Ser365
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage PhosphoSitePlus®
Powered by Cell Signaling Technology
Home > Phosphorylation Site Page: > Ser365  -  GJA1 (rat)

Site Information
IVDQRPssRAssRAs   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 459207

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 9 ) , immunoprecipitation ( 2 ) , mass spectrometry ( 4 , 6 , 8 ) , mass spectrometry (in vitro) ( 5 ) , mutation of modification site ( 2 , 6 , 7 , 9 ) , phospho-antibody ( 6 ) , phosphoamino acid analysis ( 9 ) , phosphopeptide mapping ( 9 ) , western blotting ( 2 )
Relevant cell line - cell type - tissue:
'brain, brainstem' ( 4 ) , 'brain, cerebellum' ( 4 ) , 'brain, cerebral cortex' ( 4 ) , granulosa ( 9 ) , heart ( 4 , 8 ) , HeLa (cervical) ( 2 , 9 ) , intestine ( 4 ) , MDCK (epithelial) ( 2 , 6 ) , NRK-E51 (renal) ( 6 ) , preovulatory follicle granulosa ( 7 ) , spleen ( 4 ) , stomach ( 4 ) , testis ( 4 )

Upstream Regulation
Putative in vivo kinases:
PKACA (human) ( 7 )
Kinases, in vitro:
CAMK2A (human) ( 5 ) , PKACA (human) ( 7 ) , PKCA (human) ( 8 ) , PKCE (human) ( 8 )
Treatments:
H-89 ( 7 ) , PD98059 ( 7 ) , urofollitropin ( 7 , 9 )

Downstream Regulation
Effects of modification on GJA1:
activity, induced ( 2 , 9 ) , protein conformation ( 6 )

References 

1

Bertholomey ML, et al. (2018) Phosphoproteomic Analysis of the Amygdala Response to Adolescent Glucocorticoid Exposure Reveals G-Protein Coupled Receptor Kinase 2 as a Target for Reducing Motivation for Alcohol. Proteomes 6
30322021   Curated Info

2

Thévenin AF, et al. (2017) Phosphorylation regulates connexin43/ZO-1 binding and release, an important step in gap junction turnover. Mol Biol Cell 28, 3595-3608
29021339   Curated Info

3

Sakurai T, Tsuchida M, Lampe PD, Murakami M (2013) Cardiomyocyte FGF signaling is required for Cx43 phosphorylation and cardiac gap junction maintenance. Exp Cell Res 319, 2152-65
23742896   Curated Info

4

Lundby A, et al. (2012) Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues. Nat Commun 3, 876
22673903   Curated Info

5

Huang RY, et al. (2011) Identification of CaMKII phosphorylation sites in Connexin43 by high-resolution mass spectrometry. J Proteome Res 10, 1098-109
21158428   Curated Info

6

Solan JL, et al. (2007) Phosphorylation at S365 is a gatekeeper event that changes the structure of Cx43 and prevents down-regulation by PKC. J Cell Biol 179, 1301-9
18086922   Curated Info

7

Yogo K, et al. (2006) PKA implicated in the phosphorylation of Cx43 induced by stimulation with FSH in rat granulosa cells. J Reprod Dev 52, 321-8
16474210   Curated Info

8

Axelsen LN, et al. (2006) Identification of ischemia-regulated phosphorylation sites in connexin43: A possible target for the antiarrhythmic peptide analogue rotigaptide (ZP123). J Mol Cell Cardiol 40, 790-8
16678851   Curated Info

9

Yogo K, et al. (2002) Identification and functional analysis of novel phosphorylation sites in Cx43 in rat primary granulosa cells. FEBS Lett 531, 132-6
12417300   Curated Info