Ser369

Site Information
RPssRAssRAssRPR SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 459208

In vivo Characterization
Methods used to characterize site in vivo:	[32P] bio-synthetic labeling ( 11 ) , electrophoretic mobility shift ( 5 ) , immunoprecipitation ( 10 ) , mass spectrometry ( 1 , 7 ) , mass spectrometry (in vitro) ( 2 ) , microscopy-colocalization with upstream kinase ( 5 , 10 ) , mutation of modification site ( 6 , 11 ) , phospho-antibody ( 3 , 5 , 8 , 10 ) , phosphoamino acid analysis ( 11 ) , phosphopeptide mapping ( 11 ) , western blotting ( 5 , 8 , 10 )
Relevant cell line - cell type - tissue:	brain ( 1 ) , CHO (fibroblast) ( 10 ) , granulosa ( 11 ) , heart ( 7 ) , HeLa (cervical) ( 10 , 11 ) , myometrial ( 8 ) , NRK (fibroblast) ( 10 ) , NRK-E51 (renal) ( 3 , 5 ) , preovulatory follicle granulosa ( 6 ) , RIN (epithelial) ( 5 )

In vivo Characterization

Methods used to characterize site in vivo:

[32P] bio-synthetic labeling ( 11 ) , electrophoretic mobility shift ( 5 ) , immunoprecipitation ( 10 ) , mass spectrometry ( 1 , 7 ) , mass spectrometry (in vitro) ( 2 ) , microscopy-colocalization with upstream kinase ( 5 , 10 ) , mutation of modification site ( 6 , 11 ) , phospho-antibody ( 3 , 5 , 8 , 10 ) , phosphoamino acid analysis ( 11 ) , phosphopeptide mapping ( 11 ) , western blotting ( 5 , 8 , 10 )

Relevant cell line - cell type - tissue:

brain ( 1 ) , CHO (fibroblast) ( 10 ) , granulosa ( 11 ) , heart ( 7 ) , HeLa (cervical) ( 10 , 11 ) , myometrial ( 8 ) , NRK (fibroblast) ( 10 ) , NRK-E51 (renal) ( 3 , 5 ) , preovulatory follicle granulosa ( 6 ) , RIN (epithelial) ( 5 )

Upstream Regulation
Putative in vivo kinases:	PKACA (human) ( 6 ) , PKCA (rat) ( 5 , 10 )
Kinases, in vitro:	Akt1 (human) ( 4 ) , CAMK2A (human) ( 2 ) , PKACA (human) ( 6 ) , PKCA (human) ( 3 , 7 ) , PKCA (rat) ( 9 ) , PKCE (human) ( 7 )
Treatments:	2,2'-DCB ( 8 ) , corticosterone ( 1 ) , ethanol ( 1 ) , H-89 ( 6 ) , ischemia ( 5 ) , PD98059 ( 6 ) , phorbol_ester ( 3 , 10 ) , sucrose ( 1 ) , urofollitropin ( 6 , 11 )

Downstream Regulation
Effects of modification on GJA1:	activity, induced ( 11 ) , intracellular localization ( 5 , 10 ) , protein conformation ( 5 )

References
1	Bertholomey ML, et al. (2018) Phosphoproteomic Analysis of the Amygdala Response to Adolescent Glucocorticoid Exposure Reveals G-Protein Coupled Receptor Kinase 2 as a Target for Reducing Motivation for Alcohol. Proteomes 6 30322021 Curated Info
2	Huang RY, et al. (2011) Identification of CaMKII phosphorylation sites in Connexin43 by high-resolution mass spectrometry. J Proteome Res 10, 1098-109 21158428 Curated Info
3	Solan JL, et al. (2007) Phosphorylation at S365 is a gatekeeper event that changes the structure of Cx43 and prevents down-regulation by PKC. J Cell Biol 179, 1301-9 18086922 Curated Info
4	Park DJ, et al. (2007) Akt phosphorylates Connexin43 on Ser373, a "mode-1" binding site for 14-3-3. Cell Commun Adhes 14, 211-26 18163231 Curated Info
5	Ek-Vitorin JF, et al. (2006) Selectivity of connexin 43 channels is regulated through protein kinase C-dependent phosphorylation. Circ Res 98, 1498-505 16709897 Curated Info
6	Yogo K, et al. (2006) PKA implicated in the phosphorylation of Cx43 induced by stimulation with FSH in rat granulosa cells. J Reprod Dev 52, 321-8 16474210 Curated Info
7	Axelsen LN, et al. (2006) Identification of ischemia-regulated phosphorylation sites in connexin43: A possible target for the antiarrhythmic peptide analogue rotigaptide (ZP123). J Mol Cell Cardiol 40, 790-8 16678851 Curated Info
8	Chung D, Loch Caruso R (2005) 2,2'-Dichlorobiphenyl decreases amplitude and synchronization of uterine contractions through MAPK1-mediated phosphorylation of GJA1 (connexin43) and inhibition of myometrial gap junctions. Biol Reprod 73, 974-82 16000550 Curated Info
9	Bao X, Reuss L, Altenberg GA (2004) Regulation of purified and reconstituted connexin 43 hemichannels by protein kinase C-mediated phosphorylation of Serine 368. J Biol Chem 279, 20058-66 14973142 Curated Info
10	Solan JL, Fry MD, TenBroek EM, Lampe PD (2003) Connexin43 phosphorylation at S368 is acute during S and G2/M and in response to protein kinase C activation. J Cell Sci 116, 2203-11 12697837 Curated Info
11	Yogo K, et al. (2002) Identification and functional analysis of novel phosphorylation sites in Cx43 in rat primary granulosa cells. FEBS Lett 531, 132-6 12417300 Curated Info