Ser369

Site Information
RPssRAssRAssRPR SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 459208

In vivo Characterization
Methods used to characterize site in vivo:	electrophoretic mobility shift ( 4 ) , immunoprecipitation ( 1 , 3 ) , mass spectrometry ( 1 ) , mutation of modification site ( 1 , 3 , 4 ) , phospho-antibody ( 2 ) , western blotting ( 1 , 2 )
Relevant cell line - cell type - tissue:	293 (epithelial) ( 1 ) , IAR20 (epithelial) ( 1 ) , MLO-Y4 (osteoblast) ( 3 ) , stromal ( 2 ) , trophoblast ( 1 )

Downstream Regulation
Effects of modification on GJA1:	intracellular localization ( 1 ) , protein conformation ( 4 )

References
1	Dukic AR, et al. (2017) Ezrin-anchored PKA phosphorylates serine 369 and 373 on connexin 43 to enhance gap junction assembly, communication, and cell fusion. Biochem J 29259079 Curated Info
2	Balla P, et al. (2015) Prognostic impact of reduced connexin43 expression and gap junction coupling of neoplastic stromal cells in giant cell tumor of bone. PLoS One 10, e0125316 25933380 Curated Info
3	Batra N, et al. (2014) Direct regulation of osteocytic connexin 43 hemichannels through AKT kinase activated by mechanical stimulation. J Biol Chem 289, 10582-91 24563481 Curated Info
4	Grosely R, et al. (2013) Effects of Phosphorylation on the Structure and Backbone Dynamics of the Intrinsically Disordered Connexin43 C-terminal Domain. J Biol Chem 288, 24857-70 23828237 Curated Info