Thr494
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Home > Phosphorylation Site Page: > Thr494  -  eNOS (mouse)

Site Information
AGITRKKtFKEVANA   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 447885

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 6 ) , phospho-antibody ( 2 , 3 , 4 , 5 , 6 ) , western blotting ( 2 , 3 , 4 , 5 )
Relevant cell line - cell type - tissue:
aorta ( 2 , 4 , 5 ) , BAEC (endothelial) ( 6 ) , heart ( 3 ) , HUVEC (endothelial) ( 6 ) , myocyte-heart ( 6 )

Upstream Regulation
Regulatory protein:
AMPKA2 (mouse) ( 3 ) , APOE (mouse) ( 2 ) , FKBP12 (mouse) ( 5 ) , FKBP14 (mouse) ( 5 )
Treatments:
ACh ( 2 ) , FGF2 ( 6 ) , Go_6976 ( 5 ) , ischemia/reperfusion ( 3 ) , metformin ( 3 ) , PGE2 ( 4 ) , rapamycin ( 5 ) , ryanodine ( 5 )

References 

1

Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704
24719451   Curated Info

2

Capettini LS, et al. (2011) Decreased production of neuronal NOS-derived hydrogen peroxide contributes to endothelial dysfunction in atherosclerosis. Br J Pharmacol 164, 1738-48
21615722   Curated Info

3

Calvert JW, et al. (2008) Acute metformin therapy confers cardioprotection against myocardial infarction via AMPK-eNOS-mediated signaling. Diabetes 57, 696-705
18083782   Curated Info

4

Hristovska AM, et al. (2007) Prostaglandin E2 induces vascular relaxation by E-prostanoid 4 receptor-mediated activation of endothelial nitric oxide synthase. Hypertension 50, 525-30
17635857   Curated Info

5

Long C, et al. (2007) FK506 binding protein 12/12.6 depletion increases endothelial nitric oxide synthase threonine 495 phosphorylation and blood pressure. Hypertension 49, 569-76
17261647   Curated Info

6

Partovian C, et al. (2005) PKCalpha activates eNOS and increases arterial blood flow in vivo. Circ Res 97, 482-7
16081872   Curated Info