Ser373
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage PhosphoSitePlus® v6.7.5
Powered by Cell Signaling Technology
Home > Phosphorylation Site Page: > Ser373  -  GJA1 (human)

Site Information
RAssRAssRPRPDDL   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 459209

In vivo Characterization
Methods used to characterize site in vivo:
electrophoretic mobility shift ( 8 ) , immunoassay ( 2 ) , immunoprecipitation ( 3 , 6 , 7 ) , mass spectrometry ( 3 , 5 , 9 ) , mutation of modification site ( 3 , 6 , 7 , 8 ) , phospho-antibody ( 1 , 2 , 4 , 7 ) , western blotting ( 1 , 2 , 3 , 4 , 7 )
Disease tissue studied:
lung cancer ( 9 ) , non-small cell lung cancer ( 9 ) , ovarian cancer ( 5 )
Relevant cell line - cell type - tissue:
293 (epithelial) ( 3 ) , H2077 (pulmonary) ( 9 ) , H322M (pulmonary) ( 9 ) , HCC1359 (pulmonary) ( 9 ) , HCC4006 (pulmonary) ( 9 ) , HCC827 (pulmonary) ( 9 ) , HOP62 (pulmonary) ( 9 ) , HPMEC (pulmonary) ( 1 ) , IAR20 (epithelial) ( 3 ) , LA-25 (fibroblast) ( 2 ) , LCLC-103H (pulmonary) ( 9 ) , MDCK (epithelial) ( 7 ) , MLO-Y4 (osteoblast) ( 6 ) , NCI-H1395 (pulmonary) ( 9 ) , NCI-H2030 (pulmonary) ( 9 ) , NCI-H322 (pulmonary) ( 9 ) , NRK (fibroblast) ( 2 ) , ovary ( 5 ) , stromal ( 4 ) , trophoblast ( 3 )

Upstream Regulation
Regulatory protein:
GJA1 (human) ( 1 )
Putative in vivo kinases:
Akt1 (human) ( 6 ) , PKACA (human) ( 3 )
Kinases, in vitro:
Akt1 (human) ( 6 ) , PKACA (human) ( 3 , 8 )
Treatments:
Akt_inhibitor_IV ( 6 ) , bafilomycin_A ( 2 ) , brefeldin_A ( 2 ) , cAMP_analog ( 3 ) , cycloheximide ( 2 ) , deoxygenation ( 7 ) , hypoxia ( 7 ) , mechanical stress ( 6 ) , MG132 ( 2 ) , phorbol_ester ( 7 ) , scratch wound ( 7 ) , vanadate ( 2 )

Downstream Regulation
Effects of modification on GJA1:
activity, induced ( 6 ) , intracellular localization ( 3 ) , molecular association, regulation ( 6 , 7 ) , protein conformation ( 8 )
Induce interaction with:
ITGA5 (mouse) ( 6 )
Inhibit interaction with:
ZO1 (human) ( 7 )

References 

1

Zhou Z, et al. (2022) Connexin 43 overexpression induces lung cancer angiogenesis following phosphorylation at Ser279 in its C-terminus. Oncol Lett 24, 293
35949588   Curated Info

2

Solan JL, Lampe PD (2020) Src Regulation of Cx43 Phosphorylation and Gap Junction Turnover. Biomolecules 10
33255329   Curated Info

3

Dukic AR, et al. (2017) Ezrin-anchored PKA phosphorylates serine 369 and 373 on connexin 43 to enhance gap junction assembly, communication, and cell fusion. Biochem J
29259079   Curated Info

4

Balla P, et al. (2015) Prognostic impact of reduced connexin43 expression and gap junction coupling of neoplastic stromal cells in giant cell tumor of bone. PLoS One 10, e0125316
25933380   Curated Info

5

Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704
24719451   Curated Info

6

Batra N, et al. (2014) Direct regulation of osteocytic connexin 43 hemichannels through AKT kinase activated by mechanical stimulation. J Biol Chem 289, 10582-91
24563481   Curated Info

7

Dunn CA, Lampe PD (2014) Injury-triggered Akt phosphorylation of Cx43: a ZO-1-driven molecular switch that regulates gap junction size. J Cell Sci 127, 455-64
24213533   Curated Info

8

Grosely R, et al. (2013) Effects of Phosphorylation on the Structure and Backbone Dynamics of the Intrinsically Disordered Connexin43 C-terminal Domain. J Biol Chem 288, 24857-70
23828237   Curated Info

9

Klammer M, et al. (2012) Phosphosignature predicts dasatinib response in non-small cell lung cancer. Mol Cell Proteomics 11, 651-68
22617229   Curated Info

Developed with grants from  NIH Logo and literature mining with Linguamatics  I2E Logo Creative Commons License PhosphoSite, created by Cell Signaling Technology is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License.

©2003-2024 Cell Signaling Technology, Inc.