Ser373
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Home > Phosphorylation Site Page: > Ser373  -  GJA1 (rat)

Site Information
RAssRAssRPRPDDL   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 459209

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 8 ) , immunoprecipitation ( 1 ) , mass spectrometry ( 7 ) , mass spectrometry (in vitro) ( 3 ) , mutation of modification site ( 1 , 6 , 8 ) , phospho-antibody ( 2 , 4 ) , phosphoamino acid analysis ( 8 ) , phosphopeptide mapping ( 8 ) , western blotting ( 1 , 2 , 4 )
Relevant cell line - cell type - tissue:
astrocyte-'brain, cerebrum' ( 2 ) , granulosa ( 8 ) , heart ( 7 ) , HeLa (cervical) ( 1 , 8 ) , MDCK (epithelial) ( 1 ) , preovulatory follicle granulosa ( 6 ) , Rat1 (fibroblast) ( 4 )

Upstream Regulation
Putative in vivo kinases:
Akt3 (rat) ( 4 ) , PKACA (human) ( 6 )
Kinases, in vitro:
Akt1 (human) ( 4 , 5 ) , CAMK2A (human) ( 3 ) , PKACA (human) ( 6 ) , PKCA (human) ( 7 ) , PKCE (human) ( 7 )
Treatments:
EGF ( 4 ) , H-89 ( 6 ) , PD98059 ( 6 ) , urofollitropin ( 6 , 8 )

Downstream Regulation
Effects of modification on GJA1:
activity, induced ( 8 ) , intracellular localization ( 1 ) , molecular association, regulation ( 1 , 4 , 5 ) , protein degradation ( 2 ) , ubiquitination ( 2 )
Induce interaction with:
14-3-3 theta (mouse) ( 4 , 5 ) , 14-3-3 zeta (mouse) ( 5 )
Inhibit interaction with:
ZO1 (rat) ( 1 )

References 

1

Thévenin AF, et al. (2017) Phosphorylation regulates connexin43/ZO-1 binding and release, an important step in gap junction turnover. Mol Biol Cell 28, 3595-3608
29021339   Curated Info

2

Li X, et al. (2015) Inhibition of connexin43 improves functional recovery after ischemic brain injury in neonatal rats. Glia 63, 1553-67
25988944   Curated Info

3

Huang RY, et al. (2011) Identification of CaMKII phosphorylation sites in Connexin43 by high-resolution mass spectrometry. J Proteome Res 10, 1098-109
21158428   Curated Info

4

Park DJ, et al. (2007) Akt phosphorylates Connexin43 on Ser373, a "mode-1" binding site for 14-3-3. Cell Commun Adhes 14, 211-26
18163231   Curated Info

5

Park DJ, et al. (2006) Molecular dynamics and in vitro analysis of Connexin43: A new 14-3-3 mode-1 interacting protein. Protein Sci 15, 2344-55
17008717   Curated Info

6

Yogo K, et al. (2006) PKA implicated in the phosphorylation of Cx43 induced by stimulation with FSH in rat granulosa cells. J Reprod Dev 52, 321-8
16474210   Curated Info

7

Axelsen LN, et al. (2006) Identification of ischemia-regulated phosphorylation sites in connexin43: A possible target for the antiarrhythmic peptide analogue rotigaptide (ZP123). J Mol Cell Cardiol 40, 790-8
16678851   Curated Info

8

Yogo K, et al. (2002) Identification and functional analysis of novel phosphorylation sites in Cx43 in rat primary granulosa cells. FEBS Lett 531, 132-6
12417300   Curated Info