Ser21
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Site Information
sGRARtssFAEPGGG   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448584

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 42 ) , mass spectrometry ( 1 , 3 , 5 , 6 , 7 , 11 , 17 , 18 , 19 , 21 , 22 , 23 , 24 , 25 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 46 , 48 , 49 , 50 , 54 , 55 ) , microscopy-colocalization with upstream kinase ( 45 ) , mutation of modification site ( 15 , 43 , 69 ) , phospho-antibody ( 4 , 8 , 10 , 12 , 15 , 16 , 20 , 26 , 41 , 42 , 45 , 47 , 51 , 52 , 53 , 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 , 64 , 65 , 66 , 67 , 68 , 69 , 70 , 72 , 73 ) , western blotting ( 4 , 8 , 10 , 12 , 15 , 16 , 20 , 26 , 41 , 42 , 45 , 47 , 51 , 52 , 53 , 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 , 64 , 65 , 66 , 67 , 68 , 69 , 70 , 72 , 73 )
Disease tissue studied:
breast cancer ( 57 ) , neuroblastoma ( 11 , 26 )
Relevant cell line - cell type - tissue:
'3T3-L1, differentiated' (adipocyte) ( 3 ) , 'brain, cerebral cortex' ( 62 , 66 , 70 ) , 'brain, embryonic' ( 49 , 54 ) , 'brain, hippocampus' ( 66 ) , 'brain, striatum' ( 66 ) , 'muscle, skeletal' ( 47 , 56 , 59 , 61 , 69 ) , 'muscle, skeletal' [RICTOR (mouse), homozygous knockout] ( 51 ) , 'neuron, cortical' ( 8 ) , 'neuron, hippocampal' ( 8 ) , 'stem, embryonic' ( 21 , 22 , 23 , 24 , 47 , 69 ) , 293 (epithelial) ( 16 ) , 293T (epithelial) ( 73 ) , 3T3 (fibroblast) ( 42 , 52 , 57 ) , 3T3 (fibroblast) [INSR (mouse), transfection] ( 42 ) , 3T3-L1 (fibroblast) ( 36 , 37 , 38 , 46 ) , adipose tissue ( 47 , 59 ) , BaF3 ('B lymphocyte, precursor') ( 42 ) , brain ( 4 , 7 , 55 ) , COS (fibroblast) ( 42 ) , dendritic cell ( 52 ) , dendritic cell [MAPKAPK2 (mouse), homozygous knockout] ( 52 ) , dendritic cell [MSK1 (mouse), homozygous knockout] ( 52 ) , embryo ( 7 ) , epithelial ( 45 ) , fibroblast-lung ( 17 , 18 , 19 , 39 , 40 ) , GD25 (fibroblast) ( 72 ) , heart ( 15 , 47 , 50 , 59 ) , hepatocyte-liver [TSC1 (mouse), homozygous knockout] ( 10 ) , keratinocyte ( 67 ) , liver ( 6 , 7 , 20 , 25 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 47 , 48 , 59 , 68 , 69 ) , liver [leptin (mouse), homozygous knockout] ( 6 ) , macrophage-peritoneum ( 5 ) , MCF-7 (breast cell) ( 57 ) , MEF (fibroblast) ( 12 , 16 , 63 , 64 ) , MEF (fibroblast) [Sin1 (mouse), homozygous knockout] ( 41 ) , myocyte-heart ( 43 , 58 ) , N1E-115 (neuron) ( 11 ) , Neuro-2a (neuron) ( 26 ) , neuron-'brain, hippocampus' ( 65 ) , parotid acinar-salivary gland ( 60 ) , parotid acinar-salivary gland [Akt1 (mouse)] ( 60 ) , prostate ( 45 ) , RAW 264.7 (macrophage) ( 1 ) , Swiss 3T3 (fibroblast) ( 73 )

