven-transmembrane receptor signaling using full and biased agonists. Mol Cell Proteomics 9, 1540-53
20363803   Curated Info

16

Possemato A (2010) CST Curation Set: 9070; Year: 2010; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: (F/Y)XpS
Curated Info

17

Olsen JV, et al. (2010) Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis. Sci Signal 3, ra3
20068231   Curated Info

18

Zhong D, et al. (2009) The Glycolytic Inhibitor 2-Deoxyglucose Activates Multiple Prosurvival Pathways through IGF1R. J Biol Chem 284, 23225-33
19574224   Curated Info

19

Fan S, et al. (2009) Low concentrations of diindolylmethane, a metabolite of indole-3-carbinol, protect against oxidative stress in a BRCA1-dependent manner. Cancer Res 69, 6083-91
19622773   Curated Info

20

Zhang F, et al. (2009) PALB2 links BRCA1 and BRCA2 in the DNA-damage response. Curr Biol 19, 524-9
19268590   Curated Info

21

Matsuoka S, et al. (2007) ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage. Science 316, 1160-6
17525332   Curated Info

22

Fabbro M, et al. (2004) BRCA1-BARD1 complexes are required for p53Ser-15 phosphorylation and a G1/S arrest following ionizing radiation-induced DNA damage. J Biol Chem 279, 31251-8
15159397   Curated Info

23

Lin SY, Li K, Stewart GS, Elledge SJ (2004) Human Claspin works with BRCA1 to both positively and negatively regulate cell proliferation. Proc Natl Acad Sci U S A 101, 6484-9
15096610   Curated Info

24

Okada S, Ouchi T (2003) Cell cycle differences in DNA damage-induced BRCA1 phosphorylation affect its subcellular localization. J Biol Chem 278, 2015-20
12427729   Curated Info

25

Xu B, O'Donnell AH, Kim ST, Kastan MB (2002) Phosphorylation of serine 1387 in Brca1 is specifically required for the Atm-mediated S-phase checkpoint after ionizing irradiation. Cancer Res 62, 4588-91
12183412   Curated Info

26

Gatei M, et al. (2001) Ataxia telangiectasia mutated (ATM) kinase and ATM and Rad3 related kinase mediate phosphorylation of Brca1 at distinct and overlapping sites. In vivo assessment using phospho-specific antibodies. J Biol Chem 276, 17276-80
11278964   Curated Info

27

Cortez D, Wang Y, Qin J, Elledge SJ (1999) Requirement of ATM-dependent phosphorylation of brca1 in the DNA damage response to double-strand breaks. Science 286, 1162-6
10550055   Curated Info