Ser1423
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Home > Phosphorylation Site Page: > Ser1423  -  BRCA1 (human)

Site Information
AVLEQHGsQPSNSYP   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448036

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 9 , 14 ) , electrophoretic mobility shift ( 13 , 14 ) , mass spectrometry ( 3 , 14 ) , mutation of modification site ( 1 , 4 , 5 , 9 , 11 , 12 , 14 ) , phospho-antibody ( 2 , 4 , 6 , 7 , 8 , 10 , 12 , 13 ) , western blotting ( 1 , 2 , 4 , 6 , 13 )
Disease tissue studied:
ataxia-telangiectasia ( 6 , 13 ) , brain cancer ( 4 ) , glioblastoma ( 4 ) , glioma ( 4 ) , breast cancer ( 1 , 2 , 4 , 8 ) , breast adenocarcinoma ( 8 ) , breast ductal carcinoma ( 1 , 8 ) , breast cancer, triple negative ( 1 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Regulatory protein:
NBS1 (human) ( 2 ) , RAP80 (human) ( 2 )
Putative in vivo kinases:
ATM (human) ( 10 , 13 ) , ATR (human) ( 10 , 12 )
Kinases, in vitro:
ATM (human) ( 13 , 14 ) , ATR (human) ( 12 )
Treatments:
aphidicolin ( 12 ) , DIM ( 4 ) , etoposide ( 6 ) , Go_6976 ( 6 ) , hydroxyurea ( 6 , 12 ) , ionizing_radiation ( 8 , 9 , 10 , 12 , 13 , 14 ) , UCN-01 ( 6 ) , UV ( 10 , 12 )

Downstream Regulation
Effects of modification on BRCA1:
molecular association, regulation ( 14 ) , phosphorylation ( 7 )
Effects of modification on biological processes:
cell cycle regulation ( 1 , 2 , 9 , 11 ) , DNA repair, altered ( 1 )
Induce interaction with:
ATM (human) ( 14 )

Disease / Diagnostics Relevance
Relevant diseases:
ataxia-telangiectasia ( 13 )

References 

1

Beckta JM, et al. (2015) Mutation of the BRCA1 SQ-cluster results in aberrant mitosis, reduced homologous recombination, and a compensatory increase in non-homologous end joining. Oncotarget 6, 27674-87
26320175   Curated Info

2

Goldstein M, Kastan MB (2015) Repair versus Checkpoint Functions of BRCA1 Are Differentially Regulated by Site of Chromatin Binding. Cancer Res 75, 2699-707
25939603   Curated Info

3

Mulhern D (2011) CST Curation Set: 12712; Year: 2011; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]XP
Curated Info

4

Fan S, et al. (2009) Low concentrations of diindolylmethane, a metabolite of indole-3-carbinol, protect against oxidative stress in a BRCA1-dependent manner. Cancer Res 69, 6083-91
19622773   Curated Info

5

Zhang F, et al. (2009) PALB2 links BRCA1 and BRCA2 in the DNA-damage response. Curr Biol 19, 524-9
19268590   Curated Info

6

Leung-Pineda V, Ryan CE, Piwnica-Worms H (2006) Phosphorylation of Chk1 by ATR is antagonized by a Chk1-regulated protein phosphatase 2A circuit. Mol Cell Biol 26, 7529-38
17015476   Curated Info

7

Fabbro M, et al. (2004) BRCA1-BARD1 complexes are required for p53Ser-15 phosphorylation and a G1/S arrest following ionizing radiation-induced DNA damage. J Biol Chem 279, 31251-8
15159397   Curated Info

8

Okada S, Ouchi T (2003) Cell cycle differences in DNA damage-induced BRCA1 phosphorylation affect its subcellular localization. J Biol Chem 278, 2015-20
12427729   Curated Info

9

Xu B, O'Donnell AH, Kim ST, Kastan MB (2002) Phosphorylation of serine 1387 in Brca1 is specifically required for the Atm-mediated S-phase checkpoint after ionizing irradiation. Cancer Res 62, 4588-91
12183412   Curated Info

10

Gatei M, et al. (2001) Ataxia telangiectasia mutated (ATM) kinase and ATM and Rad3 related kinase mediate phosphorylation of Brca1 at distinct and overlapping sites. In vivo assessment using phospho-specific antibodies. J Biol Chem 276, 17276-80
11278964   Curated Info

11

Xu B, Kim ST, Kastan MB (2001) Involvement of Brca1 in S-phase and G(2)-phase checkpoints after ionizing irradiation. Mol Cell Biol 21, 3445-50
11313470   Curated Info

12

Tibbetts RS, et al. (2000) Functional interactions between BRCA1 and the checkpoint kinase ATR during genotoxic stress. Genes Dev 14, 2989-3002
11114888   Curated Info

13

Gatei M, et al. (2000) Role for ATM in DNA damage-induced phosphorylation of BRCA1. Cancer Res 60, 3299-304
10866324   Curated Info

14

Cortez D, Wang Y, Qin J, Elledge SJ (1999) Requirement of ATM-dependent phosphorylation of brca1 in the DNA damage response to double-strand breaks. Science 286, 1162-6
10550055   Curated Info