Upstream Regulation
Regulatory protein:
Akt1 (human) ( 45 ) , Akt1 (mouse) ( 60 ) , Akt2 (mouse) ( 61 ) , GRK2 (mouse) ( 53 ) , ITGB1 (human) ( 72 ) , PDK1 (mouse) ( 47 ) , RICTOR (human) ( 16 ) , RICTOR (mouse) ( 16 ) , RPS23 (mouse) ( 26 ) , Sin1 (mouse) ( 64 ) , TSC1 (mouse) ( 10 , 63 ) , TSC2 (mouse) ( 63 )
Putative in vivo kinases:
PKCA (human) ( 73 ) , PKCB (human) ( 73 ) , PKCH (human) ( 73 )
Treatments:
8-Rp-cAMP ( 57 ) , A-443654 ( 42 ) , Akt-I-1 ( 42 ) , Akt_inhibitor_VIII ( 42 ) , antichymotrypsin ( 62 ) , BI-D1870 ( 52 ) , blood pressure ( 43 ) , culturing_of_cells ( 8 ) , fasting ( 20 ) , FGF1 ( 44 ) , GF109203X ( 67 , 73 ) , GSK-3_inhibitor_X ( 15 ) , high-fat diet ( 59 ) , IGF-1 ( 63 , 67 , 73 ) , IL-3 ( 42 ) , insulin ( 3 , 42 , 47 , 51 , 53 , 56 , 59 , 64 , 73 ) , isoproterenol ( 15 ) , L803-mts ( 68 ) , lithium ( 26 ) , LPA ( 73 ) , LPS ( 52 ) , LY294002 ( 3 , 42 , 73 ) , memantine ( 66 ) , MK-2206 ( 3 ) , MK-591 ( 4 ) , muscle contraction ( 61 ) , PDGF ( 73 ) , phorbol_ester ( 67 , 73 ) , physostigmine ( 66 ) , pilocarpine ( 66 ) , pregnancy ( 58 ) , rapamycin ( 42 , 63 ) , refeeding ( 20 ) , Ro31-8220 ( 73 ) , serum ( 16 , 64 ) , serum_starvation ( 64 ) , siRNA ( 53 ) , SL0101 ( 52 ) , U0126 ( 42 ) , wortmannin ( 63 , 73 )

Downstream Regulation
Effects of modification on GSK3A:
enzymatic activity, inhibited ( 43 , 69 ) , protein degradation ( 70 )
Effects of modification on biological processes:
apoptosis, induced ( 43 ) , cell growth, altered ( 43 ) , cytoskeletal reorganization ( 43 ) , transcription, altered ( 43 )

Disease / Diagnostics Relevance
Relevant diseases:
Alzheimer's disease ( 70 ) , type 2 diabetes ( 59 , 70 )

References 

1

Pinto SM, et al. (2015) Quantitative phosphoproteomic analysis of IL-33-mediated signaling. Proteomics 15, 532-44
25367039   Curated Info

2

Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704
24719451   Curated Info

3

Humphrey SJ, et al. (2013) Dynamic Adipocyte Phosphoproteome Reveals that Akt Directly Regulates mTORC2. Cell Metab 17, 1009-20
23684622   Curated Info

4

Chu J, Lauretti E, Di Meco A, Praticò D (2013) FLAP pharmacological blockade modulates metabolism of endogenous tau in vivo . Transl Psychiatry 3, e333
24301651   Curated Info

5

Wu X, et al. (2012) Investigation of receptor interacting protein (RIP3)-dependent protein phosphorylation by quantitative phosphoproteomics. Mol Cell Proteomics 11, 1640-51
22942356   Curated Info

6

Grimsrud PA, et al. (2012) A quantitative map of the liver mitochondrial phosphoproteome reveals posttranslational control of ketogenesis. Cell Metab 16, 672-83
23140645   Curated Info

7

Stokes MP, et al. (2012) PTMScan Direct: Identification and Quantification of Peptides from Critical Signaling Proteins by Immunoaffinity Enrichment Coupled with LC-MS/MS. Mol Cell Proteomics 11, 187-201
22322096   Curated Info

8

Diez H, Garrido JJ, Wandosell F (2012) Specific roles of Akt iso forms in apoptosis and axon growth regulation in neurons. PLoS One 7, e32715
22509246   Curated Info

9

Tang W, et al. (2011) A PLCβ/PI3Kγ-GSK3 signaling pathway regulates cofilin phosphatase slingshot2 and neutrophil polarization and chemotaxis. Dev Cell 21, 1038-50
22172670   Curated Info

10

Yecies JL, et al. (2011) Akt Stimulates Hepatic SREBP1c and Lipogenesis through Parallel mTORC1-Dependent and Independent Pathways. Cell Metab 14, 21-32
21723501   Curated Info

11

Wang Y, et al. (2011) Spatial phosphoprotein profiling reveals a compartmentalized extracellular signal-regulated kinase switch governing neurite growth and retraction. J Biol Chem 286, 18190-201
21454597   Curated Info

12

Xie X, et al. (2011) IkappaB kinase epsilon and TANK-binding kinase 1 activate AKT by direct phosphorylation. Proc Natl Acad Sci U S A 108, 6474-9
21464307   Curated Info

