Ser369
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Home > Phosphorylation Site Page: > Ser369  -  GJA1 (rat)

Site Information
RPssRAssRAssRPR   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 459208

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 11 ) , electrophoretic mobility shift ( 5 ) , immunoprecipitation ( 10 ) , mass spectrometry ( 7 ) , mass spectrometry (in vitro) ( 2 ) , microscopy-colocalization with upstream kinase ( 5 , 10 ) , mutation of modification site ( 6 , 11 ) , phospho-antibody ( 3 , 5 , 8 , 10 ) , phosphoamino acid analysis ( 11 ) , phosphopeptide mapping ( 11 ) , western blotting ( 5 , 8 , 10 )
Relevant cell line - cell type - tissue:
CHO (fibroblast) ( 10 ) , granulosa ( 11 ) , heart ( 7 ) , HeLa (cervical) ( 10 , 11 ) , myometrial ( 8 ) , NRK (fibroblast) ( 10 ) , NRK-E51 (renal) ( 3 , 5 ) , preovulatory follicle granulosa ( 6 ) , RIN (epithelial) ( 5 )

Upstream Regulation
Putative in vivo kinases:
PKACA (human) ( 6 ) , PKCA (rat) ( 5 , 10 )
Kinases, in vitro:
Akt1 (human) ( 4 ) , CAMK2A (human) ( 2 ) , PKACA (human) ( 6 ) , PKCA (human) ( 3 , 7 ) , PKCA (rat) ( 9 ) , PKCE (human) ( 7 )
Treatments:
2,2'-DCB ( 8 ) , H-89 ( 6 ) , ischemia ( 5 ) , PD98059 ( 6 ) , phorbol_ester ( 3 , 10 ) , urofollitropin ( 6 , 11 )

Downstream Regulation
Effects of modification on GJA1:
activity, induced ( 11 ) , intracellular localization ( 5 , 10 ) , protein conformation ( 5 )

References 

1

Bertholomey ML, et al. (2018) Phosphoproteomic Analysis of the Amygdala Response to Adolescent Glucocorticoid Exposure Reveals G-Protein Coupled Receptor Kinase 2 as a Target for Reducing Motivation for Alcohol. Proteomes 6
30322021   Curated Info

2

Huang RY, et al. (2011) Identification of CaMKII phosphorylation sites in Connexin43 by high-resolution mass spectrometry. J Proteome Res 10, 1098-109
21158428   Curated Info

3

Solan JL, et al. (2007) Phosphorylation at S365 is a gatekeeper event that changes the structure of Cx43 and prevents down-regulation by PKC. J Cell Biol 179, 1301-9
18086922   Curated Info

4

Park DJ, et al. (2007) Akt phosphorylates Connexin43 on Ser373, a "mode-1" binding site for 14-3-3. Cell Commun Adhes 14, 211-26
18163231   Curated Info

5

Ek-Vitorin JF, et al. (2006) Selectivity of connexin 43 channels is regulated through protein kinase C-dependent phosphorylation. Circ Res 98, 1498-505
16709897   Curated Info

6

Yogo K, et al. (2006) PKA implicated in the phosphorylation of Cx43 induced by stimulation with FSH in rat granulosa cells. J Reprod Dev 52, 321-8
16474210   Curated Info

7

Axelsen LN, et al. (2006) Identification of ischemia-regulated phosphorylation sites in connexin43: A possible target for the antiarrhythmic peptide analogue rotigaptide (ZP123). J Mol Cell Cardiol 40, 790-8
16678851   Curated Info

8

Chung D, Loch Caruso R (2005) 2,2'-Dichlorobiphenyl decreases amplitude and synchronization of uterine contractions through MAPK1-mediated phosphorylation of GJA1 (connexin43) and inhibition of myometrial gap junctions. Biol Reprod 73, 974-82
16000550   Curated Info

9

Bao X, Reuss L, Altenberg GA (2004) Regulation of purified and reconstituted connexin 43 hemichannels by protein kinase C-mediated phosphorylation of Serine 368. J Biol Chem 279, 20058-66
14973142   Curated Info

10

Solan JL, Fry MD, TenBroek EM, Lampe PD (2003) Connexin43 phosphorylation at S368 is acute during S and G2/M and in response to protein kinase C activation. J Cell Sci 116, 2203-11
12697837   Curated Info

11

Yogo K, et al. (2002) Identification and functional analysis of novel phosphorylation sites in Cx43 in rat primary granulosa cells. FEBS Lett 531, 132-6
12417300   Curated Info