Tyr823
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Home > Phosphorylation Site Page: > Tyr823  -  Kit (human)

Site Information
DIKNDsNyVVKGNAR   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448018
Associated spectra:  2 CST

In vivo Characterization
Methods used to characterize site in vivo:
flow cytometry ( 3 ) , immunoprecipitation ( 3 ) , mass spectrometry ( 1 , 2 , 4 , 5 , 7 , 8 , 9 , 10 , 11 , 12 ) , mass spectrometry (in vitro) ( 6 ) , mutation of modification site ( 3 , 13 ) , phospho-antibody ( 3 ) , western blotting ( 3 )
Disease tissue studied:
gastric cancer ( 2 , 7 , 9 , 10 ) , gastric carcinoma ( 10 ) , leukemia ( 3 , 11 , 12 ) , acute myelogenous leukemia ( 3 , 11 , 12 ) , acute myeloblastic leukemia, with granulocytic maturation (M2) ( 3 ) , liver cancer ( 8 ) , neuroblastoma ( 1 , 4 , 5 )
Relevant cell line - cell type - tissue:
BaF3 ('B lymphocyte, precursor') ( 3 ) , CHRF (megakaryocyte) ( 11 ) , COS7 (fibroblast) ( 3 ) , GIST-T1 (gastrointestinal) ( 2 ) , IC2 (mast) ( 13 ) , Kasumi-2 (B lymphocyte) ( 3 ) , liver ( 8 ) , M-07e (myeloid) ( 3 , 12 ) , SK-N-BE(2) (neural crest) ( 1 , 4 , 5 ) , stomach ( 7 , 9 , 10 )

Upstream Regulation
Kinases, in vitro:
Kit (human) ( 6 )
Treatments:
imatinib ( 2 ) , SCF ( 3 )

Downstream Regulation
Effects of modification on Kit:
phosphorylation ( 3 ) , protein stabilization ( 3 ) , receptor internalization, inhibited ( 3 )
Effects of modification on biological processes:
apoptosis, inhibited ( 3 ) , cell growth, induced ( 3 ) , signaling pathway regulation ( 3 )

References 

1

Palacios-Moreno J, et al. (2015) Neuroblastoma Tyrosine Kinase Signaling Networks Involve FYN and LYN in Endosomes and Lipid Rafts. PLoS Comput Biol 11, e1004130
25884760   Curated Info

2

Takahashi T, et al. (2013) New findings of kinase switching in gastrointestinal stromal tumor under imatinib using phosphoproteomic analysis. Int J Cancer 133, 2737-43
23716303   Curated Info

3

Agarwal S, Kazi JU, Rönnstrand L (2013) Phosphorylation of the Activation Loop Tyrosine 823 in c-Kit Is Crucial for Cell Survival and Proliferation. J Biol Chem 288, 22460-8
23803604   Curated Info

4

Guo A (2010) CST Curation Set: 10551; Year: 2010; Biosample/Treatment: cell line, SKNBE(2)/NGF; Disease: neuroblastoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

5

Guo A (2010) CST Curation Set: 10549; Year: 2010; Biosample/Treatment: cell line, SKNBE(2)/NGF; Disease: neuroblastoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

6

DiNitto JP, et al. (2010) Function of activation loop tyrosine phosphorylation in the mechanism of c-Kit auto-activation and its implication in sunitinib resistance. J Biochem 147, 601-9
20147452   Curated Info

7

Ren H (2009) CST Curation Set: 8060; Year: 2009; Biosample/Treatment: tissue, gastrointestinal tract/untreated; Disease: gastric cancer; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

8

Gu T (2009) CST Curation Set: 7526; Year: 2009; Biosample/Treatment: tissue, liver/untreated; Disease: liver cancer; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

9

Ren H (2008) CST Curation Set: 4091; Year: 2008; Biosample/Treatment: tissue, gastrointestinal tract/untreated; Disease: gastric cancer; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

10

Ren H (2008) CST Curation Set: 3988; Year: 2008; xenograft, Biosample/Treatment: tissue, gastrointestinal tract/untreated; Disease: gastric carcinoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

11

Gu T (2005) CST Curation Set: 842; Year: 2005; Biosample/Treatment: cell line, CHRF/-; Disease: acute myelogenous leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

12

Gu T (2005) CST Curation Set: 723; Year: 2005; Biosample/Treatment: cell line, M-07e/-; Disease: acute myelogenous leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

13

Koma Y, et al. (2005) Distinct role for c-kit receptor tyrosine kinase and SgIGSF adhesion molecule in attachment of mast cells to fibroblasts. Lab Invest 85, 426-35
15654360   Curated Info