13

Rodgers JT, Vogel RO, Puigserver P (2011) Clk2 and B56β Mediate Insulin-Regulated Assembly of the PP2A Phosphatase Holoenzyme Complex on Akt. Mol Cell 41, 471-9
21329884   Curated Info

14

Chen S, Wasserman DH, MacKintosh C, Sakamoto K (2011) Mice with AS160/TBC1D4-Thr649Ala knockin mutation are glucose intolerant with reduced insulin sensitivity and altered GLUT4 trafficking. Cell Metab 13, 68-79
21195350   Curated Info

15

Webb IG, et al. (2010) Constitutive glycogen synthase kinase-3alpha/beta activity protects against chronic beta-adrenergic remodelling of the heart. Cardiovasc Res 87, 494-503
20299330   Curated Info

16

Treins C, et al. (2010) Rictor is a novel target of p70 S6 kinase-1. Oncogene 29, 1003-16
19935711   Curated Info

17

Guo A (2010) CST Curation Set: 9034; Year: 2010; Biosample/Treatment: cell line, mouse lung fibroblasts/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-Akt Substrate (RXRXXS/T) (110B7) Rabbit mAb Cat#: 9614, PTMScan(R) Phospho-Akt Substrate Motif (RXXS*/T*) Immunoaffinity Beads Cat#: 1978
Curated Info

18

Guo A (2010) CST Curation Set: 9033; Year: 2010; Biosample/Treatment: cell line, mouse lung fibroblasts/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-Akt Substrate (RXRXXS/T) (110B7) Rabbit mAb Cat#: 9614, PTMScan(R) Phospho-Akt Substrate Motif (RXXS*/T*) Immunoaffinity Beads Cat#: 1978
Curated Info

19

Guo A (2010) CST Curation Set: 9029; Year: 2010; Biosample/Treatment: cell line, mouse lung fibroblasts/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-Akt Substrate (RXRXXS/T) (110B7) Rabbit mAb Cat#: 9614, PTMScan(R) Phospho-Akt Substrate Motif (RXXS*/T*) Immunoaffinity Beads Cat#: 1978
Curated Info

20

Rodgers JT, Haas W, Gygi SP, Puigserver P (2010) Cdc2-like kinase 2 is an insulin-regulated suppressor of hepatic gluconeogenesis. Cell Metab 11, 23-34
20074525   Curated Info

21

Tucker M (2009) CST Curation Set: 8561; Year: 2009; Biosample/Treatment: cell line, embryonic stem cells (mouse)(PTEN)/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-Akt Substrate (RXRXXS/T) (110B7) Rabbit mAb Cat#: 9614, PTMScan(R) Phospho-Akt Substrate Motif (RXXS*/T*) Immunoaffinity Beads Cat#: 1978
Curated Info

22

Tucker M (2009) CST Curation Set: 8560; Year: 2009; Biosample/Treatment: cell line, embryonic stem cells (mouse)(PTEN)/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST]
Curated Info

23

Tucker M (2009) CST Curation Set: 8555; Year: 2009; Biosample/Treatment: cell line, embryonic stem cells (mouse)(PTEN)/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-Akt Substrate (RXRXXS/T) (110B7) Rabbit mAb Cat#: 9614, PTMScan(R) Phospho-Akt Substrate Motif (RXXS*/T*) Immunoaffinity Beads Cat#: 1978
Curated Info

24

Tucker M (2009) CST Curation Set: 8557; Year: 2009; Biosample/Treatment: cell line, embryonic stem cells (mouse)(PTEN)/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-Akt Substrate (RXRXXS/T) (110B7) Rabbit mAb Cat#: 9614, PTMScan(R) Phospho-Akt Substrate Motif (RXXS*/T*) Immunoaffinity Beads Cat#: 1978
Curated Info

25

Guo A (2009) CST Curation Set: 8487; Year: 2009; Biosample/Treatment: tissue, liver(STK11)/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST]
Curated Info

26

Zhang YW, et al. (2009) A functional mouse retroposed gene Rps23r1 reduces Alzheimer's beta-amyloid levels and tau phosphorylation. Neuron 64, 328-40
19914182   Curated Info

27

Guo A (2009) CST Curation Set: 7827; Year: 2009; Biosample/Treatment: tissue, liver(STK11)/Metformin; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-Akt Substrate (RXRXXS/T) (110B7) Rabbit mAb Cat#: 9614, PTMScan(R) Phospho-Akt Substrate Motif (RXXS*/T*) Immunoaffinity Beads Cat#: 1978
Curated Info

