Ser363
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Home > Phosphorylation Site Page: > Ser363  -  p90RSK (rat)

Site Information
TSRtPRDsPGIPPsA   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 447989

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 10 ) , immunoprecipitation ( 8 ) , mass spectrometry ( 2 , 3 ) , mutation of modification site ( 7 , 8 , 10 ) , phospho-antibody ( 1 , 4 , 5 , 6 , 7 , 9 , 10 ) , western blotting ( 1 , 4 , 5 , 6 , 7 , 8 )
Relevant cell line - cell type - tissue:
'neuron, hippocampal'-brain ( 9 ) , 293 (epithelial) ( 8 ) , BLMVEC (endothelial) ( 7 ) , COS (fibroblast) ( 10 ) , HeLa (cervical) ( 1 ) , intestine ( 2 ) , liver ( 2 , 3 , 6 ) , RIE (epithelial) ( 5 ) , RIE (epithelial) [PPAR-gamma (mouse)] ( 5 ) , spleen ( 2 ) , stomach ( 2 ) , vascular smooth muscle cell ('muscle, smooth') ( 4 )

Upstream Regulation
Regulatory protein:
ERK2 (rat) ( 5 ) , ERK5 (human) ( 7 ) , MEK5 (human) ( 7 )
Kinases, in vitro:
ERK1 (human) ( 10 ) , ERK2 (human) ( 10 )
Treatments:
culturing_of_cells ( 6 ) , EGF ( 1 ) , IL-1b ( 4 ) , ischemia/reperfusion ( 6 ) , laminar flow ( 7 ) , LY294002 ( 6 ) , mutation ( 1 ) , PD98059 ( 4 , 6 , 10 ) , phorbol_ester ( 10 ) , rapamycin ( 3 ) , RS5444 ( 5 ) , SB202190 ( 6 ) , SB203580 ( 6 ) , serum ( 8 ) , TGF-beta ( 9 ) , U0126 ( 4 , 9 ) , vanadate ( 6 ) , VEGF ( 6 )

Downstream Regulation
Effects of modification on p90RSK:
activity, induced ( 10 )

References 

1

Gao X, Chaturvedi D, Patel TB (2012) Localization and retention of p90 ribosomal S6 kinase 1 in the nucleus: implications for its function. Mol Biol Cell 23, 503-15
22130794   Curated Info

2

Lundby A, et al. (2012) Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues. Nat Commun 3, 876
22673903   Curated Info

3

Demirkan G, et al. (2011) Phosphoproteomic Profiling of In Vivo Signaling in Liver by the Mammalian Target of Rapamycin Complex 1 (mTORC1). PLoS One 6, e21729
21738781   Curated Info

4

Xu S, Bayat H, Hou X, Jiang B (2006) Ribosomal S6 kinase-1 modulates interleukin-1beta-induced persistent activation of NF-kappaB through phosphorylation of IkappaBbeta. Am J Physiol Cell Physiol 291, C1336-45
16822942   Curated Info

5

Chen L, et al. (2006) Peroxisome proliferator-activated receptor gamma promotes epithelial to mesenchymal transformation by Rho GTPase-dependent activation of ERK1/2. J Biol Chem 281, 24575-87
16815847   Curated Info

6

Ohi N, et al. (2006) Maintenance of Bad phosphorylation prevents apoptosis of rat hepatic sinusoidal endothelial cells in vitro and in vivo. Am J Pathol 168, 1097-106
16565486   Curated Info

7

Pi X, Yan C, Berk BC (2004) Big mitogen-activated protein kinase (BMK1)/ERK5 protects endothelial cells from apoptosis. Circ Res 94, 362-9
14670836   Curated Info

8

Cavet ME, Lehoux S, Berk BC (2003) 14-3-3beta is a p90 ribosomal S6 kinase (RSK) isoform 1-binding protein that negatively regulates RSK kinase activity. J Biol Chem 278, 18376-83
12618428   Curated Info

9

Zhu Y, et al. (2002) Transforming growth factor-beta 1 increases bad phosphorylation and protects neurons against damage. J Neurosci 22, 3898-909
12019309   Curated Info

10

Dalby KN, et al. (1998) Identification of regulatory phosphorylation sites in mitogen-activated protein kinase (MAPK)-activated protein kinase-1a/p90rsk that are inducible by MAPK. J Biol Chem 273, 1496-505
9430688   Curated Info