Ser373
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Home > Phosphorylation Site Page: > Ser373  -  GJA1 (rat)

Site Information
RAssRAssRPRPDDL   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 459209

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 10 ) , immunoassay ( 2 ) , immunoprecipitation ( 3 ) , mass spectrometry ( 9 ) , mass spectrometry (in vitro) ( 5 ) , mutation of modification site ( 3 , 8 , 10 ) , phospho-antibody ( 1 , 2 , 4 , 6 ) , phosphoamino acid analysis ( 10 ) , phosphopeptide mapping ( 10 ) , western blotting ( 1 , 2 , 3 , 4 , 6 )
Relevant cell line - cell type - tissue:
astrocyte ( 2 ) , astrocyte-'brain, cerebrum' ( 4 ) , granulosa ( 10 ) , heart ( 9 ) , HeLa (cervical) ( 3 , 10 ) , MDCK (epithelial) ( 3 ) , preovulatory follicle granulosa ( 8 ) , Rat1 (fibroblast) ( 6 ) , spinal cord ( 1 )

Upstream Regulation
Regulatory protein:
DRD2 (human) ( 1 ) , THY1 (rat) ( 2 )
Putative in vivo kinases:
Akt1 (rat) ( 2 ) , Akt3 (rat) ( 6 ) , PKACA (human) ( 8 )
Kinases, in vitro:
Akt1 (human) ( 6 , 7 ) , CAMK2A (human) ( 5 ) , PKACA (human) ( 8 ) , PKCA (human) ( 9 ) , PKCE (human) ( 9 )
Treatments:
Akt_inhibitor_VIII ( 2 ) , cAMP_analog ( 1 ) , EGF ( 6 ) , H-89 ( 1 , 8 ) , LY294002 ( 2 ) , PD98059 ( 8 ) , peptide ( 1 ) , TNF ( 2 ) , urofollitropin ( 8 , 10 )

Downstream Regulation
Effects of modification on GJA1:
activity, induced ( 10 ) , intracellular localization ( 3 ) , molecular association, regulation ( 3 , 6 , 7 ) , protein degradation ( 4 ) , ubiquitination ( 4 )
Induce interaction with:
14-3-3 theta (mouse) ( 6 , 7 ) , 14-3-3 zeta (mouse) ( 7 )
Inhibit interaction with:
ZO1 (rat) ( 3 )

References 

1

Yang Q, et al. (2023) Mirror image pain mediated by D2 receptor regulation of astrocytic Cx43 phosphorylation and channel opening. Biochim Biophys Acta Mol Basis Dis 1869, 166657
36716897   Curated Info

2

Pérez-Núñez R, et al. (2023) Protein kinase B (AKT) upregulation and Thy-1-αβ integrin-induced phosphorylation of Connexin43 by activated AKT in astrogliosis. J Neuroinflammation 20, 5
36609298   Curated Info

3

Thévenin AF, et al. (2017) Phosphorylation regulates connexin43/ZO-1 binding and release, an important step in gap junction turnover. Mol Biol Cell 28, 3595-3608
29021339   Curated Info

4

Li X, et al. (2015) Inhibition of connexin43 improves functional recovery after ischemic brain injury in neonatal rats. Glia 63, 1553-67
25988944   Curated Info

5

Huang RY, et al. (2011) Identification of CaMKII phosphorylation sites in Connexin43 by high-resolution mass spectrometry. J Proteome Res 10, 1098-109
21158428   Curated Info

6

Park DJ, et al. (2007) Akt phosphorylates Connexin43 on Ser373, a "mode-1" binding site for 14-3-3. Cell Commun Adhes 14, 211-26
18163231   Curated Info

7

Park DJ, et al. (2006) Molecular dynamics and in vitro analysis of Connexin43: A new 14-3-3 mode-1 interacting protein. Protein Sci 15, 2344-55
17008717   Curated Info

8

Yogo K, et al. (2006) PKA implicated in the phosphorylation of Cx43 induced by stimulation with FSH in rat granulosa cells. J Reprod Dev 52, 321-8
16474210   Curated Info

9

Axelsen LN, et al. (2006) Identification of ischemia-regulated phosphorylation sites in connexin43: A possible target for the antiarrhythmic peptide analogue rotigaptide (ZP123). J Mol Cell Cardiol 40, 790-8
16678851   Curated Info

10

Yogo K, et al. (2002) Identification and functional analysis of novel phosphorylation sites in Cx43 in rat primary granulosa cells. FEBS Lett 531, 132-6
12417300   Curated Info