28

Guo A (2009) CST Curation Set: 7822; Year: 2009; Biosample/Treatment: tissue, liver/Metformin; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-Akt Substrate (RXRXXS/T) (110B7) Rabbit mAb Cat#: 9614, PTMScan(R) Phospho-Akt Substrate Motif (RXXS*/T*) Immunoaffinity Beads Cat#: 1978
Curated Info

29

Guo A (2009) CST Curation Set: 7825; Year: 2009; Biosample/Treatment: tissue, liver(STK11)/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-Akt Substrate (RXRXXS/T) (110B7) Rabbit mAb Cat#: 9614, PTMScan(R) Phospho-Akt Substrate Motif (RXXS*/T*) Immunoaffinity Beads Cat#: 1978
Curated Info

30

Guo A (2009) CST Curation Set: 7826; Year: 2009; Biosample/Treatment: tissue, liver(STK11)/Metformin; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-Akt Substrate (RXRXXS/T) (110B7) Rabbit mAb Cat#: 9614, PTMScan(R) Phospho-Akt Substrate Motif (RXXS*/T*) Immunoaffinity Beads Cat#: 1978
Curated Info

31

Guo A (2009) CST Curation Set: 7791; Year: 2009; Biosample/Treatment: tissue, liver(STK11)/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-(Ser/Thr) PKD Substrate Antibody Cat#: 4381, PTMScan(R) Phospho-PKD Substrate Motif (LXRXXpS/pT) Immunoaffinity Beads Cat#: 1986
Curated Info

32

Guo A (2009) CST Curation Set: 7802; Year: 2009; Biosample/Treatment: tissue, liver(STK11)/Metformin; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-(Ser/Thr) PKD Substrate Antibody Cat#: 4381, PTMScan(R) Phospho-PKD Substrate Motif (LXRXXpS/pT) Immunoaffinity Beads Cat#: 1986
Curated Info

33

Guo A (2009) CST Curation Set: 7821; Year: 2009; Biosample/Treatment: tissue, liver/Metformin; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-Akt Substrate (RXRXXS/T) (110B7) Rabbit mAb Cat#: 9614, PTMScan(R) Phospho-Akt Substrate Motif (RXXS*/T*) Immunoaffinity Beads Cat#: 1978
Curated Info

34

Guo A (2009) CST Curation Set: 7819; Year: 2009; Biosample/Treatment: tissue, liver/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-Akt Substrate (RXRXXS/T) (110B7) Rabbit mAb Cat#: 9614, PTMScan(R) Phospho-Akt Substrate Motif (RXXS*/T*) Immunoaffinity Beads Cat#: 1978
Curated Info

35

Guo A (2009) CST Curation Set: 7820; Year: 2009; Biosample/Treatment: tissue, liver/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-Akt Substrate (RXRXXS/T) (110B7) Rabbit mAb Cat#: 9614, PTMScan(R) Phospho-Akt Substrate Motif (RXXS*/T*) Immunoaffinity Beads Cat#: 1978
Curated Info

36

Guo A (2009) CST Curation Set: 7449; Year: 2009; Biosample/Treatment: cell line, 3T3-L1/Insulin; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-Akt Substrate (RXRXXS/T) (110B7) Rabbit mAb Cat#: 9614, PTMScan(R) Phospho-Akt Substrate Motif (RXXS*/T*) Immunoaffinity Beads Cat#: 1978
Curated Info

37

Guo A (2009) CST Curation Set: 7451; Year: 2009; Biosample/Treatment: cell line, 3T3-L1/Insulin; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-Akt Substrate (RXRXXS*/T*) (23C8D2) Rabbit mAb Cat#: 10001, PTMScan(R) Phospho-Akt Substrate Motif (RXRXXS*/T*) Immunoaffinity Beads Cat#: 1979
Curated Info

38

Guo A (2009) CST Curation Set: 7448; Year: 2009; Biosample/Treatment: cell line, 3T3-L1/control; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-Akt Substrate (RXRXXS/T) (110B7) Rabbit mAb Cat#: 9614, PTMScan(R) Phospho-Akt Substrate Motif (RXXS*/T*) Immunoaffinity Beads Cat#: 1978
Curated Info

