Tyr785
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Home > Phosphorylation Site Page: > Tyr785  -  ITGB3 (human)

Site Information
stFTNItyRGT____   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448013

In vivo Characterization
Methods used to characterize site in vivo:
2D analysis ( 20 ) , [32P] bio-synthetic labeling ( 18 ) , immunoprecipitation ( 1 ) , mass spectrometry ( 3 , 5 , 8 , 12 , 13 ) , mutation of modification site ( 1 , 3 , 4 , 9 , 15 , 16 , 18 , 19 ) , peptide sequencing ( 18 ) , phospho-antibody ( 1 , 2 , 7 , 10 , 11 , 14 , 17 , 20 ) , western blotting ( 1 , 2 , 7 , 10 , 11 , 14 , 20 )
Disease tissue studied:
bone cancer ( 19 ) , leukemia ( 12 , 13 ) , acute myelogenous leukemia ( 12 , 13 )
Relevant cell line - cell type - tissue:
'muscle, smooth' ( 16 ) , CHO-K1 (fibroblast) ( 4 ) , CHRF (megakaryocyte) ( 13 ) , E.coli (bacterial) ( 9 ) , EC (endothelial) ( 3 ) , HCEC (epithelial) ( 2 ) , HOS (bone cell) ( 19 ) , IEC-6 (epithelial) ( 11 ) , Jurkat (T lymphocyte) ( 5 ) , K562 (erythroid) ( 15 , 17 , 18 ) , myeloid-bone marrow ( 12 ) , platelet-blood ( 7 , 8 , 10 , 14 , 20 )

Upstream Regulation
Regulatory protein:
TGM2 (human) ( 2 )
Putative in vivo kinases:
Src (human) ( 7 )
Treatments:
2-deoxyglucose ( 6 ) , A23187 ( 10 ) , cell_adhesion ( 11 ) , eflornithine ( 11 ) , peptide inhibitor ( 7 ) , putrescine ( 11 ) , RGD ( 18 ) , RGDS ( 11 ) , spermine ( 11 ) , thrombin ( 7 , 10 ) , vanadate ( 10 )

Downstream Regulation
Effects of modification on ITGB3:
activity, inhibited ( 4 , 10 ) , intracellular localization ( 9 ) , molecular association, regulation ( 4 , 9 , 20 ) , protein processing ( 10 )
Effects of modification on biological processes:
cell adhesion, altered ( 15 , 19 ) , cytoskeletal reorganization ( 17 )
Induce interaction with:
GRB2 (human) ( 20 ) , SHC1 (human) ( 20 ) , tensin 1 (human) ( 9 )
Inhibit interaction with:
kindlin-2 (human) ( 4 )

References 

1

Simpson MA, et al. (2015) Direct interactions with the integrin β1 cytoplasmic tail activate the Abl2/Arg kinase. J Biol Chem 290, 8360-72
25694433   Curated Info

2

Tong L, et al. (2013) Molecular mechanism of transglutaminase-2 in corneal epithelial migration and adhesion. Biochim Biophys Acta 1833, 1304-15
23466867   Curated Info

3

Deshmukh L, et al. (2011) Tyrosine phosphorylation as a conformational switch: a case study of integrin β3 cytoplasmic tail. J Biol Chem 286, 40943-53
21956114   Curated Info

4

Bledzka K, et al. (2010) Tyrosine phosphorylation of integrin beta3 regulates kindlin-2 binding and integrin activation. J Biol Chem 285, 30370-4
20702409   Curated Info

5

Guo A (2010) CST Curation Set: 9180; Year: 2010; Biosample/Treatment: cell line, Jurkat/pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

6

Zhong D, et al. (2009) The Glycolytic Inhibitor 2-Deoxyglucose Activates Multiple Prosurvival Pathways through IGF1R. J Biol Chem 284, 23225-33
19574224   Curated Info

7

Su X, et al. (2008) RGT, a synthetic peptide corresponding to the integrin beta 3 cytoplasmic C-terminal sequence, selectively inhibits outside-in signaling in human platelets by disrupting the interaction of integrin alpha IIb beta 3 with Src kinase. Blood 112, 592-602
18398066   Curated Info

8

Zahedi RP, et al. (2008) Phosphoproteome of resting human platelets. J Proteome Res 7, 526-34
18088087   Curated Info

9

McCleverty CJ, Lin DC, Liddington RC (2007) Structure of the PTB domain of tensin1 and a model for its recruitment to fibrillar adhesions. Protein Sci 16, 1223-9
17473008   Curated Info

10

Xi X, et al. (2006) Tyrosine phosphorylation of the integrin beta 3 subunit regulates beta 3 cleavage by calpain. J Biol Chem 281, 29426-30
16935858   Curated Info

11

Bhattacharya S, Ray RM, Johnson LR (2006) Integrin beta3-mediated Src activation regulates apoptosis in IEC-6 cells via Akt and STAT3. Biochem J 397, 437-47
16669788   Curated Info

12

Gu T (2006) CST Curation Set: 1457; Year: 2006; Biosample/Treatment: bone marrow, myeloid/untreated; Disease: acute myelogenous leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

13

Gu T (2005) CST Curation Set: 842; Year: 2005; Biosample/Treatment: cell line, CHRF/-; Disease: acute myelogenous leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

14

Vijayan KV, et al. (2005) The Pro33 isoform of integrin beta3 enhances outside-in signaling in human platelets by regulating the activation of serine/threonine phosphatases. J Biol Chem 280, 21756-62
15826939   Curated Info

15

Gao C, Schaefer E, Lakkis M, Blystone SD (2005) Beta3 tyrosine phosphorylation and alphavbeta3-mediated adhesion are required for Vav1 association and Rho activation in leukocytes. J Biol Chem 280, 15422-9
15699036   Curated Info

16

Courter DL, Lomas L, Scatena M, Giachelli CM (2005) Src kinase activity is required for integrin alphaVbeta3-mediated activation of nuclear factor-kappaB. J Biol Chem 280, 12145-51
15695822   Curated Info

17

Chandhoke SK, et al. (2004) Beta 3 integrin phosphorylation is essential for Arp3 organization into leukocyte alpha V beta 3-vitronectin adhesion contacts. J Cell Sci 117, 1431-41
14996908   Curated Info

18

Blystone SD (2002) Kinetic regulation of beta 3 integrin tyrosine phosphorylation. J Biol Chem 277, 46886-90
12359711   Curated Info

19

Datta A, Huber F, Boettiger D (2002) Phosphorylation of beta3 integrin controls ligand binding strength. J Biol Chem 277, 3943-9
11723131   Curated Info

20

Law DA, Nannizzi-Alaimo L, Phillips DR (1996) Outside-in integrin signal transduction. Alpha IIb beta 3-(GP IIb IIIa) tyrosine phosphorylation induced by platelet aggregation. J Biol Chem 271, 10811-5
8631894   Curated Info