39

Guo A (2009) CST Curation Set: 6843; Year: 2009; Biosample/Treatment: cell line, mouse lung fibroblasts/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-Akt Substrate (RXRXXS*/T*) (23C8D2) Rabbit mAb Cat#: 10001, PTMScan(R) Phospho-Akt Substrate Motif (RXRXXS*/T*) Immunoaffinity Beads Cat#: 1979
Curated Info

40

Guo A (2009) CST Curation Set: 6842; Year: 2009; Biosample/Treatment: cell line, mouse lung fibroblasts/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-Akt Substrate (RXRXXS*/T*) (23C8D2) Rabbit mAb Cat#: 10001, PTMScan(R) Phospho-Akt Substrate Motif (RXRXXS*/T*) Immunoaffinity Beads Cat#: 1979
Curated Info

41

Feldman ME, et al. (2009) Active-site inhibitors of mTOR target rapamycin-resistant outputs of mTORC1 and mTORC2. PLoS Biol 7, e38
19209957   Curated Info

42

van Gorp AG, et al. (2009) AGC kinases regulate phosphorylation and activation of eukaryotic translation initiation factor 4B. Oncogene 28, 95-106
18836482   Curated Info

43

Matsuda T, et al. (2008) Distinct roles of GSK-3alpha and GSK-3beta phosphorylation in the heart under pressure overload. Proc Natl Acad Sci U S A 105, 20900-5
19106302   Curated Info

44

Partovian C, et al. (2008) Syndecan-4 regulates subcellular localization of mTOR Complex2 and Akt activation in a PKCalpha-dependent manner in endothelial cells. Mol Cell 32, 140-9
18851840   Curated Info

45

Majumder PK, et al. (2008) A prostatic intraepithelial neoplasia-dependent p27 Kip1 checkpoint induces senescence and inhibits cell proliferation and cancer progression. Cancer Cell 14, 146-55
18691549   Curated Info

46

Possemato A (2008) CST Curation Set: 4745; Year: 2008; Biosample/Treatment: cell line, 3T3-L1/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-Akt Substrate (RXRXXS/T) (110B7) Rabbit mAb Cat#: 9614, PTMScan(R) Phospho-Akt Substrate Motif (RXXS*/T*) Immunoaffinity Beads Cat#: 1978
Curated Info

47

Bayascas JR, et al. (2008) Mutation of the PDK1 PH domain inhibits protein kinase B/Akt, leading to small size and insulin resistance. Mol Cell Biol 28, 3258-72
18347057   Curated Info

48

Possemato A (2008) CST Curation Set: 3845; Year: 2008; Biosample/Treatment: tissue, liver/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-Akt Substrate (RXRXXS/T) (110B7) Rabbit mAb Cat#: 9614, PTMScan(R) Phospho-Akt Substrate Motif (RXXS*/T*) Immunoaffinity Beads Cat#: 1978
Curated Info

49

Possemato A (2008) CST Curation Set: 3824; Year: 2008; Biosample/Treatment: tissue, brain/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-Akt Substrate (RXRXXS/T) (110B7) Rabbit mAb Cat#: 9614, PTMScan(R) Phospho-Akt Substrate Motif (RXXS*/T*) Immunoaffinity Beads Cat#: 1978
Curated Info

50

Possemato A (2008) CST Curation Set: 3831; Year: 2008; Biosample/Treatment: tissue, heart/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-Akt Substrate (RXRXXS/T) (110B7) Rabbit mAb Cat#: 9614, PTMScan(R) Phospho-Akt Substrate Motif (RXXS*/T*) Immunoaffinity Beads Cat#: 1978
Curated Info

51

Kumar A, et al. (2008) Muscle-specific deletion of rictor impairs insulin-stimulated glucose transport and enhances Basal glycogen synthase activity. Mol Cell Biol 28, 61-70
17967879   Curated Info

52

Zaru R, et al. (2007) The MAPK-activated kinase Rsk controls an acute Toll-like receptor signaling response in dendritic cells and is activated through two distinct pathways. Nat Immunol 8, 1227-35
17906627   Curated Info

53

Shahid G, Hussain T (2007) GRK2 negatively regulates glycogen synthesis in mouse liver FL83B cells. J Biol Chem 282, 20612-20
17517892   Curated Info

54

Guo A (2007) CST Curation Set: 2901; Year: 2007; Biosample/Treatment: tissue, brain/-; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-(Ser/Thr) PKD Substrate Antibody Cat#: 4381, PTMScan(R) Phospho-PKD Substrate Motif (LXRXXpS/pT) Immunoaffinity Beads Cat#: 1986
Curated Info

55

Guo A (2007) CST Curation Set: 2902; Year: 2007; Biosample/Treatment: tissue, brain/-; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-(Ser/Thr) PKD Substrate Antibody Cat#: 4381, PTMScan(R) Phospho-PKD Substrate Motif (LXRXXpS/pT) Immunoaffinity Beads Cat#: 1986
Curated Info

56

Toole BJ, Cohen PT (2007) The skeletal muscle-specific glycogen-targeted protein phosphatase 1 plays a major role in the regulation of glycogen metabolism by adrenaline in vivo. Cell Signal 19, 1044-55
17257813   Curated Info

57

Cosentino C, et al. (2007) p85 regulatory subunit of PI3K mediates cAMP-PKA and estrogens biological effects on growth and survival. Oncogene 26, 2095-103
17016431   Curated Info

58

Hilfiker-Kleiner D, et al. (2007) A cathepsin D-cleaved 16 kDa form of prolactin mediates postpartum cardiomyopathy. Cell 128, 589-600
17289576   Curated Info

59

Nascimento EB, et al. (2006) Insulin-mediated phosphorylation of the proline-rich Akt substrate PRAS40 is impaired in insulin target tissues of high-fat diet-fed rats. Diabetes 55, 3221-8
17130464   Curated Info

60

Limesand KH, Schwertfeger KL, Anderson SM (2006) MDM2 is required for suppression of apoptosis by activated Akt1 in salivary acinar cells. Mol Cell Biol 26, 8840-56
16982679   Curated Info

61

Sakamoto K, et al. (2006) Role of Akt2 in contraction-stimulated cell signaling and glucose uptake in skeletal muscle. Am J Physiol Endocrinol Metab 291, E1031-7
16803855   Curated Info

62

Padmanabhan J, Levy M, Dickson DW, Potter H (2006) Alpha1-antichymotrypsin, an inflammatory protein overexpressed in Alzheimer's disease brain, induces tau phosphorylation in neurons. Brain 129, 3020-34
16987932   Curated Info

63

Zhang HH, et al. (2006) S6K1 regulates GSK3 under conditions of mTOR-dependent feedback inhibition of Akt. Mol Cell 24, 185-97
17052453   Curated Info

64

Jacinto E, et al. (2006) SIN1/MIP1 maintains rictor-mTOR complex integrity and regulates Akt phosphorylation and substrate specificity. Cell 127, 125-37
16962653   Curated Info

65

Gärtner A, Huang X, Hall A (2006) Neuronal polarity is regulated by glycogen synthase kinase-3 (GSK-3beta) independently of Akt/PKB serine phosphorylation. J Cell Sci 119, 3927-34
16954147   Curated Info

66

De Sarno P, et al. (2006) In vivo regulation of GSK3 phosphorylation by cholinergic and NMDA receptors. Neurobiol Aging 27, 413-22
16464655   Curated Info

67

Li L, et al. (2006) Protein kinase C negatively regulates Akt activity and modifies UVC-induced apoptosis in mouse keratinocytes. J Biol Chem 281, 3237-43
16338928   Curated Info

68

Kaidanovich-Beilin O, Eldar-Finkelman H (2006) Long-term treatment with novel glycogen synthase kinase-3 inhibitor improves glucose homeostasis in ob/ob mice: molecular characterization in liver and muscle. J Pharmacol Exp Ther 316, 17-24
16169938   Curated Info

69

McManus EJ, et al. (2005) Role that phosphorylation of GSK3 plays in insulin and Wnt signalling defined by knockin analysis. EMBO J 24, 1571-83
15791206   Curated Info

70

Ho L, et al. (2004) Diet-induced insulin resistance promotes amyloidosis in a transgenic mouse model of Alzheimer's disease. FASEB J 18, 902-4
15033922   Curated Info

71

Murata H, Hresko RC, Mueckler M (2003) Reconstitution of phosphoinositide 3-kinase-dependent insulin signaling in a cell-free system. J Biol Chem 278, 21607-14
12682058   Curated Info

72

Pankov R, et al. (2003) Specific beta1 integrin site selectively regulates Akt/protein kinase B signaling via local activation of protein phosphatase 2A. J Biol Chem 278, 18671-81
12637511   Curated Info

73

Fang X, et al. (2002) Convergence of multiple signaling cascades at glycogen synthase kinase 3: Edg receptor-mediated phosphorylation and inactivation by lysophosphatidic acid through a protein kinase C-dependent intracellular pathway. Mol Cell Biol 22, 2099-110
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